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Proceedings of the National Academy of Sciences of the United States of America1982; 79(23); 7243-7247; doi: 10.1073/pnas.79.23.7243

Correlation of parvalbumin concentration with relaxation speed in mammalian muscles.

Abstract: The physiological role of the Ca2+-binding protein parvalbumin in skeletal muscle has been investigated by measuring the parvalbumin content by HPLC in a variety of mammalian muscles, including man, and comparing the results with the respective muscle relaxation properties and fiber type compositions. The parvalbumin concentrations were highest in the skeletal muscles of the smallest animal investigated (mouse, gastrocnemius: 4.9 g/kg), which has the highest relaxation speed, and lowest in the larger animals (horse, deep gluteal muscle: less than or equal to 0.001 g/kg) and man (vastus, triceps: less than or equal to 0.001 g/kg), which have much lower relaxation speeds. Analysis of three type-homogeneous muscles of the guinea pig revealed highest parvalbumin concentrations (0.25 g/kg) in sartorius (type IIB) and lowest concentrations (less than or equal to 0.007 g/kg) in soleus (type I), consistent with the different half-relaxation times of fast and slow muscles. Denervation of the rat extensor digitorum longus, which increases the half-relaxation time from 9.4 to 19 msec, resulted in a 20% decrease of the parvalbumin content. Given this close correlation between parvalbumin content and relaxation speed in a variety of muscles and species, we suggest that parvalbumin is involved directly in the relaxation process in fast muscles.
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Publication Date: 1982-12-01 PubMed ID: 6961404PubMed Central: PMC347315DOI: 10.1073/pnas.79.23.7243Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reveals a relationship between the concentration of parvalbumin, a calcium-binding protein, in muscles and the speed at which these muscles relax, where higher parvalbumin content associates with faster muscle relaxation.

Introduction

  • The study explores the biological role of parvalbumin, a protein that binds with calcium ions (Ca2+), in skeletal muscle functioning.
  • The research hypothesizes a correlation between parvalbumin content in muscles and their relaxation speed, observed across various mammals including humans.

Methodology

  • High Performance Liquid Chromatography (HPLC) was used to quantify the parvalbumin content in different types of muscles across mammalian species, including humans.
  • These observations were then compared with the respective muscle relaxation properties and fiber type compositions.

Results

  • Findings showed that parvalbumin concentrations were highest in the skeletal muscles of smaller animals (mouse), which demonstrated the highest relaxation speed.
  • In contrast, larger animals (horse) and man, which have lower muscle relaxation speed, showed the lowest concentrations of parvalbumin.
  • Analysis of parvalbumin concentrations in various muscles in a guinea pig revealed the highest level in the sartorius muscle (type IIB) and the lowest in the soleus muscle (type I), consistent with the different half-relaxation times of fast and slow muscles.

Additional Experiments

  • When the rat extensor digitorum longus was denervated, thereby increasing its half-relaxation time, there was a 20% decrease in the parvalbumin content.

Conclusion

  • Taken together, the results suggest that parvalbumin content may directly impact the relaxation speed in fast muscles across various species.

Cite This Article

APA
Heizmann CW, Berchtold MW, Rowlerson AM. (1982). Correlation of parvalbumin concentration with relaxation speed in mammalian muscles. Proc Natl Acad Sci U S A, 79(23), 7243-7247. https://doi.org/10.1073/pnas.79.23.7243

Publication

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
NlmUniqueID: 7505876
Country: United States
Language: English
Volume: 79
Issue: 23
Pages: 7243-7247

Researcher Affiliations

Heizmann, C W
    Berchtold, M W
      Rowlerson, A M

        MeSH Terms

        • Animals
        • Calcium / physiology
        • Guinea Pigs
        • Horses
        • Humans
        • In Vitro Techniques
        • Mice
        • Muscle Contraction
        • Muscle Denervation
        • Muscle Proteins / physiology
        • Muscle Relaxation
        • Parvalbumins / physiology
        • Rats
        • Time Factors

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