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Home / Equine Research Bank / BMC Vet Res / Degenerative suspensory ligament desmitis as a systemic diso...
BMC veterinary research2006; 2; 12; doi: 10.1186/1746-6148-2-12

Degenerative suspensory ligament desmitis as a systemic disorder characterized by proteoglycan accumulation.

Abstract: Degenerative suspensory ligament desmitis (DSLD) is a debilitating disorder thought to be limited to suspensory ligaments of Peruvian Pasos, Peruvian Paso crosses, Arabians, American Saddlebreds, American Quarter Horses, Thoroughbreds, and some European breeds. It frequently leads to persistent, incurable lameness and need to euthanize affected horses. The pathogenesis remains unclear, though the disease appears to run in families. Treatment and prevention are empirical and supportive, and not effective in halting the progression of the disease. Presently, the presumptive diagnosis of DSLD is obtained from patient signalment and history, clinical examination, and ultrasonographic examination of clinically affected horses, and is confirmed at post mortem examination. Presently, there are no reliable methods of diagnosing DSLD in asymptomatic horses. The goal of this study was to characterize and define the disorder in terms of tissue involvement at the macroscopic and microscopic levels. Results: We examined tissues and organs from 28 affected horses (22 Peruvian Pasos, 6 horses of other breeds) and from 8 control horses. Histopathological examination revealed the presence of excessive amounts of proteoglycans in the following tissues removed from DSLD-affected horses: suspensory ligaments, superficial and deep digital flexor tendons, patellar and nuchal ligaments, cardiovascular system, and sclerae. Electron microscopy demonstrated changes in diameters of collagen fibrils in the tendon, and in smooth muscle cells of the media of the aorta compatible with increased cell permeability in DSLD-affected cells. Separation of tendon extracts by gel chromatography revealed the presence of additional proteoglycan(s) in extracts from affected, but not control extracts. Conclusions: This study demonstrates for the first time that DSLD, a disease process previously thought to be limited to the suspensory ligaments of the distal limbs of affected horses, is in fact a systemic disorder involving tissues and organs with significant connective tissue component. Abnormal accumulation of proteoglycans between collagen and elastic fibers rather than specific collagen fibril abnormalities is the most prominent histological feature of DSLD. Because of this observation and because of the involvement of many other tendons and ligaments beside the suspensory ligament, and of non-ligamentous tissue we, therefore, propose that equine systemic proteoglycan accumulation or ESPA rather than DSLD is a more appropriate name for this condition.
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Publication Date: 2006-04-12 PubMed ID: 16611357PubMed Central: PMC1459153DOI: 10.1186/1746-6148-2-12Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates a systemic disorder in horses known as Degenerative Suspensory Ligament Desmitis (DSLD), which was previously believed to be limited to certain suspensory ligaments, but now found to affect various organs and tissues. The disease causes excessive proteoglycan accumulation, and the researchers propose the name Equine Systemic Proteoglycan Accumulation (ESPA) for it.

Characterization of DSLD

  • The disease primarily affected Peruvian Pasos, Peruvian Paso crosses, Arabians, American Saddlebreds, American Quarter Horses, Thoroughbreds, and some European breeds. It caused incurable lameness, often resulting in euthanasia.
  • The disease is hereditary, but the exact pathogenesis remains unknown. The existing diagnosis is based on patient signalment and history, clinical and ultrasonographic examinations. Confirmation only occurs post mortem.

Goals and Methodology

  • The aim was to define DSLD in terms of tissue involvement at macroscopic and microscopic levels, to diagnose it in asymptomatic horses.
  • To investigate, they examined tissues and organs from 28 affected horses and 8 control horses, observing proteoglycan accumulation in ligaments, cardiovascular system, and sclerae.
  • Using electron microscopy, researchers observed changes in collagen fibrils and increased cell permeability in the tendon and aorta.
  • Tendon extracts were separated by gel chromatography, with additional proteoglycans found in affected extracts.

Findings and Conclusion

  • DSLD, previously thought to be localized within certain ligaments, was found to be a more systemic disorder, showing abnormal proteoglycan accumulation in various tissues and organs.
  • This study contradicts the prevailing belief that DSLD centers on specific collagen fibril abnormalities, instead pointing at proteoglycan accumulation as the key concern.
  • Giving the extent of the disease beyond the suspensory ligament, the researchers proposed renaming it to Equine Systemic Proteoglycan Accumulation (ESPA).

Cite This Article

APA
Halper J, Kim B, Khan A, Yoon JH, Mueller PO. (2006). Degenerative suspensory ligament desmitis as a systemic disorder characterized by proteoglycan accumulation. BMC Vet Res, 2, 12. https://doi.org/10.1186/1746-6148-2-12

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 2
Pages: 12

Researcher Affiliations

Halper, Jaroslava
  • Department of Pathology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA. jhalper@vet.uga.edu
Kim, Byoungjae
    Khan, Ahrar
      Yoon, Jung Hae
        Mueller, P O Eric

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          Citations

          This article has been cited 11 times.
          1. Momen M, Brauer K, Patterson MM, Sample SJ, Binversie EE, Davis BW, Cothran EG, Rosa GJM, Brounts SH, Muir P. Genetic architecture and polygenic risk score prediction of degenerative suspensory ligament desmitis (DSLD) in the Peruvian Horse.. Front Genet 2023;14:1201628.
            doi: 10.3389/fgene.2023.1201628pubmed: 37645058google scholar: lookup
          2. Roberts JH, Zhang J, David F, McLean A, Blumenshine K, Müller-Alander E, Halper J. Expression of genes with biomarker potential identified in skin from DSLD-affected horses increases with age.. PLoS One 2023;18(7):e0287740.
            doi: 10.1371/journal.pone.0287740pubmed: 37450486google scholar: lookup
          3. Momen M, Brounts SH, Binversie EE, Sample SJ, Rosa GJM, Davis BW, Muir P. Selection signature analyses and genome-wide association reveal genomic hotspot regions that reflect differences between breeds of horse with contrasting risk of degenerative suspensory ligament desmitis.. G3 (Bethesda) 2022 Sep 30;12(10).
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          4. Roberts JH, Halper J. Connective Tissue Disorders in Domestic Animals.. Adv Exp Med Biol 2021;1348:325-335.
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          5. Haythorn A, Young M, Stanton J, Zhang J, Mueller POE, Halper J. Differential gene expression in skin RNA of horses affected with degenerative suspensory ligament desmitis.. J Orthop Surg Res 2020 Oct 7;15(1):460.
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