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Home / Equine Research Bank / Hum Cell / Derivation and induction of the differentiation of animal ES...
Human cell2006; 18(3); 135-141; doi: 10.1111/j.1749-0774.2005.tb00003.x

Derivation and induction of the differentiation of animal ES cells as well as human pluripotent stem cells derived from fetal membrane.

Abstract: We succeeded in the derivation and maintenance of pluripotent embryonic stem (ES) cells from equine and bovine blastocysts. These cells expressed markers that are characteristics of mouse ES cells, namely, alkaline phosphatase, stage-specific embryonic antigen 1, STAT 3 and Oct 4. We confirmed the pluripotential ability of these cells, which were able to undergo somatic differentiation in vitro to neural progenitors and to endothelial or hematopoietic lineages. We were able to use bovine ES cells as a source of nuclei for nuclear transfer and we generated cloned cattle with a higher frequency of pregnancies to term than has been achieved with somatic cells. On the other hand, we established human fetal membrane derived stem cell lines by the colonial cloning techniques using MEMalpha culture medium containing 10 ng/ml of EGF, 10 ng/ml of LIF and 10% fetal bovine serum (FBS). These cells appeared to maintain normal karyotype in vitro and expressed markers characteristics of stem cells. Furthermore, these cells contributed to the formation of chimeric murine embryoid bodies and gave rise to all three germ layers in vitro. Results from animal ES cells and human fetal membrane derived stem cells clearly demonstrate that these cells might be used for providing different types of cells for regenerative medicine as well as used for targeted genetic manipulation of the genome.
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Publication Date: 2006-10-07 PubMed ID: 17022145DOI: 10.1111/j.1749-0774.2005.tb00003.xGoogle Scholar: Lookup
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  • Non-U.S. Gov't
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper talks about the successful derivation and maintenance of pluripotent embryonic stem (ES) cells from horse and cow blastocysts and their ability to differentiate into different cell types. It also discusses the establishment of human fetal cell lines that could potentially be used for regenerative medicine or targeted genetic manipulation.

Derivation and Maintenance of Animal ES Cells

  • The researchers were successful in deriving and maintaining pluripotent embryonic stem (ES) cells from equine and bovine blastocysts, which are early-stage embryos.
  • These ES cells exhibited markers characteristic of mouse ES cells, including alkaline phosphatase, stage-specific embryonic antigen 1, STAT 3, and Oct 4.
  • The pluripotential ability of these cells was confirmed by their capacity to undergo somatic differentiation in vitro (in a lab setting) into neural progenitors and endothelial and hematopoietic lineages, meaning that they could potentially differentiate into a variety of cell types.
  • The researchers were even able to use bovine ES cells as a source of nuclei for nuclear transfer, which led to the creation of cloned cattle with a higher frequency of successful pregnancies to term than has been observed with somatic cells.

Establishment of Human Fetal Membrane Derived Stem Cell Lines

  • On the other hand, researchers constructed human fetal membrane-derived stem cell lines using colonial cloning techniques and a specific culture medium.
  • This type of stem cells also continued to maintain a normal karyotype in vitro, signifying healthy genetic makeup and stability.
  • They also expressed markers that are characteristic of stem cells, proving their stem cell nature.
  • Moreover, these cells made a contribution to chimeric murine embryoid body formation, a model system for studying early organogenesis and tissue development, and were capable of differentiating into all three primary germ layers (ectoderm, mesoderm, and endoderm) in vitro.

Implications and Applications

  • The study’s findings involving both the animal ES cells and the human fetal membrane-derived stem cells suggest that these cells could be a potential source for different cell types needed in regenerative medicine, which aims to repair or replace damaged or diseased cells, tissues, or organs.
  • Besides, they could also be used for targeted genetic manipulation of the genome, which could be critical in genetic research and therapies.

Cite This Article

APA
Saito S, Yokoyama K, Tamagawa T, Ishiwata I. (2006). Derivation and induction of the differentiation of animal ES cells as well as human pluripotent stem cells derived from fetal membrane. Hum Cell, 18(3), 135-141. https://doi.org/10.1111/j.1749-0774.2005.tb00003.x

Publication

Human cell
ISSN: 0914-7470
NlmUniqueID: 8912329
Country: Japan
Language: English
Volume: 18
Issue: 3
Pages: 135-141

Researcher Affiliations

Saito, Shigeo
  • Saito Laboratory of Cell Technology, 2095-20 Kataoka, Yaita, Tochigi 329-1571, Japan. saict1@maple.ocn.ne.jp
Yokoyama, Kazunari
    Tamagawa, Tomoharu
      Ishiwata, Isamu

        MeSH Terms

        • Animals
        • Cattle
        • Cell Differentiation
        • Embryonic Stem Cells / cytology
        • Extraembryonic Membranes / cytology
        • Horses
        • Humans
        • Mice
        • Pluripotent Stem Cells / cytology
        • Regenerative Medicine

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        Citations

        This article has been cited 6 times.
        1. Takahashi S, Ferdousi F, Zheng YW, Oda T, Isoda H. Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation.. Int J Mol Sci 2021 Apr 5;22(7).
          doi: 10.3390/ijms22073768pubmed: 33916494google scholar: lookup
        2. Gottipamula S, Sridhar KN. Large-scale Isolation, Expansion and Characterization of Human Amniotic Epithelial Cells.. Int J Stem Cells 2018 May 30;11(1):87-95.
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          doi: 10.3389/fnmol.2015.00035pubmed: 26283909google scholar: lookup
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        5. Baba T, Grebe R, Hasegawa T, Bhutto I, Merges C, McLeod DS, Lutty GA. Maturation of the fetal human choriocapillaris.. Invest Ophthalmol Vis Sci 2009 Jul;50(7):3503-11.
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          pubmed: 19119371
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