Correlation between DNA restriction fragment length polymorphisms in Leptospira interrogans serovar pomona type kennewicki and host animal source.
Abstract: Isolates (n = 147) of Leptospira interrogans serovar pomona type kennewicki from cattle, swine, horses, and wildlife were analyzed by DNA restriction endonuclease analysis. Restriction fragment length polymorphisms were identified in DNA digested with HpaII, and the restriction fragment length polymorphisms were correlated with the host animal source of the isolates. These results will be useful in understanding the epidemiology of serovar pomona infections in livestock.
Publication Date: 1996-02-01 PubMed ID: 8789028PubMed Central: PMC228810DOI: 10.1128/jcm.34.2.424-425.1996Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research investigates any correlations between specific patterns observed in the DNA structure of Leptospira interrogans serovar pomona type kennewicki, a bacterial species, and the host animals they are isolated from. The study findings could provide insights into the spread and behavior of this species in livestock.
Leptospira Interrogans Serovar Pomona Type Kennewicki
This research involves a type of bacteria known as Leptospira interrogans serovar pomona type kennewicki. This bacterium is known to cause various infections in livestock such as cattle, swine, horses, and wildlife.
- This particular study analyzed a total of 147 isolates of this serovar from the mentioned host animals.
- Understanding the genetic variations in this bacteria depending on host source can contribute to understanding its epidemiology, or how it spreads and behaves.
DNA Restriction Endonuclease Analysis
To identify any correlations between the bacteria’s DNA and its host, the researchers used a technique called DNA restriction endonuclease analysis.
- This technique involves using enzymes (restriction endonucleases) to cut DNA at specific sequences.
- In this study, the enzyme HpaII was used to cut the DNA of the bacterium, resulting in fragments of varying lengths.
- These variation in fragment lengths (restriction fragment length polymorphisms or RFLPs) can reveal genetic differences between samples.
Correlation with Host Animal Source
The next step was to see if the identified RFLPs were in any way related to the host animal source of the bacteria.
- The idea here is that different host animals might have different strains of the bacterium, potentially identifiable through their unique RFLPs.
- Understanding these correlations could be crucial in controlling and preventing outbreaks of infections caused by this bacterium in livestock.
Significance of Study
The results of this study have promising implications for understanding and managing serovar pomona infections in livestock.
- By identifying any correlations between the bacteria’s DNA structure and its host animal, the researchers can predict the potential spread and behavior of this bacterium in different types of livestock.
- This could potentially lead to more targeted and effective methods for controlling and preventing these bacterial infections in livestock in the future.
Cite This Article
APA
Bolin CA, Zuerner RL.
(1996).
Correlation between DNA restriction fragment length polymorphisms in Leptospira interrogans serovar pomona type kennewicki and host animal source.
J Clin Microbiol, 34(2), 424-425.
https://doi.org/10.1128/jcm.34.2.424-425.1996 Publication
Researcher Affiliations
- Leptospirosis and Mycobacteriosis Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA. a03rllmr@attmail.com
MeSH Terms
- Animals
- Animals, Wild / microbiology
- Cattle / microbiology
- DNA, Bacterial / genetics
- DNA, Bacterial / isolation & purification
- Disease Outbreaks / veterinary
- Horses / microbiology
- Leptospira interrogans / classification
- Leptospira interrogans / genetics
- Leptospira interrogans / isolation & purification
- Molecular Epidemiology
- Polymorphism, Restriction Fragment Length
- Serotyping
- Swine / microbiology
- Weil Disease / epidemiology
- Weil Disease / microbiology
- Weil Disease / veterinary
- Zoonoses / epidemiology
- Zoonoses / microbiology
References
This article includes 12 references
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Citations
This article has been cited 8 times.- Putz EJ, Nally JE. Investigating the Immunological and Biological Equilibrium of Reservoir Hosts and Pathogenic Leptospira: Balancing the Solution to an Acute Problem?. Front Microbiol 2020;11:2005.
- Zarantonelli L, Suanes A, Meny P, Buroni F, Nieves C, Salaberry X, Briano C, Ashfield N, Da Silva Silveira C, Dutra F, Easton C, Fraga M, Giannitti F, Hamond C, Macías-Rioseco M, Menéndez C, Mortola A, Picardeau M, Quintero J, Ríos C, Rodríguez V, Romero A, Varela G, Rivero R, Schelotto F, Riet-Correa F, Buschiazzo A. Isolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis. PLoS Negl Trop Dis 2018 Sep;12(9):e0006694.
- Arent ZJ, Gilmore C, San-Miguel Ayanz JM, Neyra LQ, García-Peña FJ. Molecular Epidemiology of Leptospira Serogroup Pomona Infections Among Wild and Domestic Animals in Spain. Ecohealth 2017 Mar;14(1):48-57.
- Zuerner RL, Alt DP. Variable nucleotide tandem-repeat analysis revealing a unique group of Leptospira interrogans serovar Pomona isolates associated with California sea lions. J Clin Microbiol 2009 Apr;47(4):1202-5.
- Michel V, Ruvoen-Clouet N, Menard A, Sonrier C, Fillonneau C, Rakotovao F, Ganière JP, André-Fontaine G. Role of the coypu (Myocastor coypus) in the epidemiology of leptospirosis in domestic animals and humans in France. Eur J Epidemiol 2001;17(2):111-21.
- Levett PN. Leptospirosis. Clin Microbiol Rev 2001 Apr;14(2):296-326.
- Zuerner RL, Bolin CA. Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays. J Clin Microbiol 1997 Oct;35(10):2612-7.
- Wang W, Gao Y, Ji J, Huang Z, Xiong B, Xiang S. Trends and advances in Leptospira, a bibliometric analysis. Front Microbiol 2024;15:1514738.
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