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Detection and molecular characterization of Actinomyces denticolens causing lymph node abscessation in horses.
Abstract: Abscessation of equine head lymph nodes can be caused by various bacteria, but Streptococcus equi subsp. equi is mainly involved. At our laboratory, samples of three unrelated horses with submandibular abscesses were found negative for S. equi, and further testing proved the presence of another genus. This raised the question for the exact identity of this pathogen and whether these isolates were epidemiologically related and it warranted further characterization with regards of virulence and resistance factors.
Copyright © 2023 van den Wollenberg, van Maanen, Buter, Janszen, Rey and van Engelen.
Publication Date: 2023-07-20 PubMed ID: 37546341PubMed Central: PMC10399742DOI: 10.3389/fvets.2023.1225528Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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Abscesses in the lymph nodes of horse heads can be caused by various bacteria. One primary culprit is Streptococcus equi subsp. equi. However, in three unrelated horses with submandibular abscesses at the research lab, samples tested negative for this bacteria.
Instead, they found another type of bacteria. This led to questions about the identity of this new pathogen, its origin, and its characteristics in terms of virulence and resistance.
Methods:
- Culture: The samples were grown in a controlled environment to observe the bacteria.
- Identification using MALDI-TOF MS: A type of mass spectrometry was used to identify the bacteria.
- MIC testing: This determined the minimum amount of antibiotic needed to inhibit bacterial growth.
- Whole genome sequencing (WGS): This provided a complete genetic makeup of the bacteria.
Results:
- Bacterial Identification: The bacteria were initially identified as A. denticolens in two of the three cases using the culture and MALDI-TOF MS method. Further confirmation of this identification for all three samples came from analyzing the WGS data, which was also supported by multilocus sequence typing (MLST).
- Genetic Findings: The three bacterial samples shared a 95% similarity in their nucleotide sequences. However, the significant number of single nucleotide polymorphisms (variations in DNA) indicated that the bacteria in the three samples were not clones of one another. This suggests that the three horses got the infection from different sources, not a single outbreak.
- Virulence and Resistance: Only four genes related to virulence (the ability to cause disease) were found in the bacteria. No known antibiotic resistance genes were detected, which was consistent with tests showing the bacteria were highly susceptible to antibiotics.
Conclusion:
A. denticolens is a potential cause of lymph node abscesses in horses, especially when traditional culprits like strangles are ruled out through lab tests. This discovery is significant as it adds to our knowledge of potential pathogens in horses. Moreover, the first draft genome of A. denticolens from horses was reported, adding to scientific knowledge about this bacteria’s genetics.
Cite This Article
APA
van den Wollenberg L, van Maanen C, Buter R, Janszen P, Rey F, van Engelen E.
(2023).
Detection and molecular characterization of Actinomyces denticolens causing lymph node abscessation in horses.
Front Vet Sci, 10.
https://doi.org/10.3389/fvets.2023.1225528 Publication
Researcher Affiliations
- Royal GD (Animal Health Service), Deventer, Netherlands.
- Royal GD (Animal Health Service), Deventer, Netherlands.
- Royal GD (Animal Health Service), Deventer, Netherlands.
- Equine Clinic De Raaphorst, Wassenaar, Netherlands.
- Veterinary Clinic Winsum, Equine Division, Winsum, Netherlands.
- Royal GD (Animal Health Service), Deventer, Netherlands.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
This article includes 25 references
- Albini S, Korczak BM, Abril C, Hüssy D, Limat S, Gerber V, Hermann M, Howald B, Miserez R. Mandibular lymphadenopathy caused by Actinomyces denticolens mimicking strangles in three horses.. Vet Rec 2008 Feb 2;162(5):158-9.
- Beck A, Baird JD, Slavić D. Submandibular lymph node abscess caused by Actinomyces denticolens in a horse in Ontario.. Can Vet J 2011 May;52(5):513-4.
- Feary DJ, Abraham S, Woolford L, Trott DJ. Identification of Actinomyces denticolens as a cause of a soft tissue abscess in a horse.. Aust Vet J 2013 Oct;91(10):416-417.
- Fielding CL, Magdesian KG, Morgan RA, Ruby RE, Sprayberry KA. Actinomyces species as a cause of abscesses in nine horses.. Vet Rec 2008 Jan 5;162(1):18-20.
- Murakami S, Kobayashi T, Sekigawa Y, Torii Y, Kanesaki Y, Ishige T, Yokoyama E, Ishiwata H, Hamada M, Tamura T. Actinomyces denticolens as a causative agent of actinomycosis in animals.. J Vet Med Sci 2018 Nov 9;80(11):1650-1656.
