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Home / Equine Research Bank / BMC Vet Res / Conjunctival aerobic bacterial flora in healthy Silesian foa...
BMC veterinary research2018; 14(1); 261; doi: 10.1186/s12917-018-1598-6

Conjunctival aerobic bacterial flora in healthy Silesian foals and adult horses in Poland.

Abstract: Commensal bacterial and fungal flora of the conjunctival sac has been described in horses and other animals. The identification of commensal flora of the conjunctival sac may aid in the diagnosis of ocular inflammatory diseases, such as conjunctivitis or more severe ulcerative keratitis, common in horses. Moreover, damage of ocular protective barriers may lead to an opportunistic infection. The study was carried out in Silesian horses kept at a single breeding center in South-western Poland, in order to limit any breed-dependant and climate-dependant variables affecting the results. Following an ophthalmic examination that revealed no abnormalities, sterile swabs were collected from conjunctival sac in 26 adult horses and 11 foals. The obtained swabs were subjected to bacterial culture testing. In case of Staphylococcus spp. isolation, susceptibility to methicillin was evaluated. Results: Forty- three bacterial isolates, representing eleven genera of bacteria were cultured from 30 (81%) horses. Gram-positive bacteria were the dominant isolates (72%) (p < 0.001). The most commonly isolated Gram-positive bacteria were Staphylococcus spp., while Moraxella spp. were the most frequently isolated Gram-negative bacteria. There was no significant influence of sex and age on the frequency and type of microbial isolates. Conclusions: Commensal flora is present in the conjunctival sac of healthy horses in Poland. Age does not affect the abundance and type of microbial isolates.
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Publication Date: 2018-08-31 PubMed ID: 30170594PubMed Central: PMC6119321DOI: 10.1186/s12917-018-1598-6Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study conducted on the bacterial and fungal flora present in the conjunctival sac of healthy Silesian horses in Poland, and how this information can assist in diagnosing ocular inflammatory diseases.

Introduction and Purpose of Research

  • The authors of this study shed light on the bacterial and fungal flora present in the conjunctival sac of horses. They noted that understanding these microorganisms can aid in diagnosing ocular (eye-related) inflammatory diseases such as conjunctivitis or ulcerative keratitis, which are common in horses. The study also examined the possibility of opportunistic infections occurring due to damage of ocular protective barriers.
  • The researchers chose to carry out their study on Silesian horses in a single breeding center in South-western Poland in order to limit variables that would be dependent on breed and climate.

Research Methodology

  • An ophthalmic examination was initially carried out on the horses to ensure no abnormalities were present. Once this was established, sterile swabs were collected from the conjunctival sac of 26 adult horses and 11 foals.
  • The swabs obtained were subjected to bacterial culture testing to identify the bacteria present.
  • For Staphylococcus spp., the researchers also assessed the bacteria’s susceptibility to methicillin – an antibiotic commonly used to treat bacterial infections.

Results of the Study

  • The cultivation of the swabs resulted in forty-three bacterial isolates, representing eleven genera (types) of bacteria in 30 (81%) of the horses.
  • In terms of types of bacteria, Gram-positive bacteria were the dominant isolates found (72%), with the most commonly isolated Gram-positive bacteria being Staphylococcus spp. Among the Gram-negative bacteria, Moraxella spp were most frequently isolated.
  • The researchers also checked whether the horses’ sex and age had any significant influence on the frequency and type of microbial isolates, but found it did not.

Conclusion

  • The research concluded that commensal flora (the natural, harmless microbial population) is present in the conjunctival sac of healthy horses in Poland, irrespective the horse’s age, implying that age does not impact the abundance and type of microbial isolates.

Cite This Article

APA
Zak A, Siwinska N, Slowikowska M, Borowicz H, Ploneczka-Janeczko K, Chorbinski P, Niedzwiedz A. (2018). Conjunctival aerobic bacterial flora in healthy Silesian foals and adult horses in Poland. BMC Vet Res, 14(1), 261. https://doi.org/10.1186/s12917-018-1598-6

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 261

Researcher Affiliations

Zak, A
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wroclaw, Poland. agnieszka.zak@upwr.edu.pl.
Siwinska, N
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wroclaw, Poland.
Slowikowska, M
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wroclaw, Poland.
Borowicz, H
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wroclaw, Poland.
Ploneczka-Janeczko, K
  • Department of Epizootiology with Clinic for Birds and Exotic Animals, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366, Wroclaw, Poland.
Chorbinski, P
  • Department of Epizootiology with Clinic for Birds and Exotic Animals, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366, Wroclaw, Poland.
Niedzwiedz, A
  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366, Wroclaw, Poland.

