Crossiella equi sp. nov., isolated from equine placentas.
Abstract: Over the course of the past decade, actinomycetes have been isolated from the placentas of horses diagnosed with nocardioform placentitis. The incidence of this infection has generally been low, with typically no more than 30 animals affected in most years, but the incidence increased through 1999, with placentas from 144 mares found to be infected. Approximately half of the cases result in loss of the foal. A typical actinomycete with branching mycelium was isolated from placental lesions, and a comparison of the sequence of the 16S rDNA gene against the public databases indicated a relationship to members of the suborder Pseudonocardineae. Phylogenetic analysis of representative isolates revealed a close relationship to Crossiella cryophila, and subsequent polyphasic comparisons determined that these isolates represent a novel species of Crossiella, for which the name Crossiella equi sp. nov. is proposed, with strain LDDC 22291-98(T) (= NRRL B-24104(T) = DSM 44580(T)) as the type strain.
Publication Date: 2003-01-02 PubMed ID: 12508885DOI: 10.1099/ijs.0.02257-0Google Scholar: Lookup
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Summary
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This research article discusses the discovery of a new bacterial species, named Crossiella equi, that was isolated from horse placentas infected with nocardioform placentitis.
Isolation of Actinomycetes
- The research carried out over the past ten years has identified a type of bacteria, known as actinomycetes, in the placentas of horses diagnosed with nocardioform placentitis.
- Nocardioform placentitis is an infection that, even though known to be relatively rare, has seen an increasing number of cases, particularly in 1999 where 144 mares’ placentas were infected.
- This infection can have serious consequences as about half of the cases resulted in the loss of the foal.
Discovery of Crossiella equi
- The researchers were able to isolate a typical actinomycete referred to as a branching mycelium from the placental lesions.
- Further analysis, including a comparison of a sequence of the 16S rDNA gene against public databases, revealed a relationship to members part of the suborder Pseudonocardineae.
- Further investigation showed a close phylogenetic relationship to an existing bacterial species called Crossiella cryophila which related to them both being of the same genus, Crossiella.
Identification of a new species
- Final results from the research led to the proposal of a new bacterial species named Crossiella equi, due to its identification in equine placentas.
- The specific strain of this species identified as LDDC 22291-98(T) (= NRRL B-24104(T) = DSM 44580(T)), has been labelled as the type strain.
Conclusion
- This research is groundbreaking in the identification of a new bacterial species that could broaden our understanding of nocardioform placentitis in horses, and can also focus on determining specific treatments or preventive measures tailored to combat Crossiella equi.
Cite This Article
APA
Donahue JM, Williams NM, Sells SF, Labeda DP.
(2003).
Crossiella equi sp. nov., isolated from equine placentas.
Int J Syst Evol Microbiol, 52(Pt 6), 2169-2173.
https://doi.org/10.1099/ijs.0.02257-0 Publication
Researcher Affiliations
- Livestock Disease Diagnostic Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40511, USA.
MeSH Terms
- Actinomycetales / classification
- Actinomycetales / genetics
- Actinomycetales / isolation & purification
- Actinomycetales / pathogenicity
- Actinomycetales Infections / microbiology
- Actinomycetales Infections / veterinary
- Animals
- DNA, Bacterial / genetics
- DNA, Ribosomal / genetics
- Fatty Acids / analysis
- Female
- Horse Diseases / microbiology
- Horses
- Microscopy, Electron, Scanning
- Molecular Sequence Data
- Phenotype
- Phylogeny
- Placenta / microbiology
- Placenta Diseases / microbiology
- Placenta Diseases / veterinary
- Pregnancy
- Pregnancy Complications, Infectious / microbiology
- Pregnancy Complications, Infectious / veterinary
- RNA, Bacterial / genetics
- RNA, Ribosomal, 16S / genetics
- Terminology as Topic
Citations
This article has been cited 1 times.- Gonzalez-Pimentel JL, Dominguez-Moñino I, Jurado V, Laiz L, Caldeira AT, Saiz-Jimenez C. The Rare Actinobacterium Crossiella sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi. Microorganisms 2022 Aug 5;10(8).
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