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Home / Equine Research Bank / Am J Vet Res / Concentrations of serum amyloid A in serum and synovial flui...
American journal of veterinary research2006; 67(10); 1738-1742; doi: 10.2460/ajvr.67.10.1738

Concentrations of serum amyloid A in serum and synovial fluid from healthy horses and horses with joint disease.

Abstract: To determine serum amyloid A (SAA) concentrations in serum and synovial fluid from healthy horses and horses with joint disease and assess the effect of repeated arthrocentesis on SAA concentrations in synovial fluid. Animals-10 healthy horses and 21 horses with various types of joint disease. Methods: Serum and synovial fluid samples were obtained from each horse. In 5 of the 10 healthy horses, arthrocentesis was repeated 9 times. Concentrations of SAA were determined via immunoturbidometry. Results: Serum and synovial fluid SAA concentrations were less than the assay detection limit in healthy horses and did not change in response to repeated arthrocentesis. Synovial fluid SAA concentrations were significantly higher in horses with suspected bacterial joint contamination or infectious arthritis, or tenovaginitis than in healthy controls, and serum concentrations were significantly higher in horses with infectious conditions than in the other groups. Neither serum nor synovial fluid SAA concentrations in horses with low-inflammation joint conditions differed significantly from those in healthy controls. Concentrations of SAA and total protein in synovial fluid were significantly correlated. Conclusions: Synovial fluid SAA concentration was a good marker of infectious arthritis and tenovaginitis and appeared to reflect changes in inflammatory activity. The advantages of use of SAA as a marker include the ease and speed of measurement and the fact that concentrations in synovial fluid were not influenced by repeated arthrocentesis in healthy horses. Further study of the SAA response in osteoarthritic joints to assess its usefulness in diagnosis and monitoring of osteoarthritis is warranted.
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Publication Date: 2006-10-04 PubMed ID: 17014325DOI: 10.2460/ajvr.67.10.1738Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research was conducted to analyze the levels of serum amyloid A (SAA) in the blood and joint fluids of healthy horses as well as those suffering from various joint diseases. It examined the impact of repeated arthrocentesis and found SAA to be an effective marker of infectious arthritis and tenovaginitis.

Methodology of the Research

  • The study was conducted on 10 healthy horses and 21 horses with different kinds of joint diseases.
  • Both blood and synovial fluid samples were collected from each horse. Half of the healthy horses underwent repeated arthrocentesis, a procedure to remove the joint fluid, nine times in total.
  • The levels of SAA in these samples were ascertained via a process known as immunoturbidometry.

Research Findings

  • The SAA levels in both serum and synovial fluid were found to be lower than the assay detection limit in healthy horses. These levels did not vary in response to regular arthrocentesis.
  • Increased SAA levels in the synovial fluid were observed in horses with suspected bacterial joint contamination, infectious arthritis, or tenovaginitis as compared to the healthy horses.
  • Those horses suffering from infectious conditions showed significantly higher levels of SAA in their serum in comparison to other groups.
  • Horses with low-inflammation joint conditions didn’t show any significant difference in serum or synovial fluid SAA levels, when compared to healthy horses.
  • The study discovered a significant correlation between SAA and total protein concentrations in the synovial fluid.

Conclusion of the Research

  • The concentration of SAA in the synovial fluid proved to be an effective marker for infectious arthritis and tenovaginitis, indicating changes in inflammatory activity.
  • The benefits of using SAA as an indicator include ease and speed of measurement, along with the characteristic that its concentration in joint fluid doesn’t alter with repeated arthrocentesis in healthy horses.
  • The study suggested the necessity of further research into the SAA response in joints affected by osteoarthritis, to explore its potential use in diagnosing and monitoring the progression of osteoarthritis.

Cite This Article

APA
Jacobsen S, Thomsen MH, Nanni S. (2006). Concentrations of serum amyloid A in serum and synovial fluid from healthy horses and horses with joint disease. Am J Vet Res, 67(10), 1738-1742. https://doi.org/10.2460/ajvr.67.10.1738

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 67
Issue: 10
Pages: 1738-1742

Researcher Affiliations

Jacobsen, Stine
  • Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlaegevej 48, DK-1870 Frederiksberg C, Copenhagen, Denmark.
Thomsen, Maj Halling
    Nanni, Simone

      MeSH Terms

      • Animals
      • Arthritis, Infectious / blood
      • Arthritis, Infectious / metabolism
      • Arthritis, Infectious / veterinary
      • Health
      • Horse Diseases / blood
      • Horse Diseases / metabolism
      • Horses
      • Serum Amyloid A Protein / analysis
      • Synovial Fluid / metabolism
      • Tenosynovitis / blood
      • Tenosynovitis / metabolism
      • Tenosynovitis / veterinary

      Citations

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