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Home / Equine Research Bank / Anim Reprod Sci / Cryobiological determinants of frozen semen quality, with sp...
Animal reproduction science2008; 107(3-4); 276-292; doi: 10.1016/j.anireprosci.2008.05.001

Cryobiological determinants of frozen semen quality, with special reference to stallion.

Abstract: Success in cryopreserving stallion semen has been very variable. Several different freezing regimes have been published. However, because extenders and procedures used in each regime have differed, direct comparison of these techniques has been very difficult, and controlled studies comparing different techniques have not been reported. A number of different factors affect sperm cryosurvival. In this article we review briefly current cryopreservation procedures for stallion semen, and then in more detail cryobiological determinants of sperm function, and mechanisms of cryoinjury and cryoprotectant action. Specific attention is given to data relating to stallion sperm. The complexity of sperm cell biology is believed to be an important factor when developing improvements in stallion semen cryopreservation. It may be assumed that impairment of cell function resulting from cold and osmotic shock is a main source of stallion sperm sensitivity to conventional freezing procedures. Further physiological studies on stallion sperm are required to understand the mechanisms by which cryopreservation alters sperm function and influences selection of sperm with higher fertilizing potential. Such studies should focus especially on the processes involved in sperm volume regulation, sperm-oviduct interaction, capacitation and cellular signalling, and on the alterations in these processes caused by cryopreservation.
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Publication Date: 2008-05-09 PubMed ID: 18585878DOI: 10.1016/j.anireprosci.2008.05.001Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the challenges and complexities involved in preserving stallion sperm through freezing, discusses the factors affecting the viability of frozen sperm, and explores potential ways of improving the cryopreservation process.

Cryopreservation of Stallion Semen

  • The topic begins by pointing out the difficulties in achieving consistent success in stallion semen cryopreservation. There are many different freezing methods available, but they all employ varied extenders and procedures, making direct comparisons very challenging. The lack of controlled studies further complicates finding the most effective technique.

Factors Affecting Sperm Cryosurvival

  • This section emphasizes that the survival of sperm post-freezing depends on several factors. While the paper briefly outlines current cryopreservation procedures, it stresses the importance of understanding how cryobiological determinants of sperm function, and the mechanisms of cryoinjury and cryoprotectant action, influence sperm survival.
  • Particular attention is given to data concerning stallion sperm due to the complexity of its cell biology which might need special considerations when developing improvements in cryopreservation methods.

The Impact and Consequences of Cryopreservation

  • The researchers believe that the impairment of cell function resulting from cold and osmotic shock plays a significant part in the sensitivity of stallion sperm to conventional freezing procedures. Thus, further studies are encouraged to fully comprehend how cryopreservation alters sperm function and influences the selection of high fertile sperm.
  • Target areas for these studies should mainly include sperm volume regulation, interaction between sperm and oviduct, capacitation, cellular signalling, and how these processes get altered by cryopreservation.

Conclusion

  • The review concludes by suggesting the need for a detailed understanding of the complex biology of stallion sperm cells for effective cryopreservation. This could eventually lead to enhanced fertility rates and improved overall animal reproductive outcomes.

Cite This Article

APA
Sieme H, Harrison RA, Petrunkina AM. (2008). Cryobiological determinants of frozen semen quality, with special reference to stallion. Anim Reprod Sci, 107(3-4), 276-292. https://doi.org/10.1016/j.anireprosci.2008.05.001

Publication

Animal reproduction science
ISSN: 0378-4320
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 107
Issue: 3-4
Pages: 276-292

Researcher Affiliations

Sieme, H
  • Clinic for Horses, Reproductive Unit of Clinics, University of Veterinary Medicine, Hannover, Germany. harald.sieme@tiho-hannover.de
Harrison, R A P
    Petrunkina, A M

      MeSH Terms

      • Animals
      • Cryopreservation / methods
      • Cryopreservation / trends
      • Horses / physiology
      • Male
      • Quality Control
      • Semen Preservation / methods
      • Semen Preservation / trends
      • Semen Preservation / veterinary
      • Spermatozoa / cytology
      • Spermatozoa / physiology

      Citations

      This article has been cited 14 times.
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        doi: 10.3389/fvets.2022.1004683pubmed: 36337197google scholar: lookup
      2. Akhtar MF, Ma Q, Li Y, Chai W, Zhang Z, Li L, Wang C. Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology.. Animals (Basel) 2022 Sep 2;12(17).
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      3. Domain G, Ali Hassan H, Wydooghe E, Bogado Pascottini O, Johannisson A, Morrell JM, Niżański W, Van Soom A. Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs.. Animals (Basel) 2022 Mar 11;12(6).
        doi: 10.3390/ani12060714pubmed: 35327111google scholar: lookup
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        doi: 10.3390/antiox11020417pubmed: 35204299google scholar: lookup
      6. Ros-Santaella JL, Pintus E. Plant Extracts as Alternative Additives for Sperm Preservation.. Antioxidants (Basel) 2021 May 13;10(5).
        doi: 10.3390/antiox10050772pubmed: 34068069google scholar: lookup
      7. Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status.. Animals (Basel) 2021 Jan 3;11(1).
        doi: 10.3390/ani11010074pubmed: 33401609google scholar: lookup
      8. Ondřej Š, Jiří Š, Jan B, Pavla MP, Lucie T, Doležalová M, Petra F, Luděk S, Radko R. Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders.. Anim Reprod 2019 Oct 23;16(2):267-276.
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      9. Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm.. Biology (Basel) 2020 Jan 27;9(2).
        doi: 10.3390/biology9020022pubmed: 32012799google scholar: lookup
      10. Delgado-Bermúdez A, Noto F, Bonilla-Correal S, Garcia-Bonavila E, Catalán J, Papas M, Bonet S, Miró J, Yeste M. Cryotolerance of Stallion Spermatozoa Relies on Aquaglyceroporins rather than Orthodox Aquaporins.. Biology (Basel) 2019 Nov 12;8(4).
        doi: 10.3390/biology8040085pubmed: 31726707google scholar: lookup
      11. Alamaary MS, Haron AW, Ali M, Hiew MWH, Adamu L, Peter ID. Effects of four extenders on the quality of frozen semen in Arabian stallions.. Vet World 2019 Jan;12(1):34-40.
        doi: 10.14202/vetworld.2019.34-40pubmed: 30936651google scholar: lookup
      12. da Silva JC, Varela Junior AS, Caldas JS, Freitas Cda S, Botelho JG, Colares EP, Corcini CD. The effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae).. Fish Physiol Biochem 2016 Feb;42(1):93-102.
        doi: 10.1007/s10695-015-0120-3pubmed: 26342693google scholar: lookup
      13. Gil L, Galindo-Cardiel I, Malo C, González N, Alvarez C. Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm.. ISRN Vet Sci 2013;2013:280143.
        doi: 10.1155/2013/280143pubmed: 24416597google scholar: lookup
      14. Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA.. Acta Vet Scand 2012 Dec 5;54(1):72.
        doi: 10.1186/1751-0147-54-72pubmed: 23217215google scholar: lookup
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