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Home / Equine Research Bank / Virology / CTL from EIAV carrier horses with diverse MHC class I allele...
Virology2004; 327(1); 144-154; doi: 10.1016/j.virol.2004.06.035

CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins.

Abstract: Cytotoxic T lymphocytes (CTL) are important for controlling equine infectious anemia virus (EIAV). Because Gag matrix (MA) and capsid (CA) are the most frequently recognized proteins, the hypothesis that CTL from EIAV-infected horses with diverse MHC class I alleles recognize epitope clusters (EC) in these proteins was tested. Four EC were identified by CTL from 15 horses and 8 of these horses had diverse MHC class I alleles. Two of the eight had CTL to EC1, six to EC2, five to EC3, and four to EC4. Because EC2-4 were recognized by CTL from >50% of horses with diverse alleles, the hypothesis was accepted. EC1 and EC3 were the most conserved EC and these more conserved broadly recognized EC may be most useful for CTL induction, helping overcome MHC class I polymorphism and antigenic variation.
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Publication Date: 2004-08-26 PubMed ID: 15327905PubMed Central: PMC3342308DOI: 10.1016/j.virol.2004.06.035Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates how Cytotoxic T lymphocytes (CTL) from horses with diverse MHC class I alleles react to certain epitope clusters in two proteins often identified in equine infectious anemia virus (EIAV) infections. The study confirms the hypothesis that these CTL recognize the epitope clusters in the Gag matrix and capsid proteins.

Research Objectives and Hypothesis

  • The main objective of the research was to investigate how Cytotoxic T lymphocytes (CTL) from horses, infected with equine infectious anemia virus (EIAV), recognize epitope clusters (EC) within the frequently recognized proteins Gag matrix (MA) and capsid (CA).
  • The researchers hypothesized that CTL from horses with diverse MHC (major histocompatibility complex) class I alleles would recognize these EC in the MA and CA proteins.

Testing and Identification of Epitope Clusters

  • Through testing, four EC were identified by CTL from 15 different horses.
  • Out of these 15 horses, 8 horses had diverse MHC class I alleles.
  • The study found that two of the eight horses recognized EC1, six horses identified EC2, five horses detected EC3 and four horses noted EC4.

Findings and Hypothesis Confirmation

  • Observations that EC2, EC3, and EC4 were recognized by the CTL from more than 50% of the horses with diverse alleles served as a confirmation of the initial hypothesis.
  • The study further identified EC1 and EC3 clusters as the most conserved.

Conclusion and Implications

  • The findings suggest that these more conserved and broadly recognized EC (EC1 and EC3) could be used effectively for CTL induction in an effort to cope with the MHC class I polymorphism and antigenic variation which is common with virus infections.
  • The research thus provides valuable insights for potential strategies to efficiently control EIAV infections and similar viral diseases.

Cite This Article

APA
Chung C, Mealey RH, McGuire TC. (2004). CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins. Virology, 327(1), 144-154. https://doi.org/10.1016/j.virol.2004.06.035

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 327
Issue: 1
Pages: 144-154

Researcher Affiliations

Chung, Chungwon
  • Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99165-7040, USA.
Mealey, Robert H
    McGuire, Travis C

      MeSH Terms

      • Alleles
      • Amino Acid Sequence
      • Animals
      • Capsid Proteins / chemistry
      • Capsid Proteins / immunology
      • Carrier State / immunology
      • Carrier State / virology
      • Epitope Mapping
      • Epitopes, T-Lymphocyte / genetics
      • Equine Infectious Anemia / immunology
      • Gene Products, gag / chemistry
      • Gene Products, gag / immunology
      • Histocompatibility Antigens Class I / genetics
      • Horses
      • Infectious Anemia Virus, Equine / classification
      • Infectious Anemia Virus, Equine / genetics
      • Infectious Anemia Virus, Equine / immunology
      • Infectious Anemia Virus, Equine / pathogenicity
      • Molecular Sequence Data
      • T-Lymphocytes, Cytotoxic / immunology
      • Viral Matrix Proteins / chemistry
      • Viral Matrix Proteins / immunology

      Grant Funding

      • K08 AI001575 / NIAID NIH HHS
      • AI01575 / NIAID NIH HHS
      • AI24291 / NIAID NIH HHS
      • AI47660 / NIAID NIH HHS

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      Citations

      This article has been cited 10 times.
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