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Theriogenology2018; 113; 8-18; doi: 10.1016/j.theriogenology.2018.02.001

Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium.

Abstract: Neutrophil extracellular traps (NETs) are DNA complexes carrying nuclear and cytoplasmic proteins, such as elastase (ELA), cathepsin-G (CAT) and myeloperoxidase (MPO). Mare endometrosis is a chronic degenerative process characterized by excessive collagen in endometrium. While NETs fight bacteria that cause endometritis, they may trigger endometrial fibrogenesis. The aim was to evaluate the in vitro effect of some NETs components on mare endometrial fibrogenesis and determine its relationship with histopathology or estrous cycle. Endometrial explants were incubated with NETs components (ELA, CAT, MPO or oxytocin). Collagen type I (COL1) protein and type I and III (COL3) gene transcription were evaluated in follicular and mid-luteal phases endometria (Kenney and Doig type I/IIA and IIB/III). Increased COL1 occurred with all NETs proteins, although endometrial response to each NETs protease depended on estrous cycle and/or endometrial category. Since ELA enhanced COL1 production, NETs persistence might be linked to endometrosis. Estrous cycle influenced COL1 protein concentration and COL3 transcripts, suggesting that follicular phase may favor endometrial collagen production. However, luteal phase endometria with moderate or severe lesions may be also susceptible to fibrotic effects of NETs constituents. These data propose that NETs involvement in chronic endometritis in mares may act as putative endometrial fibrogenic mediators.
Copyright © 2018 Elsevier Inc. All rights reserved.
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Publication Date: 2018-02-07 PubMed ID: 29452855DOI: 10.1016/j.theriogenology.2018.02.001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is focused on studying the impact of certain proteins in neutrophil extracellular traps (NETs) on the fibrogenesis of mare endometrium, and establishing its correlation with the estrous cycle and histopathology.

Introduction and Objectives

  • The study makes an effort to understand the effects of several components of neutrophil extracellular traps (NETs) on fibrogenesis in mare endometrium, the formation of excessive fibrous tissue in horses’ wombs.
  • NETs are complex structures composed of DNA and proteins that include elastase (ELA), cathepsin-G (CAT), and myeloperoxidase (MPO).
  • As these NETs can potentially trigger fibrogenesis, the researchers aim to investigate this interaction and its possible correlation with the estrous cycle (a horse’s reproductive cycle) and histopathology.

Methodology

  • The researchers extracted endometrium samples from horses during different phases of their reproductive cycles: the follicular phase and the mid-luteal phase.
  • These samples were treated with components of NETs (ELA, CAT, MPO or oxytocin) in a controlled laboratory setting.
  • The team studied the production of collagen type I (COL1) protein and the transcription of type I and III (COL3) gene after the treatment.

Findings

  • All proteins of the NETs caused an increase in COL1 production; however, the endometrial response to each NETs protease was observed to depend on the phase of the estrous cycle and/or the type of endometrium.
  • Elastase (ELA) was seen to enhance COL1 production, which indicates a link between persistence of NETs and endometrosis.
  • The estrous cycle was found to influence the concentration of COL1 protein and COL3 transcripts, implying that the follicular phase may boost collagen production in the endometrium.
  • Horse wombs in the luteal phase with moderate or severe lesions may also be susceptible to the fibrotic effects of NETs components.

Conclusion

  • The research study concluded that NETs’ involvement during chronic endometritis in mares could work as probable mediators for endometrial fibrogenesis.
  • The findings can enable new pathways for understanding and managing endometritis in horses by exploring the role played by the components of NETs.

Cite This Article

APA
Rebordão MR, Amaral A, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. (2018). Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium. Theriogenology, 113, 8-18. https://doi.org/10.1016/j.theriogenology.2018.02.001

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 113
Pages: 8-18
PII: S0093-691X(18)30043-8

Researcher Affiliations

Rebordão, Maria Rosa
  • CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal; Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Coimbra, Portugal.
Amaral, Ana
  • CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Lukasik, Karolina
  • Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland.
Szóstek-Mioduchowska, Anna
  • Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland.
Pinto-Bravo, Pedro
  • Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Coimbra, Portugal.
Galvão, António
  • CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal; Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland.
Skarzynski, Dariusz J
  • Institute of Animal Reproduction and Food Research of PAS, Olsztyn, Poland.
Ferreira-Dias, Graça
  • CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal. Electronic address: gmlfdias@fmv.ulisboa.pt.

MeSH Terms

  • Animals
  • Cell Survival
  • Collagen / physiology
  • Endometrium / drug effects
  • Endometrium / physiology
  • Estrous Cycle
  • Extracellular Traps / chemistry
  • Extracellular Traps / physiology
  • Female
  • Horses / physiology
  • Neutrophils / physiology
  • Tissue Culture Techniques / veterinary

Citations

This article has been cited 22 times.
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