MeSH Terms

  • Age Factors
  • Animals
  • Bacteria / classification
  • Bacteria / isolation & purification
  • Female
  • Horses / microbiology
  • Lacrimal Apparatus / microbiology
  • Male
  • Methicillin Resistance / genetics
  • Poland
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: In accordance with the Experiments on Animals Act from January 15th 2015 (Journal of Laws of the Republic of Poland, 2015, item. 266), concerning the welfare of the animals used for research or teaching purposes, the provisions shall not apply to: 1. veterinary services as defined by the Act from December 18th 2003 concerning veterinary practices (Journal of Laws from 2004, No. 11, item 95 as amended in item 3), as well as agricultural activity, raising and breeding livestock according to the Animal Welfare Act, not designed to carry out medical procedures; 2. clinical veterinary studies carried out according to Article 37ah-37ak of the Act from September 6th 2001 – Pharmaceutical Law (Journal of Laws from 2008, No. 45, item 271 as amended in item 4); 3. activity aimed at identifying animals; 4. capturing wild animals for biometric and systematic assessment; 5. veterinary procedures which to not cause pain, suffering, distress or permanent health impairment equal to or more invasive than the insertion of a needle. Hence, the study entitled “The bacteriological examination of conjunctival swabs in healthy Silesian adult horses and foals.” does not require the approval of the Ethics Committee. All procedures were performed during the study with owner consent. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. None of the aforementioned companies had any direct or indirect involvement in the development of the manuscript and the works reflects the opinions of the authors. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 32 references
  1. Keller RL, Hendrix DVH. Bacterial isolates and antimicrobial susceptibilities in equine bacterial ulcerative keratitis (1993-2004). Equine Vet J 2005;37(3):207–211.
    doi: 10.2746/0425164054530731pubmed: 15892227google scholar: lookup
  2. Gilger BC. Immunology of the ocular surface. Vet Clin Small Anim 2008;38:223–231.
    doi: 10.1016/j.cvsm.2007.11.004pubmed: 18299004google scholar: lookup
  3. Gemensky-Metzler AJ, Wilkie DA, Kowalski JJ, Schmall LM, Willis A, Yamagata M. Changes in bacterial and fungal ocular flora of clinically normal horses following experimental application of topical antimicrobial or antimicrobial-corticosteroid ophthalmic preparations. Am J Vet Res 2005;66:800–811.
    doi: 10.2460/ajvr.2005.66.800pubmed: 15934607google scholar: lookup
  4. Halbert SP. Inhibitory properties of the ocular flora. In: Lacatcher-Khorazo D, Seegal BC, editors. Microbiology of the eye. St Louis: Mosby; 1972. pp. 24–40.
  5. Moore CP, Heller N, Majors LJ, Whitley RD, Burgees EC, Weber J. Prevalence of ocular microorganisms in hospitalized and stabled horses. Am J Vet Res 1988;49:773–779.
    pubmed: 3400913
  6. Whitley RD, Burgess EC, Moore CP. Microbial isolates of the normal equine eye. Equine vet J Suppl 1983;2:138–140.
  7. Andrew SE, Nguyen A, Jones GL, Brooks DE. Seasonal effects on the aerobic bacterial and fungal conjunctival flora of normal thoroughbred brood mares in Florida. Vet Ophthalmol 2003;6:45–50.
    doi: 10.1046/j.1463-5224.2003.00265.xpubmed: 12641842google scholar: lookup
  8. Johns IC, Baxter K, Booler H, Hicks C, Menzies-Gow N. Conjunctival bacterial and fungal flora in healthy horses in the UK. Vet Ophthalmol 2011;14(3):195–199.
    doi: 10.1111/j.1463-5224.2010.00867.xpubmed: 21521444google scholar: lookup
  9. Whitley RD, Moore CP. Microbiology of the equine eye in health and disease. Vet Clin N Am-Large 1984;6(3):451–466.
    pubmed: 6393541
  10. Sgorbini M, Barsotti G, Nardoni S, Mancianti F, Rossi S, Corazza M. Fungal Flora of normal eyes in healthy newborn foals living in the same stud farm in Italy. J Equine Vet Sci 2008;28(9):540–543.
    doi: 10.1016/j.jevs.2008.07.018google scholar: lookup
  11. Rosa M, Cardozo LM, Pereira J, Brooks DE, Martins ALB, Florido PSS, Stussi JSP. Fungal flora of normal eyes of healthy horses from the state of Rio de Janeiro, Brazil. Vet Ophthalmol 2003;6(1):51–55.
    doi: 10.1046/j.1463-5224.2003.00267.xpubmed: 12641843google scholar: lookup
  12. Kiełbowicz Z, Płoneczka-Janeczko K, Bania J, Bierowiec K, Kiełbowicz M. Characteristics of the bacterial flora in the conjunctival sac of cats from Poland. J Small Anim Pract 2015;56:203–206.
