FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Theriogenology / Corpus luteal function in nonpregnant mares following intrau...
Theriogenology1994; 42(7); 1069-1083; doi: 10.1016/0093-691x(94)90855-9

Corpus luteal function in nonpregnant mares following intrauterine administration of prostaglandin E(2) or estradiol-17beta.

Abstract: The objective of this study was to test the hypothesis that intrauterine administration of prostaglandin E(2) (PGE(2)) or estradiol-17beta (E-17beta) would prolong CL function in nonpregnant mares. Nonpregnant mares were continuously infused with 240 mug/d of PGE(2), 6 mug/d of E-17beta, or vehicle (sham-treated) on Days 10 to 16 post ovulation (ovulation = Day 0), using osmotic minipumps surgically placed into the uterine lumen on Day 10 (n = 11 per group). Nonpregnant and pregnant mares served as negative and positive controls, respectively (n = 11 per group). Mares were defined as having prolonged CL function if plasma progesterone remained > 2.5 ng/ml and if ovulation did not occur on Days 9 to 30. Corpus luteal function was prolonged until Day 30 in 1 11 nonpregnant mares, 4 11 sham-treated mares, 6 11 E-17beta-treated mares, 8 11 PGE(2)-treated mares, and 11 11 pregnant mares. The incidence of prolonged CL function was similar (P=0.16) in the sham-treated and nonpregnant mares. The hypothesis that PGE(2) would prolong CL function in nonpregnant mares was supported, since the incidence of prolonged CL function was higher (P=0.003) in PGE(2)-treated versus nonpregnant mares, tended to be higher (P=0.09) in PGE(2)-versus sham-treated mares, and was not lower (P=0.11) in PGE(2)-treated versus pregnant mares. The hypothesis that E-17beta would prolong CL function in nonpregnant mares was not supported, since the incidence of prolonged CL function was not higher (P=0.34) in E-17beta-versus sham-treated mares, and was lower (P=0.02) in E-17beta-treated versus pregnant mares. These results demonstrate that intrauterine administration of a pharmacologic dose of PGE(2) initiated prolonged CL function in nonpregnant mares. Further experiments are needed to confirm the role of conceptus secretion of PGE(2) in CL maintenance, and to determine the mechanism of action of PGE(2) within the equine reproductive tract.
Get Full Text
Publication Date: 1994-01-01 PubMed ID: 16727611DOI: 10.1016/0093-691x(94)90855-9Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study evaluated whether the intrauterine administration of prostaglandin E(2) or estradiol-17beta can extend the functioning of the corpus luteum in nonpregnant horses. The hormone prostaglandin E(2) was found to support this theory, unlike estradiol-17beta.

Objective

  • The principal objective of the research was to determine if the intrauterine administration of the hormones prostaglandin E(2) (PGE(2)) or estradiol-17beta (E-17beta) could extend the function of the corpus luteum (CL) in nonpregnant mares.

Procedure

  • Nonpregnant mares were continuously administered either 240 µg/d of PGE(2), 6 µg/d of E-17beta, or a vehicle (sham treatment).
  • The administration took place from Days 10 to 16 following ovulation, using osmotic minipumps which were surgically placed into the uterine lumen on Day 10.
  • Pregnant and nonpregnant mares were used as positive and negative controls, totaling to 11 per group.
  • CL function was defined as extended if plasma progesterone remained above 2.5 ng/ml and if ovulation did not occur on Days 9 to 30.

Results

  • CL function was extended until Day 30 for 1 out of 11 nonpregnant mares, 4 out of 11 sham-treated mares, 6 out of 11 E-17beta-treated mares, 8 out of 11 PGE(2)-treated mares, and all 11 pregnant mares.
  • Statistically, the incidence of extended CL function in the nonpregnant and sham-treated mares was similar.
  • The hypothesis that PGE(2) would extend CL function in nonpregnant mares was corroborated, as mares treated with PGE(2) had a higher incidence of extended CL function compared to nonpregnant mares and sham-treated mares, and a not significantly different incidence when compared with pregnant mares.
  • The hypothesis that E-17beta would extend CL function was not confirmed, i.e., there was no notable difference in the incidence of prolonged CL function between E-17beta-treated mares and sham-treated mares, and the incidence was lower in E-17beta-treated mares compared to pregnant mares.

Conclusion

  • The findings showed that intrauterine administration of a pharmacological dose of PGE(2) launched extended CL function in nonpregnant mares.
  • The researchers suggested that additional studies are necessary to validate the role of conceptus secretion of PGE(2) in CL maintenance and to unravel the mechanism of action of PGE(2) within the equine reproductive tract.

Cite This Article

APA
Vanderwall DK, Woods GL, Weber JA, Lichtenwalner AB. (1994). Corpus luteal function in nonpregnant mares following intrauterine administration of prostaglandin E(2) or estradiol-17beta. Theriogenology, 42(7), 1069-1083. https://doi.org/10.1016/0093-691x(94)90855-9

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 42
Issue: 7
Pages: 1069-1083

Researcher Affiliations

Vanderwall, D K
  • Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science University of Idaho, Moscow, ID 83844-2201 USA.
Woods, G L
    Weber, J A
      Lichtenwalner, A B

        Citations

        This article has been cited 6 times.
        1. Diel de Amorim M, Klein C, Foster R, Dong L, Lopez-Rodriguez MF, Card C. Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation.. Animals (Basel) 2022 Mar 22;12(7).
          doi: 10.3390/ani12070799pubmed: 35405789google scholar: lookup
        2. Piotrowska-Tomala KK, Jonczyk AW, Szóstek-Mioduchowska AZ, Żebrowska E, Ferreira-Dias G, Skarzynski DJ. The Effects of Prostaglandin E(2) Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study.. Front Vet Sci 2021;8:753796.
          doi: 10.3389/fvets.2021.753796pubmed: 35242830google scholar: lookup
        3. Klohonatz KM, Coleman SJ, Islas-Trejo AD, Medrano JF, Hess AM, Kalbfleisch T, Thomas MG, Bouma GJ, Bruemmer JE. Coding RNA Sequencing of Equine Endometrium during Maternal Recognition of Pregnancy.. Genes (Basel) 2019 Sep 25;10(10).
          doi: 10.3390/genes10100749pubmed: 31557877google scholar: lookup
        4. Klohonatz KM, Nulton LC, Hess AM, Bouma GJ, Bruemmer JE. The role of embryo contact and focal adhesions during maternal recognition of pregnancy.. PLoS One 2019;14(3):e0213322.
          doi: 10.1371/journal.pone.0213322pubmed: 30835748google scholar: lookup
        5. Aurich C, Budik S. Early pregnancy in the horse revisited - does exception prove the rule?. J Anim Sci Biotechnol 2015;6:50.
          doi: 10.1186/s40104-015-0048-6pubmed: 26635959google scholar: lookup
        6. Szóstek AZ, Galvão AM, Hojo T, Okuda K, Skarzynski DJ. Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids.. Mediators Inflamm 2014;2014:208103.
          doi: 10.1155/2014/208103pubmed: 24719522google scholar: lookup
        Subscribe to our newsletter
        Join 99,383 horse owners like you who receive our email updates on horse health and nutrition.