Decreased airway mucosal prostaglandin E2 production during airway obstruction in an animal model of asthma.
Abstract: Heaves is a respiratory disorder of horses and ponies characterized by bouts of acute airway obstruction and airway hyperresponsiveness. We measured prostaglandin E2 (PGE2) and 15-hydroxyeicosatetraenoic acid (15-HETE) production in vitro in tracheal epithelium obtained from six affected horses at the time of acute airway obstruction as compared with six matched control horses. Strips of epithelium and subepithelial tissue were prepared and stimulated with A23187, histamine, and bradykinin. The PGE2 and 15-HETE in media from strips was quantitated by radioimmunoassay. 15-HETE above the limits of accurate detection was found in epithelial strips of only two principal animals and in none of the control horses, and the amount of 15-HETE was not increased when strips were stimulated. Epithelial strips from affected horses tended to produce less PGE2 than did strips from control horses, and there was a significant correlation between epithelial PGE2 production and the time taken for affected animals to develop airway obstruction. Subepithelial tissue strips from control horses produced significantly more PGE2 in response to A23187 and bradykinin than did strips from affected horses. We conclude that equine tracheal epithelium is not a significant source of 15-HETE. Airway mucosal PGE2 production is reduced in horses with heaves, which suggests that a relative decrease in this bronchorelaxant substance may be a factor in the pathogenesis of this model of asthma.
Publication Date: 1992-09-01 PubMed ID: 1519833DOI: 10.1164/ajrccm/146.3.586Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research explores the respiratory disorder in horses and ponies called heaves, and its relation to decreased production of prostaglandin E2, a bronchorelaxant substance, during instances of acute airway obstruction. The report suggests that this decrease might play a part in the disorder’s development.
Objective of the Research
- The objective of this research was to understand the role of prostaglandin E2 (PGE2) in respiratory disorder known as ‘heaves’ in horses. The researchers wanted to investigate if decreased PGE2 production during acute airway obstruction is a factor in the pathogenesis of heaves.
Methods and Materials
- The researchers selected twelve horses for the study, six of which were affected by heaves, and the other six served as the control.
- They prepared strips of epithelium and subepithelial tissue from the trachea of these horses. These strips were then stimulated with A23187, histamine, and bradykinin.
- They quantified PGE2 and 15-hydroxyeicosatetraenoic acid (15-HETE) in media from these strips using the radioimmunoassay method.
Findings
- Just two principal horses from the affected group showed detectable levels of 15-HETE and none in the control group. Furthermore, stimulation didn’t increase the amount of 15-HETE, which implies that equine tracheal epithelium is not a significant source of 15-HETE.
- Affected horses’ epithelial strips produced less PGE2 than the control group. There was also a notable correlation between epithelial PGE2 production and the time it took for affected horses to develop airway obstruction.
- Subepithelial tissue from control horses produced significantly more PGE2 in response to A23187 and bradykinin compared to affected horses.
Conclusion
- The conclusion from this research indicates that the PGE2 production in the airway mucosa significantly decreases in horses affected by heaves. This suggests that the decreased production of this bronchorelaxant substance likely contributes to the pathogenesis of heaves.
Cite This Article
APA
Gray PR, Derksen FJ, Broadstone RV, Robinson NE, Peters-Golden M.
(1992).
Decreased airway mucosal prostaglandin E2 production during airway obstruction in an animal model of asthma.
Am Rev Respir Dis, 146(3), 586-591.
https://doi.org/10.1164/ajrccm/146.3.586 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824-1314.
MeSH Terms
- Airway Obstruction / metabolism
- Airway Obstruction / physiopathology
- Airway Obstruction / veterinary
- Animals
- Bradykinin / pharmacology
- Calcimycin / pharmacology
- Dinoprostone / analysis
- Dinoprostone / biosynthesis
- Epithelium / chemistry
- Epithelium / drug effects
- Epithelium / metabolism
- Histamine / pharmacology
- Horse Diseases / metabolism
- Horse Diseases / physiopathology
- Horses
- Hydroxyeicosatetraenoic Acids / analysis
- Hydroxyeicosatetraenoic Acids / biosynthesis
- Mucous Membrane / chemistry
- Mucous Membrane / drug effects
- Mucous Membrane / metabolism
- Radioimmunoassay
- Respiratory Function Tests / methods
- Respiratory Function Tests / statistics & numerical data
- Respiratory Function Tests / veterinary
- Trachea / chemistry
- Trachea / drug effects
- Trachea / metabolism
Citations
This article has been cited 3 times.- Torres R, Herrerias A, Serra-Pagès M, Roca-Ferrer J, Pujols L, Marco A, Picado C, de Mora F. An intranasal selective antisense oligonucleotide impairs lung cyclooxygenase-2 production and improves inflammation, but worsens airway function, in house dust mite sensitive mice. Respir Res 2008 Nov 12;9(1):72.
- Venugopalan CS, Beadle RE, Seahorn TL, Holmes EP. Responses of guinea-pig lung parenchymal strips to tracheobronchial lavage fluid from horses affected with summer pasture-associated obstructive pulmonary disease. Vet Res Commun 1998 Nov;22(7):493-503.
- Teixeira MM, Williams TJ, Hellewell PG. E-type prostaglandins enhance local oedema formation and neutrophil accumulation but suppress eosinophil accumulation in guinea-pig skin. Br J Pharmacol 1993 Sep;110(1):416-22.
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