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Home / Equine Research Bank / Appl Environ Microbiol / Detection of Ehrlichia risticii, the agent of Potomac horse ...
Applied and environmental microbiology1998; 64(8); 2888-2893; doi: 10.1128/AEM.64.8.2888-2893.1998

Detection of Ehrlichia risticii, the agent of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.) from northern California.

Abstract: Ehrlichia DNA was identified by nested PCR in operculate snails (Pleuroceridae: Juga spp.) collected from stream water in a northern California pasture in which Potomac horse fever (PHF) is enzootic. Sequencing of PCR-amplified DNA from a suite of genes (the 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes) indicated that the source organism closely resembled Ehrlichia risticii, the causative agent of PHF. The minimum percentage of Juga spp. harboring the organism in the population studied was 3.5% (2 of 57 snails). No ehrlichia DNA was found in tissues of 123 lymnaeid, physid, and planorbid snails collected at the same site. These data suggest that pleurocerid stream snails may play a role in the life cycle of E. risticii in northern California.
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Publication Date: 1998-08-04 PubMed ID: 9687446PubMed Central: PMC106788DOI: 10.1128/AEM.64.8.2888-2893.1998Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study found a specific strain of bacteria, known as Ehrlichia risticii – the cause of Potomac horse fever – in a particular type of snail found in a northern California pasture, suggesting that these snails may contribute to the spread of this disease.

Research Methodology

  • The research involved collecting operculate snails (Pleuroceridae: Juga spp.) from a northern California pasture where an outbreak of Potomac horse fever (PHF) was known to be endemic.
  • The researchers used a technique known as nested PCR to identify traces of Ehrlichia DNA within the snails.
  • The researchers also examined the DNA sequences of a set of genes which includes 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes. This helped to confirm that the DNA traces found in the snails were consistent with Ehrlichia risticii, the bacteria that causes PHF.

Key Findings

  • The researchers found Ehrlichia risticii within a minimal percentage (3.5%) of the Juga spp. snails sampled (2 out of the 57 studied).
  • Additionally, the researchers studied 123 other snails (lymnaeid, physid, and planorbid) found in the same location, but none of these others showed any trace of the Ehrlichia DNA.

Implications of the Findings

  • The discovery of Ehrlichia risticii within the Juga spp. snails implies that these snails could have a role in the life cycle of this bacterium, and hence the spread of PHF, at least in the specific context of northern California.
  • This adds to the body of knowledge on the spread of PHF, potentially aiding future efforts in controlling and preventing outbreaks of this disease.

Cite This Article

APA
Barlough JE, Reubel GH, Madigan JE, Vredevoe LK, Miller PE, Rikihisa Y. (1998). Detection of Ehrlichia risticii, the agent of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.) from northern California. Appl Environ Microbiol, 64(8), 2888-2893. https://doi.org/10.1128/AEM.64.8.2888-2893.1998

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 64
Issue: 8
Pages: 2888-2893

Researcher Affiliations

Barlough, J E
  • Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA.
Reubel, G H
    Madigan, J E
      Vredevoe, L K
        Miller, P E
          Rikihisa, Y

            MeSH Terms

            • Amino Acid Sequence
            • Animals
            • Antigens, Bacterial / genetics
            • Bacterial Proteins / genetics
            • Base Sequence
            • California
            • Chaperonins / genetics
            • DNA, Ribosomal / analysis
            • Ehrlichia / genetics
            • Ehrlichia / isolation & purification
            • Ehrlichiosis / transmission
            • Ehrlichiosis / veterinary
            • Fresh Water
            • Heat-Shock Proteins / genetics
            • Horse Diseases / microbiology
            • Horse Diseases / transmission
            • Horses
            • Molecular Sequence Data
            • Operon
            • Phylogeny
            • Polymerase Chain Reaction
            • RNA, Ribosomal, 16S / genetics
            • Snails / microbiology

