Cross reaction of recombinant equine infectious anemia virus antigen to heterologous strains and application for serological survey among horses in the field.
Abstract: Cross reactivity of equine infectious anemia virus (EIAV) antigen prepared using a recombinant baculovirus containing the p26 gene of strain P337-V70 was examined by the agar gel immunodiffusion (AGID) test and enzyme-linked immunosorbent assay (ELISA). Serum samples serially collected from 13 horses experimentally infected with six different EIAV strains (two or three horses per strain) were subjected to the test. Positive reactions were observed in the AGID test and ELISA before or soon after the first feverish period and continued persistently in most of the horses. The results with recombinant antigens were essentially the same as those with the virion antigen prepared from horse cell cultures both in the AGID test and ELISA. The reactivities of the antigens were further compared using serum samples collected from horses in 1999 in certain districts of Mongolia where equine infectious anemia has been prevalent, and from horses in Japan in 1973 when EIA had not been eliminated completely from Japanese horses. These results were completely concurrent. Generally, recombinant antigens have high specificity but low cross reactivity to heterologous strains. However, the present study showed that the recombinant EIAV p26 antigen has cross reactivity to the heterologous strain and is useful for diagnosis of EIA in the field.
Publication Date: 2001-03-29 PubMed ID: 11270606DOI: 10.1111/j.1348-0421.2001.tb01273.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Antigen
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Disease Surveillance
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Infectious Anemia
- Field Study
- Genetics
- Horses
- Immunology
- Infection
- Recombinant Proteins
- Serological Surveys
- Veterinary Medicine
- Veterinary Research
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research evaluates the effectiveness of an antigen, engineered through recombinant baculovirus, in identifying different strains of Equine Infectious Anemia Virus (EIAV) across different horses.
Research Methodology
- The study examined the cross-reactivity of an antigen specifically developed for the P337-V70 strain of EIAV. The antigen was created through a recombinant baculovirus, a type of biological carrier used for genetic material.
- This antigen was then tested against serum samples from 13 horses that were experimentally infected with six different strains of EIAV. Two to three horses were allocated per strain.
- The researchers used both the Agar Gel Immunodiffusion (AGID) test and the enzyme-linked immunosorbent assay (ELISA) to assess the effectiveness of this recombinant antigen in diagnosing different strains of the virus.
Findings and Results
- Positive reactions were observed in both the AGID test and ELISA either immediately before or soon after the onset of fever in the majority of horses.
- Moreover, the outcomes with recombinant antigens were consistently aligned with those generated from the virion antigen, an antigen derived directly from the horse cell cultures. This similarity extended to both the AGID test and ELISA.
- Reactivity of the antigens was further analyzed using historical serum samples. These samples originated from horses in regions of Mongolia affected by an EIA outbreak in 1999, as well as samples from Japan in 1973, during a period where EIA was not completely eradicated. The findings were completely concurrent with the prior results.
Conclusion and Significance
- Contrary to the typical nature of recombinant antigens which generally have low cross-reactivity to diverse strains but high specificity, the researchers found that the developed recombinant EIAV p26 antigen exhibited cross-reactivity to different strains of the virus.
- Thus, the study demonstrated the potential of the engineered EIAV p26 antigen as a reliable diagnostic tool in detecting EIA in the field. This adaptation overcomes the traditional limitations of recombinant antigens and is of significant value in managing EIA outbreaks.
Cite This Article
APA
Sentsui H, Inoshima Y, Murakami K, Akashi H, Purevtseren B, Pagmajav O, Sugiura T.
(2001).
Cross reaction of recombinant equine infectious anemia virus antigen to heterologous strains and application for serological survey among horses in the field.
Microbiol Immunol, 45(1), 45-50.
https://doi.org/10.1111/j.1348-0421.2001.tb01273.x Publication
Researcher Affiliations
- National Institute of Animal Health, Tsukuba, Ibaraki, Japan. sentsui@niah.affrc.go.jp
MeSH Terms
- Animals
- Antibodies, Viral / immunology
- Antigens, Viral / genetics
- Antigens, Viral / immunology
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay / methods
- Enzyme-Linked Immunosorbent Assay / veterinary
- Equine Infectious Anemia / diagnosis
- Equine Infectious Anemia / immunology
- Horses
- Immunodiffusion / methods
- Recombinant Proteins / immunology
- Sensitivity and Specificity
- Viral Core Proteins / genetics
- Viral Core Proteins / immunology
Citations
This article has been cited 2 times.- Pagamjav O, Kobayashi K, Murakami H, Tabata Y, Miura Y, Boldbaatar B, Sentsui H. Serological survey of equine viral diseases in Mongolia.. Microbiol Immunol 2011 Apr;55(4):289-92.
- Alvarez I, Gutierrez G, Ostlund E, Barrandeguy M, Trono K. Western blot assay using recombinant p26 antigen for detection of equine infectious anemia virus-specific antibodies.. Clin Vaccine Immunol 2007 Dec;14(12):1646-8.
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