Demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen.
Abstract: Antigenic relationship between purified equine infectious anemia (EIA) virus and spleen-derived antigen from EIA-infected horses was examined by immunodiffusion. Identical antigenicity of these two antigens has been proven because precipitation lines formed between the two antigens and EIA antiserum connected with each other. The results indicate that the antigenic substance derived from infected spleen is a component of EIA virus.
Publication Date: 1972-09-01 PubMed ID: 4629262PubMed Central: PMC422550DOI: 10.1128/iai.6.3.416-417.1972Google Scholar: Lookup
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- Journal Article
Summary
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The research sought to investigate the connection between the antigenic component derived from the spleen of horses infected with Equine Infectious Anemia (EIA) virus and the purified EIA virus itself. They found identical antigenic properties, suggesting that the antigen extracted from the infected spleen is a component of the EIA virus.
Objective of the Research
- The primary goal of this research was to determine the antigenic relationship between the spleen-derived antigen from horses infected with equine infectious anemia (EIA) virus and the purified EIA virus itself.
Methodology
- The researchers used immunodiffusion, a common laboratory technique for analysing antigens (substances that trigger an immune response), to ascertain whether the spleen-derived antigen and EIA virus shared the same antigenicity.
Findings
- The research found identical antigenicity between the spleen-derived antigen and the EIA virus. This was determined by observing that precipitation lines formed by the two antigens and EIA antiserum (a solution of antibodies used to test for an immune response) merged, indicating antigenic identity.
Significance of the Findings
- The findings from this study indicate that the antigenic substance derived from infected horse spleen is a component of the EIA virus. This discovery could be crucial for the future development of vaccines or treatments for EIA, as understanding the nature and functioning of antigens is key in designing effective immunological interventions.
Cite This Article
APA
Nakajima H, Norcross NL, Coggins L.
(1972).
Demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen.
Infect Immun, 6(3), 416-417.
https://doi.org/10.1128/iai.6.3.416-417.1972 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Antigens, Viral / analysis
- Chromatography, DEAE-Cellulose
- Equine Infectious Anemia / immunology
- Horses / immunology
- Immune Sera
- Immunodiffusion
- Spleen / immunology
References
This article includes 7 references
- Coggins L, Norcross NL. Immunodiffusion reaction in equine infectious anemia.. Cornell Vet 1970 Apr;60(2):330-5.
- Nakajima H, Ushimi C. Detection of precipitating antibody in equine infectious anemia by concentrated virus antigen.. Natl Inst Anim Health Q (Tokyo) 1972 Summer;12(2):47-53.
- Nakajima H, Tajima M, Tanaka S, Ushimi C. Physicochemical studies of equine infectionus anemia virus. 3. Purification and electron microscopic observation of the virus.. Arch Gesamte Virusforsch 1969;28(3):348-60.
- Coggins L, Norcross NL, Nusbaum SR. Diagnosis of equine infectious anemia by immunodiffusion test.. Am J Vet Res 1972 Jan;33(1):11-8.
- Nakajima H, Ushimi C. Immunodiffusion studies of purified equine infectious anemia virus.. Infect Immun 1971 Mar;3(3):373-7.
- Norcross NL, Coggins L. Characterization of an equine infectious anemia antigen extracted from infected horse spleen tissue.. Infect Immun 1971 Nov;4(5):528-31.
- Nakajima H, Kono Y, Ushimi C. Characterization of precipitating antibody in equine infectious anemia.. J Immunol 1971 Sep;107(3):889-94.
Citations
This article has been cited 4 times.- Malmquist WA, Barnett D, Becvar CS. Production of equine infectious anemia antigen in a persistently infected cell line. Arch Gesamte Virusforsch 1973;42(4):361-70.
- Nakajima H, Ushimi C, Fukunaga Y, Hirasawa K. Preparation of equine infectious anemia virus antigen for immunodiffusion test. Arch Gesamte Virusforsch 1973;42(4):339-45.
- Matheka HD, Coggins L, Shively JN, Norcross NL. Purification and characterization of equine infectious anemia virus. Arch Virol 1976;51(1-2):107-14.
- Archer BG, Crawford TB, McGuire TC, Frazier ME. RNA-dependent DNA polymerase associated with equine infectious anemia virus. J Virol 1977 Apr;22(1):16-22.
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