            References

            This article includes 45 references
            1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool.. J Mol Biol 1990 Oct 5;215(3):403-10.
              pubmed: 2231712doi: 10.1016/s0022-2836(05)80360-2google scholar: lookup
            2. Barlough JE, Madigan JE, DeRock E, Bigornia L. Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus).. Vet Parasitol 1996 Jun;63(3-4):319-29.
              pubmed: 8966998doi: 10.1016/0304-4017(95)00904-3google scholar: lookup
            3. Barlough JE, Rikihisa Y, Madigan JE. Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses.. Vet Parasitol 1997 Mar;68(4):367-73.
              pubmed: 9106958doi: 10.1016/s0304-4017(96)01083-7google scholar: lookup
            4. BENNINGTON E, PRATT I. The life history of the salmonpoisoning fluke, Nanophyetus salmincola (Chapin).. J Parasitol 1960 Feb;46:91-100.
              pubmed: 13798970
            5. Burch J B. North American freshwater snails. Hamburg, Mich: Malacological Publications; 1989.
            6. BURNS WC. Six virgulate xiphidiocercariae from Oregon, including redescriptions of Allassogonoporus vespertilionis and Acanthatrium oregonense.. J Parasitol 1961 Dec;47:919-25.
              pubmed: 13874996
            7. BURNS WC. Penetration and development of Allassogonoporus vespertilionis and Acanthatrium oregonense (Trematoda: Lecithodendriidae) cercariae in caddis fly larvae.. J Parasitol 1961 Dec;47:927-32.
              pubmed: 13874994
            8. Chaichanasiriwithaya W, Rikihisa Y, Yamamoto S, Reed S, Crawford TB, Perryman LE, Palmer GH. Antigenic, morphologic, and molecular characterization of new Ehrlichia risticii isolates.. J Clin Microbiol 1994 Dec;32(12):3026-33.
              pmc: PMC264219pubmed: 7533780doi: 10.1128/jcm.32.12.3026-3033.1994google scholar: lookup
            9. CORDY DR, GORHAM JR. The pathology and etiology of salmon disease.. Am J Pathol 1950 Jul;26(4):617-37.
              pmc: PMC1942931pubmed: 15419288
            10. Dillon R T. Karyotypic evolution in pleurocerid snails. II. Pleurocera, Goniobasis, and Juga.. Malacologia 1991;33:339–344.
            11. Dumler JS, Bakken JS. Ehrlichial diseases of humans: emerging tick-borne infections.. Clin Infect Dis 1995 May;20(5):1102-10.
              pubmed: 7619983doi: 10.1093/clinids/20.5.1102google scholar: lookup
            12. Dutta SK, Shankarappa B, Mattingly-Napier BL. Molecular cloning and analysis of recombinant major antigens of Ehrlichia risticii.. Infect Immun 1991 Mar;59(3):1162-9.
              pmc: PMC258382pubmed: 1997417doi: 10.1128/iai.59.3.1162-1169.1991google scholar: lookup
            13. Dutta SK, Shankarappa B, Thaker SR, Mattingly-Napier BL. DNA restriction endonuclease cleavage pattern and protein antigen profile of Ehrlichia risticii.. Vet Microbiol 1990 Oct;25(1):29-38.
              pubmed: 2247934doi: 10.1016/0378-1135(90)90090-igoogle scholar: lookup
            14. Felsenstein J. PHYLIP—phylogeny inference package.. Cladistics 1989;5:164–166.
            15. FUKUDA T, SASAHARA T, KITAO T. [Studies on the causative agent of Hyuga fever. 10. The vector].. Nihon Densenbyo Gakkai Zasshi 1962 Sep;36:235-41.
              pubmed: 13960183
            16. Fukuda T, Yamamoto S. Neorickettsia-like organism isolated from metacercaria of a fluke, Stellantchasmus falcatus.. Jpn J Med Sci Biol 1981 Apr;34(2):103-7.
              pubmed: 7311106doi: 10.7883/yoken1952.34.103google scholar: lookup
            17. Gupta RS. Evolution of the chaperonin families (Hsp60, Hsp10 and Tcp-1) of proteins and the origin of eukaryotic cells.. Mol Microbiol 1995 Jan;15(1):1-11.
