Detection and nucleotide sequencing of a DNA-packaging protein gene of equine gammaherpesviruses.
Abstract: In previous studies, novel putative viral pathogens designated that asinine herpesvirus 4 (AsHV4) and asinine herpesvirus 5 (AsHV5) were associated with fatal interstitial pneumonia in donkeys (Equus asinus). Nucleotide sequence analysis of a portion of the DNA polymerase gene identified these putative pathogens as herpesviruses and possibly as members of the Gammaherpesvirinae subfamily. Although similar to equine herpesvirus 2 (EHV2) and equine herpesvirus 5 (EHV5), sequence diversity was observed among the detected viruses. In this study, novel sequence is reported for a DNA-packaging protein gene of EHV5 plus AsHV4, AsHV5, and a newly described putative pathogen herein designated asinine herpesvirus 6 (AsHV6). Phylogenetic analysis of these sequences suggested that the equine gammaherpesviruses may form a separate clade within the Gammaherpesvirinae subfamily. Based on the sequence of EHV2 and the novel sequences reported in this study, a PCR assay was developed to detect equine gammaherpesviruses. Products of the predicted size were produced after amplification of DNA from EHV2, EHV5, AsHV4, AsHV5, and AsHV6. This nonnested assay was shown to consistently amplify approximately 10 genomic copies of EHV2. Amplification products were not produced from DNA template of other alpha- and gammaherpesviruses. Because the role of gammaherpesviruses has not been well defined in equine disease, it is envisioned that a single, sensitive PCR assay to detect these potential pathogens will facilitate further assessment of their role in disease.
Publication Date: 2004-02-21 PubMed ID: 14974850DOI: 10.1177/104063870401600112Google Scholar: Lookup
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- Journal Article
Summary
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This is a study that aims to identify, sequence, and develop a method for detecting a particular gene found in a subfamily of herpesviruses that affect equines. The researchers have uncovered a new virulent strain within this subfamily, and the aim is to gain a better understanding of its role in causing disease.
Objective of the Study
- The purpose of this research was to identify and sequence a DNA-packaging protein gene within equine gammaherpesviruses, a subfamily of viruses responsible for diseases in equines, such as horses and donkeys.
- Additionally, the study aimed to develop a polymerase chain reaction (PCR) assay for detecting these equine gammaherpesviruses to facilitate the exploration of their role in disease.
Methodology and Findings
- In previous studies, potential pathogens, asinine herpesvirus 4 (AsHV4) and asinine herpesvirus 5 (AsHV5), were linked to fatal pneumonia in donkeys. Nucleotide sequencing of a portion of their DNA polymerase gene confirmed them as herpesviruses and possibly members of the Gammaherpesvirinae subfamily.
- This study revealed a novel sequence for a DNA-packaging protein gene of EHV5, AsHV4, AsHV5, and a newly identified potential pathogen named asinine herpesvirus 6 (AsHV6).
- A phylogenetic analysis of these sequences suggested that equine gammaherpesviruses might form a separate clade or subgroup within the Gammaherpesvirinae subfamily.
- Using the novel sequences and the sequence of EHV2, researchers developed a PCR assay to detect equine gammaherpesviruses. This methodology could consistently amplify approximately 10 genomic copies of EHV2.
Practical Implications
- The research provides a method for detecting equine gammaherpesviruses, which can aid further studies in assessing the role of these potential pathogens in causing diseases in equines.
- The research could offer insights that may lead to the development of therapeutic interventions against these potential pathogens, especially if their role in disease causation is established.
Cite This Article
APA
Kleiboeker SB, Turnquist SE, Johnson PJ, Kreeger JM.
(2004).
Detection and nucleotide sequencing of a DNA-packaging protein gene of equine gammaherpesviruses.
J Vet Diagn Invest, 16(1), 67-74.
https://doi.org/10.1177/104063870401600112 Publication
Researcher Affiliations
- Veterinary Medical Diagnostic Laboratory, Department of Veterinary Pathobiology, University of Missouri, College of Veterinary Medicine, Columbia, MO 65211, USA.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Viral / chemistry
- DNA, Viral / genetics
- DNA-Directed DNA Polymerase / chemistry
- DNA-Directed DNA Polymerase / genetics
- Endodeoxyribonucleases / genetics
- Gammaherpesvirinae / classification
- Gammaherpesvirinae / enzymology
- Gammaherpesvirinae / genetics
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Horse Diseases / virology
- Horses
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction / veterinary
- Sequence Alignment
Citations
This article has been cited 4 times.- Mira F, Canuti M, Di Bella S, Puleio R, Lavazza A, Lelli D, Vicari D, Purpari G, Cannella V, Chiaramonte G, Schirò G, Castronovo C, Guercio A. Detection and Molecular Characterization of Two Gammaherpesviruses from Pantesco Breed Donkeys during an Outbreak of Mild Respiratory Disease.. Viruses 2021 Aug 2;13(8).
- Jerele S, Davis E, Mapes S, Pusterla N, Navas González FJ, Iglesias Pastrana C, Abdelfattah EM, McLean A. Survey of Serum Amyloid A and Bacterial and Viral Frequency Using qPCR Levels in Recently Captured Feral Donkeys from Death Valley National Park (California).. Animals (Basel) 2020 Jun 23;10(6).
- Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
- Pozo F, Juste J, Vázquez-Morón S, Aznar-López C, Ibáñez C, Garin I, Aihartza J, Casas I, Tenorio A, Echevarría JE. Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution.. PLoS One 2016;11(12):e0169153.
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