Detection and molecular characterization of equine infectious anemia virus in Mongolian horses.
Abstract: The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94.0% identity with the third. These two independent Mongolian EIAV sequences were retained functional motifs, with no dramatic changes but some variability in the U5 region; they were clustered with genotypes from European countries but not with those from China, U.S.A., or Japan.
Publication Date: 2017-10-11 PubMed ID: 29021424PubMed Central: PMC5709569DOI: 10.1292/jvms.17-0202Google Scholar: Lookup
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- Journal Article
Summary
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The study was aimed at exploring and describing the genetic properties and prevalence of the Equine Infectious Anemia Virus (EIAV) in horses in Mongolia.
Objective and Methodology
- The research aimed to uncover the true prevalence of EIAV and its genetic characteristics within horses in Mongolia, an area known for the disease but not well understood.
- A total of 776 horse serum samples were collected from four different provinces in Mongolia. The samples were then tested using an agar gel immunodiffusion technique to detect the presence of EIAV.
- Genomic DNA was extracted from the 11 samples that tested positive and was subjected further to a nested PCR assay. The aim of this was to amplify and identify certain segments of the EIAV genome.
Results and Findings
- Out of these 11 samples, only three tested positive from the nested PCR assay, indicating the presence of EIAV. These three samples underwent sequencing analysis for the virus’s long termination repeat and tat gene.
- Two of the three samples contained identical sequences, exhibiting a 94.0% identity with the third sample. This suggests a significant similarity and potentially common source or origin of infection.
- The genetic sequences of the Mongolian EIAV carried functional motifs, with minor variations noted particularly in the U5 region of the virus genome. This region is often associated with regulatory functions influencing the virus’s ability to infect and replicate.
- The study interestingly found that the Mongolian sequences grouped with genotypes from European countries, but not those identified from China, the United States, or Japan. This finding suggests differing viral strains or variations regionally.
Implications of the Study
- The findings of the study contribute to our understanding of the genetic variation and distribution of EIAV within Mongolia and globally.
- Knowledge of these variations can be crucial in the development and improvement of diagnostic tools and treatment strategies.
- The data gathered also hints at potential regional variations in EIAV strains, furthering our comprehension of the evolution and spread of the disease.
Cite This Article
APA
Sharav T, Konnai S, Ochirkhuu N, Ts EO, Mekata H, Sakoda Y, Umemura T, Murata S, Chultemdorj T, Ohashi K.
(2017).
Detection and molecular characterization of equine infectious anemia virus in Mongolian horses.
J Vet Med Sci, 79(11), 1884-1888.
https://doi.org/10.1292/jvms.17-0202 Publication
Researcher Affiliations
- Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia.
- Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan.
- Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia.
- Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia.
- Organization for Promotion of Tenure Track, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan.
- Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan.
- Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan.
- Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia.
- Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan.
- Department of Infectious Diseases and Public Health, School of Veterinary Medicine, Mongolian University of Life Science, Khan-Uul district, Zaisan 17042, Ulaanbaatar, Mongolia.
- Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan.
MeSH Terms
- Animals
- DNA, Viral
- Equine Infectious Anemia / epidemiology
- Equine Infectious Anemia / virology
- Genetic Variation
- Genome, Viral
- Horses
- Infectious Anemia Virus, Equine / genetics
- Infectious Anemia Virus, Equine / isolation & purification
- Mongolia / epidemiology
- Polymerase Chain Reaction / veterinary
- Sequence Analysis, DNA
- Seroepidemiologic Studies
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Citations
This article has been cited 2 times.- Lupulovic D, Savić S, Gaudaire D, Berthet N, Grgić Ž, Matović K, Deshiere A, Hans A. Identification and genetic characterization of equine infectious anemia virus in Western Balkans.. BMC Vet Res 2021 Apr 15;17(1):168.
- Alnaeem AA, Hemida MG. Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016.. Vet World 2019 May;12(5):719-723.
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