Cross-reactivity between a monoclonal antibody that recognizes a tumor-associated antigen on bovine lymphosarcoma cells and blood lymphocytes from various mammalian species.
Abstract: Tumor-associated antigens that are expressed in lymphosarcoma B cells of cattle with enzootic bovine leukosis had been analyzed in terms of their reactivity with 13 monoclonal antibodies (MAB). By use of flow cytometry and radioimmunoprecipitation, 1 of the MAB (c143) that recognized a tumor-associated antigen cross-reacted with blood lymphocytes (BL) from various mammalian species. By use of flow cytometry, the c143 MAB reacted with 10 to 49% of BL derived from human beings, mice, dogs, horses, pigs, llamas, sheep, goats, and cattle. Titer of the c143 MAB with BL from horses, pigs, human beings, and llamas ranged between 1:6.0 x 10(4) and 1:5.3 x 10(5); titer associated with BL of goats and sheep was 1:1.6 x 10(6); and that associated with BL of cattle was 1:4.3 x 10(7). The c143 MAB specifically immunoprecipitated 3 homologous proteins from cell extracts of caprine, ovine, and bovine BL (32-, 34-, and 36- to 37-kDa bovine proteins; 31-, 32-, and 36- to 37-kDa caprine proteins; and 31.5-, 33-, and 36- to 37-kDa ovine proteins), but none was immunoprecipitated from human, murine, canine, porcine, and llama BL. These results indicate that the avidity of the c143 MAB in binding to BL from ruminants (eg, goats, sheep, and cattle) is higher than that to BL from human beings, mice, dogs, horses, pigs, and llamas. In sheep, the c143 MAB could immunoprecipitate the aforementioned proteins from BL of the Suffolk breed, but not BL from the Corriedale breed, whereas the c143 MAB immunoprecipitated apparently identical proteins from BL of 4 breeds of cattle.
Publication Date: 1992-11-01 PubMed ID: 1466490
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research involves the study of cross-reactivity between a specific antibody (c143 MAB) that identifies a particular antigen associated with lymphosarcoma (a type of cancer) occurrence in cows and lymphocytes (a type of white blood cell) in different mammalian species.
Monoclonal Antibodies and Tumor-Associated Antigens
- The study focuses on tumor-associated antigens. These are substances produced by cancer cells that can activate an immune response. In this particular case, the antigens are expressed in lymphosarcoma B cells of cattle suffering from enzootic bovine leukosis, a type of leukemia.
- To study these antigens, the researchers used 13 different types of monoclonal antibodies(MAB). Monoclonal antibodies are lab-made molecules engineered to serve as substitute antibodies. In this study, one of them (c143) recognised a specific tumor-associated antigen and also reacted with blood lymphocytes from different mammalian species.
Monoclonal Antibody c143 Reactivity
- Through flow cytometry (a technique used to measure physical and chemical characteristics of cells) and radioimmunoprecipitation (a method used to detect specific proteins), the researchers found that the c143 MAB cross-reacted with blood lymphocytes (BL) of humans, mice, dogs, horses, pigs, llamas, sheep, goats, and cattle.
- The c143 MAB bound to 10 to 49% of the blood lymphocytes from these species. The binding strength, referred to as titer, varied between these species, with the strongest binding occurring with cattle, goats, and sheep blood lymphocytes.
Immunoprecipitation and Binding Avidity
- The c143 MAB was able to precipitate certain proteins specifically from blood lymphocytes of goats, sheep, and cattle but could not do the same with human, mice, dogs, pigs, and llamas blood lymphocytes. Immunoprecipitation is a procedure by which proteins and peptides are isolated for analysis.
- The findings suggest that the c143 MAB binds more strongly (higher avidity) to blood lymphocytes from ruminants (goats, sheep, and cattle) than to those from humans, mice, dogs, horses, pigs, and llamas.
- Interestingly, the c143 MAB could immunoprecipitate proteins from the blood lymphocytes of the Suffolk breed of sheep but not from the Corriedale breed, indicating a breed-specific difference.
Cite This Article
APA
Aida Y, Okada K, Kageyama R, Amanuma H.
(1992).
Cross-reactivity between a monoclonal antibody that recognizes a tumor-associated antigen on bovine lymphosarcoma cells and blood lymphocytes from various mammalian species.
Am J Vet Res, 53(11), 1988-1991.
Publication
Researcher Affiliations
- Laboratory of Gene Technology and Safety, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
MeSH Terms
- Animals
- Antibodies, Monoclonal / immunology
- Antigen-Antibody Reactions
- Antigens, Neoplasm / immunology
- Camelids, New World
- Cattle
- Cross Reactions
- Dogs
- Enzootic Bovine Leukosis / immunology
- Flow Cytometry
- Goats
- Horses
- Humans
- Lymphocytes / immunology
- Mice
- Radioimmunoprecipitation Assay
- Sheep
- Swine
- Tumor Cells, Cultured
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
