Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1.
Abstract: VP22 is a major tegument protein of equine herpesvirus type 1 (EHV-1). In the present study, we examined functions of VP22 in EHV-1 replication by viral protein expression analyses in cells infected with the VP22-deficient virus. The expressions of several viral proteins in the cells infected with the VP22-deficient virus were lower than those in the cells infected with the parent virus. One of the weakly expressed proteins was identified as ICP4, which is a major regulatory protein encoded by an immediate early gene of EHV-1. A real-time PCR analysis showed that the mRNA expression of ICP4 was the same in cells infected with the parent and VP22-deficient viruses. Hence, VP22 appears to promote synthesis of ICP4 post-transcriptionally.
Publication Date: 2017-12-26 PubMed ID: 29279464PubMed Central: PMC5836769DOI: 10.1292/jvms.17-0380Google Scholar: Lookup
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Summary
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The research investigates the role of VP22, a protein in the equine herpesvirus type 1 (EHV-1), in the virus replication process, particularly in the production of the regulatory protein ICP4. They found that the absence of VP22 leads to lower expressions of several viral proteins, including ICP4, indicating it may influence ICP4 synthesis after transcription.
Roles and Functions of VP22 and ICP4 in EHV-1 Replication
- The research focuses on VP22, a major tegument protein in equine herpesvirus type 1 (EHV-1), which is a virus that infects horses.
- VP22’s functions in EHV-1 replication were investigated through viral protein expression analyses in cells infected with a version of the virus lacking VP22.
- ICP4 is identified as one of the weakly expressed proteins in the absence of VP22. It’s a crucial regulatory protein encoded by an immediate early gene of EHV-1. The immediate early genes are the first set of genes expressed after a virus enters a cell.
Impact of VP22 Deficiency on Viral Protein Expression
- The expressions of several viral proteins, including ICP4, were found to be lower in cells infected with the VP22-deficient virus compared to those infected with the standard EHV-1 virus.
- This suggests that VP22 plays a key role in promoting the production or expression of these proteins during the replication of the virus.
VP22 and Post-Transcriptional Synthesis of ICP4
- Real-time PCR analysis showed that the transcription of ICP4 into mRNA was the same in cells infected with both the VP22-deficient and the parent viruses. Transcription is the process of copying DNA into mRNA.
- Given that ICP4 protein synthesis is lower in the VP22-deficient cells even though mRNA expression is the same, it suggests that VP22 promotes the synthesis of ICP4 protein after the mRNA has been transcribed, which is known as post-transcriptional synthesis.
- This finding reveals a novel function of VP22 in the viral replication process, potentially providing a better understanding of the mechanisms behind EHV-1’s ability to infect and replicate within host cells.
Cite This Article
APA
Okada A, Suganuma S, Badr Y, Omatsu T, Mizutani T, Ohya K, Fukushi H.
(2017).
Decreased expression of the immediate early protein, ICP4, by deletion of the tegument protein VP22 of equine herpesvirus type 1.
J Vet Med Sci, 80(2), 311-315.
https://doi.org/10.1292/jvms.17-0380 Publication
Researcher Affiliations
- Department of Applied Veterinary Sciences, United Graduated School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Department of Applied Veterinary Sciences, United Graduated School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Department of Animal Medicine, Faculty of Veterinary Medicine, Damanhour University, El-Beheira, Egypt.
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
- Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
- Department of Applied Veterinary Sciences, United Graduated School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Department of Applied Veterinary Sciences, United Graduated School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
- Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
MeSH Terms
- Animals
- Dogs
- Gene Deletion
- Herpesvirus 1, Equid / genetics
- Herpesvirus 1, Equid / physiology
- Immediate-Early Proteins / biosynthesis
- Immediate-Early Proteins / genetics
- Madin Darby Canine Kidney Cells
- Transcription, Genetic
- Viral Structural Proteins / genetics
- Viral Structural Proteins / physiology
- Virus Replication / physiology
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Citations
This article has been cited 1 times.- Wu L, Cheng A, Wang M, Jia R, Yang Q, Wu Y, Zhu D, Zhao X, Chen S, Liu M, Zhang S, Ou X, Mao S, Gao Q, Sun D, Wen X, Liu Y, Yu Y, Zhang L, Tian B, Pan L, Chen X. Alphaherpesvirus Major Tegument Protein VP22: Its Precise Function in the Viral Life Cycle.. Front Microbiol 2020;11:1908.
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