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Home / Equine Research Bank / Equine Vet J / Cyclical endometrial steroid hormone receptor expression and...
Equine veterinary journal2000; 32(3); 228-232; doi: 10.2746/042516400776563554

Cyclical endometrial steroid hormone receptor expression and proliferation intensity in the mare.

Abstract: The aims of this study were to investigate the steroid hormone receptor expression and the proliferation intensity during the equine endometrial cycle by immunohistological methods, established for routine examination of formalin-fixed, paraplast-embedded specimens. Endometrial biopsy specimens were obtained during one cycle from 7 mares. In comparison with the blood steroid hormone levels the quantity and distribution of oestrogen (ER) and progesterone receptors (PR) and the proliferation marker Ki-67 antigen expression were investigated. Rising 17beta-oestradiol concentrations in preoestrus induce a synchronous expression of ER, PR and Ki-67 antigen in stromal cells. In the early dioestrus 17beta-oestradiol levels decrease and progesterone levels reach their maxima. This correlates with an intense proliferation activity and the highest hormone receptor expression in epithelial cells. In accordance to the morphological features of asynchronous glandular differentiation in fibrotic areas (endometrosis) their epithelial hormone receptor expression is out of phase.
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Publication Date: 2000-06-03 PubMed ID: 10836478DOI: 10.2746/042516400776563554Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aims to understand the expressions of steroid hormone receptors and the intensity of proliferation during the equine endometrial cycle using immunohistological methods on tissue samples obtained from seven mares.

Research Methodology

  • For the purpose of this study, endometrial biopsy samples were collected from seven mares throughout their reproductive cycle. This process was performed by using immunohistological methods that were established for formalin-fixed and paraplast-embedded specimens.
  • The researchers then observed and recorded the quantity and distribution of oestrogen receptors (ER), progesterone receptors (PR), and the proliferation marker Ki-67 antigen expression against the variations in blood steroid hormone levels.

Key Findings

  • The scientists noticed that an increase in 17beta-oestradiol concentrations in preoestrus induced synchronous expression of ER, PR, and Ki-67 antigen in stromal cells. This phase precedes ovulation in the mares’ reproductive cycle.
  • On the other hand, in the early phase of dioestrus, when 17beta-oestradiol levels decline and the levels of progesterone reach their maximum, there is intense proliferation activity and the highest hormone receptor expression in epithelial cells. This phase follows ovulation in the mares’ cycle.
  • The researchers also found that the hormonal receptor expression in the epithelial cells of fibrotic areas (endometrosis) is not synchronized with the morphological features of glandular differentiation. These fibrotic areas are seen as abnormalities in the endometrial lining and can affect a mare’s fertility.

Implications

  • The outcome of this research aids in the understanding of hormone receptor expression and cell proliferation during the equine endometrial cycle.
  • The insights may aid in improving the reproductive health of mares by shedding light on endometrial changes associated with the reproductive cycle. Treatment and therapeutic strategies for endometrial abnormalities could potentially be influenced by these findings.

Cite This Article

APA
Aupperle H, Ozgen SSchoon HA, Schoon D, Hoppen HO, Sieme H, Tannapfel A. (2000). Cyclical endometrial steroid hormone receptor expression and proliferation intensity in the mare. Equine Vet J, 32(3), 228-232. https://doi.org/10.2746/042516400776563554

Publication

Equine veterinary journal
ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 32
Issue: 3
Pages: 228-232

Researcher Affiliations

Aupperle, H
  • Institut für Veterinär-Pathologie, Universität Leipzig, An der Tierkliniken, Germany.
Ozgen SSchoon, H A
    Schoon, D
      Hoppen, H O
        Sieme, H
          Tannapfel, A

            MeSH Terms

            • Animals
            • Antibodies, Monoclonal
            • Biopsy / veterinary
            • Endometrium / cytology
            • Endometrium / metabolism
            • Estradiol / blood
            • Estrus / physiology
            • Female
            • Gene Expression Regulation
            • Horses / physiology
            • Immunohistochemistry
            • Ki-67 Antigen / analysis
            • Male
            • Progesterone / blood
            • Radioimmunoassay / veterinary
            • Receptors, Estradiol / genetics
            • Receptors, Estradiol / metabolism
            • Receptors, Progesterone / genetics
            • Receptors, Progesterone / metabolism

            Citations

            This article has been cited 16 times.
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