              pubmed: 7752884doi: 10.1111/j.1365-2958.1995.tb02216.xgoogle scholar: lookup
            18. Hahn NE, Fletcher M, Rice RM, Kocan KM, Hansen JW, Hair JA, Barker RW, Perry BD. Attempted transmission of Ehrlichia risticii, causative agent of Potomac horse fever, by the ticks, Dermacentor variabilis, Rhipicephalus sanguineus, Ixodes scapularis and Amblyomma americanum.. Exp Appl Acarol 1990 Jan;8(1-2):41-50.
              pubmed: 2307070doi: 10.1007/bf01193380google scholar: lookup
            19. Kahler S. Transmission is unsolved mystery of equine monocytic ehrlichiosis.. J Am Vet Med Assoc 1989 Jun 15;194(12):1681-5, 1687.
              pubmed: 2753790
            20. Knowles R C, Anderson C W, Shipley W D, Whitlock R H, Perry B D, Davidson J P. Acute equine diarrhea syndrome (AEDS): a preliminary report. Proceedings of the 29th Annual Meeting of the American Association of Equine Practitioners, Lexington, Ky 1983; pp. 353–357.
            21. Madigan J E, Barlough J E, Rikihisa Y, Wen B, Miller P E, Sampson T J. Identification of an enzootic diarrhea (“Shasta River crud”) in northern California as Potomac horse fever.. J Equine Vet Sci 1997;17:270–272.
            22. Madigan J E, Cohen M, Stabbe M T, True R G, Wohlford L A. Serologic evidence of Potomac horse fever (Ehrlichia risticii) in three California horses with enterocolitis and fever.. Calif Vet 1987;41:8–10.
            23. Madigan JE, Rikihisa Y, Palmer JE, DeRock E, Mott J. Evidence for a high rate of false-positive results with the indirect fluorescent antibody test for Ehrlichia risticii antibody in horses.. J Am Vet Med Assoc 1995 Dec 1;207(11):1448-53.
              pubmed: 7493874
            24. Millemann RE, Knapp SE. Biology of Nanophyetus salmincola and "salmon poisoning" disease.. Adv Parasitol 1970;8:1-41.
              pubmed: 4934030
            25. Mott J, Rikihisa Y, Zhang Y, Reed SM, Yu CY. Comparison of PCR and culture to the indirect fluorescent-antibody test for diagnosis of Potomac horse fever.. J Clin Microbiol 1997 Sep;35(9):2215-9.
              pmc: PMC229942pubmed: 9276390doi: 10.1128/jcm.35.9.2215-2219.1997google scholar: lookup
            26. Olsen GJ, Woese CR. Ribosomal RNA: a key to phylogeny.. FASEB J 1993 Jan;7(1):113-23.
              pubmed: 8422957doi: 10.1096/fasebj.7.1.8422957google scholar: lookup
            27. Palmer JE. Potomac horse fever.. Vet Clin North Am Equine Pract 1993 Aug;9(2):399-410.
              pubmed: 8358652doi: 10.1016/s0749-0739(17)30406-6google scholar: lookup
            28. Palmer JE, Benson CE. Studies on oral transmission of Potomac horse fever.. J Vet Intern Med 1994 Mar-Apr;8(2):87-92.
              pubmed: 8046681doi: 10.1111/j.1939-1676.1994.tb03203.xgoogle scholar: lookup
            29. Perry BD, Rikihisa Y, Saunders GK. Intradermal transmission of Potomac horse fever.. Vet Rec 1985 Mar 2;116(9):246-7.
              pubmed: 4002597doi: 10.1136/vr.116.9.246google scholar: lookup
            30. Perry BD, Schmidtmann ET, Rice RM, Hansen JW, Fletcher M, Turner EC, Robl MG, Hahn NE. Epidemiology of Potomac horse fever: an investigation into the possible role of non-equine mammals.. Vet Rec 1989 Jul 22;125(4):83-6.
              pubmed: 2773237doi: 10.1136/vr.125.4.83google scholar: lookup
            31. Pretzman C, Ralph D, Stothard DR, Fuerst PA, Rikihisa Y. 16S rRNA gene sequence of Neorickettsia helminthoeca and its phylogenetic alignment with members of the genus Ehrlichia.. Int J Syst Bacteriol 1995 Apr;45(2):207-11.
              pubmed: 7537055doi: 10.1099/00207713-45-2-207google scholar: lookup
