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Journal of anatomy2022; 241(5); 1211-1218; doi: 10.1111/joa.13719

Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport.

Abstract: Neurological disorders are prevalent in horses, but their study is challenging due to anatomic constraints and the large body size; very few host-specific in vitro models have been established to study these types of diseases, particularly from adult donor tissue. Here we report the generation of primary neuronal dorsal root ganglia (DRG) cultures from adult horses: the mixed, dissociated cultures, containing neurons and glial cells, remained viable for at least 90 days. Similar to DRG neurons in vivo, cultured neurons varied in size, and they developed long neurites. The mitochondrial movement was detected in cultured cells and was significantly slower in glial cells compared to DRG-derived neurons. In addition, mitochondria were more elongated in glial cells than those in neurons. Our culture model will be a useful tool to study the contribution of axonal transport defects to specific neurodegenerative diseases in horses as well as comparative studies aimed at evaluating species-specific differences in axonal transport and survival.
© 2022 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.
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Publication Date: 2022-06-21 PubMed ID: 35728923PubMed Central: PMC9558156DOI: 10.1111/joa.13719Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focused on creating a laboratory model using neurons from the dorsal root ganglia (DRG) of adult horses to investigate neurodegenerative diseases, specifically focusing on axonal transport mechanisms.

Objective of the Study

The research aimed to create a viable in-vitro model using mixed, dissociated primary dorsal root ganglia (DRG) cultures from adult horses. It sought to observe neuron and glial cell development and compare mitochondrial movement and structure between the two.

This model is intended for studying the impact of axonal transport defects in horse-related neurodegenerative diseases. It can also be used in comparative studies to understand species-specific differences in axonal transport and survival.

Methods Employed

  • The researchers generated dissociated primary DRG cultures from adult horses. These cultures included neurons and glial cells.
  • The viability of such cells was maintained over a period of 90 days or more.
  • They observed neuron development, particularly the growth of long neurites, akin to those seen in vivo.
  • The study also monitored and compared the movement of mitochondria between neurons and glial cells. Taken together, these methodologies produced in-depth insights into the behavior of cells in culture.

Key Findings

  • In cultures, just as in the in vivo setting, DRG neurons showed variations in size.
  • Mitochondrial movement was noted in cultured cells. Interestingly, glial cells featured significantly slower mitochondrial motion than DRG-derived neurons.
  • Moreover, mitochondria in glial cells were also more elongated than those in neurons.

Future Implications

The findings from this research herald new avenues for similar future studies. The novel culture model established here is expected to greatly contribute to the research field of horse-specific neurodegenerative diseases, elucidating the role of axonal transport abnormalities in these conditions.

Furthermore, it opens the way for comparative studies to examine and understand species-related differences in axonal transport and cell survival. This will provide greater depth to our knowledge of disease processes and potential therapies in horses specifically, and potentially other species as well.

Cite This Article

APA
Adalbert R, Cahalan S, Hopkins EL, Almuhanna A, Loreto A, Pór E, Körmöczy L, Perkins J, Coleman MP, Piercy RJ. (2022). Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport. J Anat, 241(5), 1211-1218. https://doi.org/10.1111/joa.13719

Publication

Journal of anatomy
ISSN: 1469-7580
NlmUniqueID: 0137162
Country: England
Language: English
Volume: 241
Issue: 5
Pages: 1211-1218

Researcher Affiliations

Adalbert, Robert
  • Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, UK.
  • Department of Anatomy, Histology, and Embryology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Cahalan, Stephen
  • Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, UK.
Hopkins, Eleanor L
  • John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
Almuhanna, Abdulaziz
  • Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, UK.
Loreto, Andrea
  • John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
  • Andrea Loreto, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Pór, Erzsébet
  • Department of Anatomy, Histology, and Embryology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Körmöczy, Laura
  • Department of Anatomy, Histology, and Embryology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Perkins, Justin
  • Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, UK.
Coleman, Michael P
  • John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
Piercy, Richard J
  • Comparative Neuromuscular Diseases Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, UK.

MeSH Terms

  • Animals
  • Axonal Transport
  • Cells, Cultured
  • Ganglia, Spinal
  • Horses
  • Neurites / physiology
  • Neurons

Grant Funding

  • UK Horserace Betting Levy Board

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Citations

This article has been cited 3 times.
  1. Lang Q, Schiavo G, Sleigh JN. In vivo imaging of axonal transport in peripheral nerves of rodent forelimbs. Neuronal Signal 2023 Mar;7(1):NS20220098.
    doi: 10.1042/NS20220098pubmed: 36743438google scholar: lookup
  2. Cahalan SD, Boehm I, Jones RA, Piercy RJ. Recognising the potential of large animals for modelling neuromuscular junction physiology and disease. J Anat 2022 Nov;241(5):1120-1132.
    doi: 10.1111/joa.13749pubmed: 36056593google scholar: lookup
  3. Sleigh JN. Editorial: Peripheral nerve anatomy in health and disease. J Anat 2022 Nov;241(5):1083-1088.
    doi: 10.1111/joa.13746pubmed: 36226698google scholar: lookup
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