Construction of a medium-density horse gene map.
Abstract: A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH(5000) panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.
Publication Date: 2006-04-01 PubMed ID: 16573529DOI: 10.1111/j.1365-2052.2005.01401.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research deals with the construction of a medium-density map of the horse genome, using genes distributed over the human genome for clarity. The researchers identified, confirmed, and localized certain equine gene orthologues on horse chromosomes, leading to the identification of homologous and conserved segments between the horse and human genomes.
Construction of Medium-Density Horse Gene Map
- The main focus of the research was to create a medium-density map of the horse genome. This was achieved by using genes that are evenly spread over the human genome. A total of 323 exonic primer pairs were used in this process.
- To accomplish the gene mapping, the team screened the INRA and CHORI-241 equine BAC libraries through a polymerase chain reaction and filter hybridization.
- From this screening, 237 BACs (Bacterial Artificial Chromosomes) containing equine gene orthologues were isolated. These orthologues were confirmed through sequencing.
- Fluorescent in situ hybridization (FISH) was applied to find the precise location of these BACs on horse chromosomes.
Assignment of Genes and Comparative Analysis
- 165 genes were assigned to the equine genomic map via radiation hybrid (RH) mapping using an equine RH(5000) panel and/or FISH mapping.
- The research conducted a comparison of the localizations of 713 genes mapped on both the horse genome and the human genome. The comparison identified 59 identical segments and 131 preserved segments.
- Two of the homologies, ECA27/HSA8 and ECA12p/HSA11p, were newly identified from this research.
Insights into Chromosome Evolution History
- The key takeaway from the research is the clarification it provides on chromosome evolution history. The identification and extended resolution of preserved and rearranged chromosomal sections deliver valuable insights into the evolutionary lineage of species.
- By identifying new homologies and a variety of conserved segments, the comparative genomic analysis within this research contributes significantly to our understanding of genetic variation and evolution.
Cite This Article
APA
Perrocheau M, Boutreux V, Chadi S, Mata X, Decaunes P, Raudsepp T, Durkin K, Incarnato D, Iannuzzi L, Lear TL, Hirota K, Hasegawa T, Zhu B, de Jong P, Cribiu EP, Chowdhary BP, Guérin G.
(2006).
Construction of a medium-density horse gene map.
Anim Genet, 37(2), 145-155.
https://doi.org/10.1111/j.1365-2052.2005.01401.x Publication
Researcher Affiliations
- Département de Génétique animale, Laboratoire de Génétique biochimique et de Cytogénétique, Centre de Recherches de Jouy, INRA, 78350, Jouy-en-Josas, France.
MeSH Terms
- Animals
- Chromosome Mapping
- Chromosomes
- Chromosomes, Artificial, Bacterial
- Evolution, Molecular
- Genetic Markers
- Genome, Human
- Horses / genetics
- Humans
- In Situ Hybridization, Fluorescence
Citations
This article has been cited 4 times.- Lewis MJ, Wagner B, Irvine RM, Woof JM. IgA in the horse: cloning of equine polymeric Ig receptor and J chain and characterization of recombinant forms of equine IgA. Mucosal Immunol 2010 Nov;3(6):610-21.
- Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome. Cytogenet Genome Res 2008;122(1):28-36.
- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
- Liao W, Collins A, Hobbs M, Khatkar MS, Luo J, Nicholas FW. A comparative location database (CompLDB): map integration within and between species. Mamm Genome 2007 May;18(5):287-99.
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