            32. Reubel GH, Barlough JE, Madigan JE. Production and characterization of Ehrlichia risticii, the agent of Potomac horse fever, from snails (Pleuroceridae: Juga spp.) in aquarium culture and genetic comparison to equine strains.. J Clin Microbiol 1998 Jun;36(6):1501-11.
              pmc: PMC104868pubmed: 9620368doi: 10.1128/jcm.36.6.1501-1511.1998google scholar: lookup
            33. Rikihisa Y. Cross-reacting antigens between Neorickettsia helminthoeca and Ehrlichia species, shown by immunofluorescence and Western immunoblotting.. J Clin Microbiol 1991 Sep;29(9):2024-9.
              pmc: PMC270252pubmed: 1774330doi: 10.1128/jcm.29.9.2024-2029.1991google scholar: lookup
            34. Rikihisa Y, Perry BD. Causative ehrlichial organisms in Potomac horse fever.. Infect Immun 1985 Sep;49(3):513-7.
              pmc: PMC261191pubmed: 4030092doi: 10.1128/iai.49.3.513-517.1985google scholar: lookup
            35. Rikihisa Y, Perry BD, Cordes DO. Ultrastructural study of ehrlichial organisms in the large colons of ponies infected with Potomac horse fever.. Infect Immun 1985 Sep;49(3):505-12.
              pmc: PMC261190pubmed: 4030091doi: 10.1128/iai.49.3.505-512.1985google scholar: lookup
            36. Rikihisa Y, Pretzman CI, Johnson GC, Reed SM, Yamamoto S, Andrews F. Clinical, histopathological, and immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii challenge.. Infect Immun 1988 Nov;56(11):2960-6.
              pmc: PMC259677pubmed: 3169993doi: 10.1128/iai.56.11.2960-2966.1988google scholar: lookup
            37. Schmidtmann ET, Robl MG, Carroll JF. Attempted transmission of Ehrlichia risticii by field-captured Dermacentor variabilis (Acari: Ixodidae).. Am J Vet Res 1986 Nov;47(11):2393-5.
              pubmed: 3789501
            38. Simms B T, Donham C R, Shaw J N, McCapes A M. Salmon poisoning.. J Am Vet Med Assoc 1931;78:181–195.
            39. Sumner JW, Nicholson WL, Massung RF. PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species.. J Clin Microbiol 1997 Aug;35(8):2087-92.
              pmc: PMC229908pubmed: 9230387doi: 10.1128/jcm.35.8.2087-2092.1997google scholar: lookup
            40. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.. Nucleic Acids Res 1994 Nov 11;22(22):4673-80.
              pmc: PMC308517pubmed: 7984417doi: 10.1093/nar/22.22.4673google scholar: lookup
            41. Vemulapalli R, Biswas B, Dutta SK. Pathogenic, immunologic, and molecular differences between two Ehrlichia risticii strains.. J Clin Microbiol 1995 Nov;33(11):2987-93.
              pmc: PMC228620pubmed: 8576359doi: 10.1128/jcm.33.11.2987-2993.1995google scholar: lookup
            42. Wen B, Rikihisa Y, Fuerst PA, Chaichanasiriwithaya W. Diversity of 16S rRNA genes of new Ehrlichia strains isolated from horses with clinical signs of Potomac horse fever.. Int J Syst Bacteriol 1995 Apr;45(2):315-8.
              pubmed: 7537065doi: 10.1099/00207713-45-2-315google scholar: lookup
            43. Wen B, Rikihisa Y, Yamamoto S, Kawabata N, Fuerst PA. Characterization of the SF agent, an Ehrlichia sp. isolated from the fluke Stellantchasmus falcatus, by 16S rRNA base sequence, serological, and morphological analyses.. Int J Syst Bacteriol 1996 Jan;46(1):149-54.
              pubmed: 8573488doi: 10.1099/00207713-46-1-149google scholar: lookup
            44. Wilson KH, Blitchington RB, Greene RC. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.. J Clin Microbiol 1990 Sep;28(9):1942-6.
              pmc: PMC268083pubmed: 2095137doi: 10.1128/jcm.28.9.1942-1946.1990google scholar: lookup
            45. Woese CR. Bacterial evolution.. Microbiol Rev 1987 Jun;51(2):221-71.
              pmc: PMC373105pubmed: 2439888doi: 10.1128/mr.51.2.221-271.1987google scholar: lookup

