Detection of African horsesickness viral antigens in tissues by immunofluorescence.
Abstract: The fluorescent antibody reaction was studied in tissues of ponies infected with African horsesickness virus (AHSV). Lung, spleen, lymph node, liver, skeletal muscle, intestine, stomach, nerve ganglion and kidney were sectioned and stained by the direct fluorescent antibody technique (FA). Fluorescence was demonstrated only in the spleen and could be inhibited by using unconjugated antiserum.
Publication Date: 1972-04-01 PubMed ID: 4259931PubMed Central: PMC1319637
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- Journal Article
Summary
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This research paper describes an investigation into the presence of African horsesickness virus (AHSV) in different tissues of infected ponies, using fluorescence-based antibody detection methods. The researchers found that the virus was identifiable only in the spleens of the infected ponies.
Methodology Used in the Research
The authors conducted their investigations using a method named the fluorescent antibody technique (FA). This process involves a set of steps:
- Tissue samples were collected from various parts of the infected ponies, namely, the lung, spleen, lymph node, liver, skeletal muscle, intestine, stomach, nerve ganglion, and kidney.
- These samples were sectioned, a process that involves cutting samples into thin slices that can be observed under a microscope.
- Each section was then exposed to a specific antibody that has been labeled with a fluorescent dye. When these antibodies bind to the AHSV antigens (if present) on the sections, they will emit light under fluorescent conditions, signaling the presence of the virus.
Results and Findings
Key findings of the research included:
- Fluorescence indicative of the presence of AHSV was found exclusively in the spleens of the infected ponies. No other tissues demonstrated such fluorescence when tested using the fluorescent antibody technique.
- This implies that the AHSV antigens were not detectable in any of the other tissues tested from the ponies infected with the virus.
- The fluorescence observed in the spleen was able to be inhibited by using unconjugated antiserum, further confirming the presence of AHSV in that organ.
Significance of the Research
These results are significant because they demonstrate a potential method for reliable detection of AHSV in the spleen tissue of infected ponies. Moving forward, a rapid and accurate diagnosis of AHSV can contribute to controlling the spread of the disease and may help in preventing large-scale infections. While the absence of fluorescence in the other tissue does not conclusively demonstrate that the virus is absent from these tissues, it does highlight the need for further investigations and refinements of detection methods.
Cite This Article
APA
Tessler J.
(1972).
Detection of African horsesickness viral antigens in tissues by immunofluorescence.
Can J Comp Med, 36(2), 167-169.
Publication
Researcher Affiliations
MeSH Terms
- African Horse Sickness / immunology
- Animals
- Antigens / analysis
- Antigens, Viral / analysis
- Fluorescent Antibody Technique / veterinary
- Horses
- Immune Sera
- Reoviridae / immunology
- Spleen / immunology
- Virus Cultivation
References
This article includes 11 references
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- COFFIN DL, LIU C. Studies of canine distemper infection by means of fluorescein-labeled antibody. II. The pathology and diagnosis of the naturally occurring disease in dogs and the antigenic nature of the inclusion body.. Virology 1957 Feb;3(1):132-45.
- MIRCHAMSY H, TASLIMI H. VISUALIZATION OF HORSE SICKNESS VIRUS BY THE FLUORESCENT ANTIBODY TECHNIQUE.. Immunology 1964 May;7(3):213-6.
- GOLDMAN M. Staining toxoplasma gondii with fluorescein-labelled antibody. II. A new serologic test for antibodies to Toxoplasma based upon inhibition of specific staining.. J Exp Med 1957 Jun 1;105(6):557-73.
- Ozawa Y. Studies on the replication of African horse-sickness virus in two different cell line cultures.. Arch Gesamte Virusforsch 1967;21(2):155-69.
- Mengeling WL, Pirtle EC, Torrey JP. Identification of Hog Cholera Viral Antigen by Immunofluorescence. Application as a Diagnostic and Assay Method.. Can J Comp Med Vet Sci 1963 Oct;27(10):249-52.
- Fernelius AL. Noncytopathogenic Bovine Viral Diarrhea Viruses Detected and Titrated by Immunofluorescence.. Can J Comp Med Vet Sci 1964 May;28(5):121-6.
- ALBRECHT P, BLASKOVIC D, STYK B, KOLLER M. COURSE OF A2 INFLUENZA IN INTRANASALLY INFECTED MICE EXAMINED BY THE FLUORESCENT ANTIBODY TECHNIQUE.. Acta Virol 1963 Sep;7:405-13.
- GOLDWASSER RA, KISSLING RE. Fluorescent antibody staining of street and fixed rabies virus antigens.. Proc Soc Exp Biol Med 1958 Jun;98(2):219-23.
- Boulanger P, Bannister GL, Greig AS, Gray DP, Ruckerbauer GM, Willis NG. African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence.. Can J Comp Med Vet Sci 1967 Jan;31(1):16-23.
Citations
This article has been cited 1 times.- Tinarwo M, Dennis SJ, Hitzeroth II, Meyers AE, Rybicki EP, Mbewana S. Development of an African horse sickness VP6 DIVA diagnostic ELISA. Virol J 2025 Aug 12;22(1):276.
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