    doi: 10.1111/jsap.12304pubmed: 25482666google scholar: lookup
  13. Urban M, Wyman M, Rheins M, Marraro RV. Conjunctival flora of clinically normal dogs. JAVMA 1972;161:201–206.
    pubmed: 5036187
  14. Wilcox GE. Bacterial flora of the bovine eye with special reference to the Moraxella and Neisseria. Aust Vet J 1970;46:253–257.
    doi: 10.1111/j.1751-0813.1970.tb15771.xpubmed: 4987567google scholar: lookup
  15. Leigue L, Montiani-Ferreirm F, Moore BA. Antimicrobial susceptibility and minimal inhibitory concentration of Pseudomonas aeruginosa isolated from septic ocular surface disease in different animal species. Open Veterinary Journal 2016;6(3):215–222.
    doi: 10.4314/ovj.v6i3.9pmc: PMC5133397pubmed: 27928519google scholar: lookup
  16. Moore CP, Fales WH, Whittington P, Bauer L. Bacterial and fungal isolates from Equidae with ulcerative keratitis. JAVMA 1983;182(6):600–603.
    pubmed: 6833103
  17. Sauer P, Andrew SE, Lassaline M, Gelatt KN, Denis HM. Changes in antibiotic resistance in equine bacterial ulcerative keratitis (1991–2000): 65 horses. Vet Ophthalmol 2003;6(4):309–313.
    doi: 10.1111/j.1463-5224.2003.00312.xpubmed: 14641828google scholar: lookup
  18. Hidaka S, Kobayashi M, Ando K, Fujji Y. Efficacy and safety of lomefloxacin on bacterial extraocular disease in the horse. J Vet Med Sci 2015;77(7):829–835.
    doi: 10.1292/jvms.14-0507pmc: PMC4527505pubmed: 25787926google scholar: lookup
  19. Malhotra S, Kaur N, Mehta DK. Bactericidal effect of trypan blue and fluorescein sodium in ophthalmic practice. Nepal J Ophthalmol 2012;4(1):80–83.
    pubmed: 22344002
  20. Szewczyk ME. Diagnostyka bakteriologiczna. Warszawa: Wydawnictwo Naukowe PWN; 2013.
  21. Pourmand MR, Hassanzedeh S, Mashhadi R, Askari E. Comparison of four diagnostic methods for detection of methicillin resistant Staphylococcus aureus. Iranian Journal of Microbiology 2014;6(5):341–344.
    pmc: PMC4385575pubmed: 25848525
  22. Kloos WE, Bannerman TL. Update of clinical significance of coagulaso-negative Staphylococci. Clinical Microbiology Reviews 1994;7(1):177–14023.
    doi: 10.1128/CMR.7.1.117pmc: PMC358308pubmed: 8118787google scholar: lookup
  23. Kuroda T, Kinoshita Y, Niwa H, Mizobe F, Ueno T, Kuwano A, Hatazoe T, Hobo S. Methicillin-resistant Staphylococcus aureus ulcerative keratitis in a thoroughbred racehorse. J Equine Sci 2015;26(3):95–98.
    doi: 10.1294/jes.26.95pmc: PMC4591416pubmed: 26435683google scholar: lookup
  24. Karakulska J, Fijałkowski K, Nawrotek P, Pobucewicz A, Poszumski F, Identification C-FD. Methicillin resistance of coagulase-negative staphylococci isolated from nasal cavity of healthy horses. J Microbiol 2012;50(3):444–451.
    doi: 10.1007/s12275-012-1550-6pubmed: 22752908google scholar: lookup
  25. Yasuda R, Kawano J, Onda H, Takagi M, Shimizu A, Anzai T. Methicillin-resistant coagulase-negative staphylococci isolated from healthy horses in Japan. AJVR 2000;61(11):1451–1455.
    doi: 10.2460/ajvr.2000.61.1451pubmed: 11108196google scholar: lookup
  26. Hartmann FA, Troslte SS, Klohnen AA. Isolation of methicillin-resistant Staphylococcus aureus from a postoperative wound infection in a horse. J Am Med Assoc 1997;211:590–592.
    pubmed: 9290826
  27. Shanmuganathan VA, Armstrong M, Buller A, Tullo AB. External ocular infections due to methicillin-resistant Staphylococcus aureus (MRSA). Eye 2005;19:284–291.
    doi: 10.1038/sj.eye.6701465pubmed: 15375372google scholar: lookup
  28. Blomquist PH. Methicillin-resistant Staphylococcus aureus infections of the eye and orbit. Trans Am Ophthalmol Soc 2006;104:322–345.
    pmc: PMC1809917pubmed: 17471350
  29. Brooks DE, Andrew SE, Biros DJ, Denis HM, Cutler TJ, Strubbe DT, Gelatt KN. Ulcerative keratitis caused by beta-hemolytic Streptococcus equi in 11 horses. Vet Ophthalmol 2000;3:121–125.
    doi: 10.1046/j.1463-5224.2000.00120.xpubmed: 11397293google scholar: lookup
  30. McLaughlin SA, Brightman AH, Helper LC, Manning JP, Tomes JE. Pathogenic bacteria and fungi associated with extraocular disease in the horse. J Am Vet Med Assoc 1983;182(3):241–242.
    pubmed: 6826446
  31. Lee PW, Jun AK, Cho BC. A study of microbial flora of conjunctival sac in newborns. Korean J Ophthalmol 1989;3(1):33–37.
    doi: 10.3341/kjo.1989.3.1.33pubmed: 2795940google scholar: lookup
  32. Butler JF, Garcia-Maruniak A, Meek F, Maruniak JE. Wild Florida house flies (Musca Domestica) as carriers of pathogenic bacteria. Fla Entomol 2010;93(2):218–223.
    doi: 10.1653/024.093.0211google scholar: lookup