            Citations

            This article has been cited 11 times.
            1. Perles L, Barreto WTG, Macedo GC, Herrera HM, Machado RZ, André MR. Neorickettsia sp. in coatis (Nasua nasua) in Brazil.. Rev Bras Parasitol Vet 2023;32(3):e006623.
              doi: 10.1590/S1984-29612023042pubmed: 37466626google scholar: lookup
            2. Arroyo LG, Moore A, Bedford S, Gomez DE, Teymournejad O, Xiong Q, Budachetri K, Bekebrede H, Rikihisa Y, Baird JD. Potomac horse fever in Ontario: Clinical, geographic, and diagnostic aspects.. Can Vet J 2021 Jun;62(6):622-628.
              pubmed: 34219771
            3. Teymournejad O, Lin M, Bekebrede H, Kamr A, Toribio RE, Arroyo LG, Baird JD, Rikihisa Y. Isolation and Molecular Analysis of a Novel Neorickettsia Species That Causes Potomac Horse Fever.. mBio 2020 Feb 25;11(1).
              doi: 10.1128/mBio.03429-19pubmed: 32098825google scholar: lookup
            4. Greiman SE, Tkach VV, Vaughan JA. Transmission rates of the bacterial endosymbiont, Neorickettsia risticii, during the asexual reproduction phase of its digenean host, Plagiorchis elegans, within naturally infected lymnaeid snails.. Parasit Vectors 2013 Oct 22;6:303.
              doi: 10.1186/1756-3305-6-303pubmed: 24383453google scholar: lookup
            5. Baird JD, Arroyo LG. Historical aspects of Potomac horse fever in Ontario (1924-2010).. Can Vet J 2013 Jun;54(6):565-72.
              pubmed: 24155447
            6. Gibson KE, Pastenkos G, Moesta S, Rikihisa Y. Neorickettsia risticii surface-exposed proteins: proteomics identification, recognition by naturally-infected horses, and strain variations.. Vet Res 2011 Jun 2;42(1):71.
              doi: 10.1186/1297-9716-42-71pubmed: 21635728google scholar: lookup
            7. Rikihisa Y, Zhang C, Kanter M, Cheng Z, Ohashi N, Fukuda T. Analysis of p51, groESL, and the major antigen P51 in various species of Neorickettsia, an obligatory intracellular bacterium that infects trematodes and mammals.. J Clin Microbiol 2004 Aug;42(8):3823-6.
              doi: 10.1128/JCM.42.8.3823-3826.2004pubmed: 15297539google scholar: lookup
            8. Mott J, Muramatsu Y, Seaton E, Martin C, Reed S, Rikihisa Y. Molecular analysis of Neorickettsia risticii in adult aquatic insects in Pennsylvania, in horses infected by ingestion of insects, and isolated in cell culture.. J Clin Microbiol 2002 Feb;40(2):690-3.
              doi: 10.1128/JCM.40.2.690-693.2002pubmed: 11825999google scholar: lookup
            9. Kanter M, Mott J, Ohashi N, Fried B, Reed S, Lin YC, Rikihisa Y. Analysis of 16S rRNA and 51-kilodalton antigen gene and transmission in mice of Ehrlichia risticii in virgulate trematodes from Elimia livescens snails in Ohio.. J Clin Microbiol 2000 Sep;38(9):3349-58.
              doi: 10.1128/JCM.38.9.3349-3358.2000pubmed: 10970382google scholar: lookup
            10. Pusterla N, Madigan JE, Chae JS, DeRock E, Johnson E, Pusterla JB. Helminthic transmission and isolation of Ehrlichia risticii, the causative agent of Potomac horse fever, by using trematode stages from freshwater stream snails.. J Clin Microbiol 2000 Mar;38(3):1293-7.
              doi: 10.1128/JCM.38.3.1293-1297.2000pubmed: 10699046google scholar: lookup
            11. Reubel GH, Barlough JE, Madigan JE. Production and characterization of Ehrlichia risticii, the agent of Potomac horse fever, from snails (Pleuroceridae: Juga spp.) in aquarium culture and genetic comparison to equine strains.. J Clin Microbiol 1998 Jun;36(6):1501-11.
              doi: 10.1128/JCM.36.6.1501-1511.1998pubmed: 9620368google scholar: lookup
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