- Bailey GD, Love DN. Oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections.. Vet Microbiol 1991 Feb 15;26(4):367-79.
- Gajdács M, Urbán E, Terhes G. Microbiological and Clinical Aspects of Cervicofacial Actinomyces Infections: An Overview.. Dent J (Basel) 2019 Sep 1;7(3).
- Valour F, Sénéchal A, Dupieux C, Karsenty J, Lustig S, Breton P, Gleizal A, Boussel L, Laurent F, Braun E, Chidiac C, Ader F, Ferry T. Actinomycosis: etiology, clinical features, diagnosis, treatment, and management.. Infect Drug Resist 2014;7:183-97.
- Love DN, Vekselstein R, Collings S. The obligate and facultatively anaerobic bacterial flora of the normal feline gingival margin.. Vet Microbiol 1990 Apr;22(2-3):267-75.
- Putnins EE, Bowden GH. Antigenic relationships among oral Actinomyces isolates, Actinomyces naeslundii genospecies 1 and 2, Actinomyces howellii, Actinomyces denticolens, and Actinomyces slackii.. J Dent Res 1993 Oct;72(10):1374-85.
- Renvoise A, Raoult D, Roux V. Actinomyces timonensis sp. nov., isolated from a human clinical osteo-articular sample.. Int J Syst Evol Microbiol 2010 Jul;60(Pt 7):1516-1521.
- Madigan MT. Brock Biology of Microorganisms. 11th ed. Upper Saddle River, NJ: Prentice Hall; (2006).
- Connaway JW. Actinomycosis (Lump Jaw, Big Jaw, and Woodentongue). Cattle: University of Missouri, College of Agriculture, Agricultural Experiment Station; (1921).
- Smith BP. Large Animal Internal Medicine. 3 ed. St. Louis, MO: Mosby (2002).
- Dent VE, Williams RA. Actinomyces denticolens Dent & Williams sp. nov: a new species from the dental plaque of cattle.. J Appl Bacteriol 1984 Apr;56(2):183-92.
- Bohra D, Maherchandani S, Bahura C, Kashyap S. A comparative study of aerobic bacteria in upper respiratory tract of horses and donkeys. Vet Practition (2008) 9:50–1.
- Murakami S, Otaki M, Hayashi Y, Higuchi K, Kobayashi T, Torii Y, Yokoyama E, Azuma R. Actinomyces denticolens colonisation identified in equine tonsillar crypts.. Vet Rec Open 2016;3(1):e000161.
- Boyanova L, Kolarov R, Mateva L, Markovska R, Mitov I. Actinomycosis: a frequently forgotten disease.. Future Microbiol 2015;10(4):613-28.
- Hall V, Talbot PR, Stubbs SL, Duerden BI. Identification of clinical isolates of actinomyces species by amplified 16S ribosomal DNA restriction analysis.. J Clin Microbiol 2001 Oct;39(10):3555-62.
- Båverud V, Johansson SK, Aspan A. Real-time PCR for detection and differentiation of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus.. Vet Microbiol 2007 Oct 6;124(3-4):219-29.
- Robinson C, Steward KF, Potts N, Barker C, Hammond TA, Pierce K, Gunnarsson E, Svansson V, Slater J, Newton JR, Waller AS. Combining two serological assays optimises sensitivity and specificity for the identification of Streptococcus equi subsp. equi exposure.. Vet J 2013 Aug;197(2):188-91.
- Kanesaki Y, Ishige T, Sekigawa Y, Kobayashi T, Torii Y, Yokoyama E, Ishiwata H, Hamada M, Tamura T, Azuma R, Murakami S. Whole-Genome Sequences of Two Closely Related Bacteria, Actinomyces sp. Strain Chiba101 and Actinomyces denticolens DSM 20671(T).. Genome Announc 2017 Apr 6;5(14).
- Henssge U, Do T, Gilbert SC, Cox S, Clark D, Wickström C, Ligtenberg AJ, Radford DR, Beighton D. Application of MLST and pilus gene sequence comparisons to investigate the population structures of Actinomyces naeslundii and Actinomyces oris.. PLoS One 2011;6(6):e21430.
- Schürch AC, Arredondo-Alonso S, Willems RJL, Goering RV. Whole genome sequencing options for bacterial strain typing and epidemiologic analysis based on single nucleotide polymorphism versus gene-by-gene-based approaches.. Clin Microbiol Infect 2018 Apr;24(4):350-354.
- Steininger C, Willinger B. Resistance patterns in clinical isolates of pathogenic Actinomyces species.. J Antimicrob Chemother 2016 Feb;71(2):422-7.
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