Citations

This article has been cited 4 times.
  1. Płoneczka-Janeczko K, Armstrong E, Siemieniuch-Tartanus M, Magdziarz M. Remodelling of the healthy foal's conjunctival microbiome in the first two months of life. J Vet Res 2025 Mar;69(1):131-140.
    doi: 10.2478/jvetres-2025-0001pubmed: 40144056google scholar: lookup
  2. Li Y, Wang Y, Gao X, Luo L, Zhang B, Wang X, Li J, Wu R, He L, Li W, Qiu C. Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China. Vet Sci 2025 Jan 6;12(1).
    doi: 10.3390/vetsci12010021pubmed: 39852896google scholar: lookup
  3. Santibáñez R, Lara F, Barros TM, Mardones E, Cuadra F, Thomson P. Ocular Microbiome in a Group of Clinically Healthy Horses. Animals (Basel) 2022 Apr 7;12(8).
    doi: 10.3390/ani12080943pubmed: 35454190google scholar: lookup
  4. Fraczkowska K, Zak-Bochenek A, Siwinska N, Rypula K, Ploneczka-Janeczko K. Aerobic Commensal Conjunctival Microflora in Healthy Donkeys. Animals (Basel) 2022 Mar 17;12(6).
    doi: 10.3390/ani12060756pubmed: 35327153google scholar: lookup
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