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Home / Equine Research Bank / Braz J Microbiol / Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper...
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019; 50(3); 875-878; doi: 10.1007/s42770-019-00100-7

Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil.

Abstract: Equid gammaherpesvirus 2 (EHV-2) and 5 (EHV-5) are members of the Herpesviridae family and have been reported in horse populations worldwide. This study aimed to evaluate the presence of herpesvirus DNA in the upper respiratory tract of horses. Twenty-six nasal swabs were collected from asymptomatic adult horses of two different horse farms (A, n = 18; B, n = 8), both located in Southern Brazil. The EHV-1, EHV-2, EHV-4, and EHV-5 DNA analyses were performed using nested PCR assays targeting the glycoprotein B gene. Four (15.3%) and 12 (46.1%) of the 26 nasal swab samples were positive for the EHV-2 and EHV-5, respectively. Four (15.3%) horses were detected with both viruses simultaneously. DNA of EHV-2 and EHV-5 in both single and mixed infections was identified in horses from both herds. All swab samples were negative for EHV-1 and EHV-4. This study reports the first detection of EHV-2 and EHV-5 in the upper respiratory tracts of horses in Brazil. The high detection rate of EHV-2 and EHV-5 in asymptomatic adult horses demonstrates that these gammaherpesviruses are circulating in Brazil.
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Publication Date: 2019-06-11 PubMed ID: 31187445PubMed Central: PMC6863341DOI: 10.1007/s42770-019-00100-7Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research discovers the presence of Equid gammaherpesvirus 2 (EHV-2) and 5 (EHV-5) DNA in the upper respiratory tract of asymptomatic horses in southern Brazil, indicating that these gammaherpesviruses exist in the region.

Objective and Methodology of the Study

  • The study focuses on detecting the presence of DNA from two specific equid gammaherpesviruses: EHV-2 and EHV-5. These are members of the herpes virus family found in horse populations globally.
  • Twenty-six nasal swabs were collected from asymptomatic adult horses in two different horse farms located in Southern Brazil.
  • To analyze the presence of EHV-1, EHV-2, EHV-4, and EHV-5, a nested PCR assay was conducted targeting the glycoprotein B gene.

Findings

  • In the 26 nasal swab samples, 15.3% (4 samples) were positive for EHV-2 and 46.1% (12 samples) for EHV-5.
  • The presence of both EHV-2 and EHV-5 was found simultaneously in 15.3% (4) of the horses.
  • DNA of EHV-2 and EHV-5 was identified in horses from both farms in single and mixed infections.
  • All swab samples resulted negative for EHV-1 and EHV-4.

Significance of the Results

  • The findings of this research indicate the first detection of EHV-2 and EHV-5 DNA in the upper respiratory tracts of horses in Brazil.
  • The occurrence of EHV-2 and EHV-5 DNA in asymptomatic horses implies that these gammaherpesviruses are circulating in the horse population in Brazil, even if no visible symptoms are present.
  • This has potential health implications for the horse population and hints at a need for further research and possible monitoring or treatment strategies.

Cite This Article

APA
Dall Agnol AM, Beuttemmuller EA, Pilz D, Leme RA, Saporiti V, Headley SA, Alfieri AF, Alfieri AA. (2019). Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil. Braz J Microbiol, 50(3), 875-878. https://doi.org/10.1007/s42770-019-00100-7

Publication

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
NlmUniqueID: 101095924
Country: Brazil
Language: English
Volume: 50
Issue: 3
Pages: 875-878

Researcher Affiliations

Dall Agnol, Alais Maria
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
  • Center for Agroveterinary Sciences, Universidade do Estado de Santa Catarina, Lages, Santa Catarina, Brazil.
Beuttemmuller, Edsel Alves
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
  • Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
Pilz, Daniela
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
Leme, Raquel Arruda
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
  • Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
Saporiti, Viviane
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
Headley, Selwyn Arlington
  • Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.
  • Multi-User Animal Health Laboratory, Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.
Alfieri, Alice Fernandes
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
  • Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil.
Alfieri, Amauri Alcindo
  • Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil. alfieri@uel.br.
  • Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Rodovia Celso Garcia Cid - Campus Universitário, Universidade Estadual de Londrina, PO Box 10011, Londrina, PR, CEP 86057-970, Brazil. alfieri@uel.br.

MeSH Terms

  • Animals
  • Asymptomatic Diseases
  • Brazil
  • DNA, Viral / genetics
  • Female
  • Gammaherpesvirinae / classification
  • Gammaherpesvirinae / genetics
  • Gammaherpesvirinae / isolation & purification
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Horse Diseases / virology
  • Horses
  • Male
  • Nose / virology

Conflict of Interest Statement

The authors declare that they have no conflict of interest.

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Citations

This article has been cited 4 times.
  1. de Almeida Campos AC, Cicolo S, de Oliveira CM, Molina CV, Navas-Suárez PE, Poltronieri Dos Santos T, da Silveira VB, Barbosa CM, Baccarin RYA, Durigon EL, Góes LGB, Belli CB, Oliveira DBL. Potential outbreak by herpesvirus in equines: detection, clinical, and genetic analysis of equid gammaherpesvirus 2 (EHV-2). Braz J Microbiol 2023 Jun;54(2):1137-1143.
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  2. Finger MA, Biava JS, Dornbusch PT, Perotta JH, Ullmann LS, Serpa PBDS, Kmetiuk LB, Dos Santos AP, Biondo AW, Leutenegger C, Filho IRB. Molecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil. Vet World 2022 Nov;15(11):2597-2602.
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  3. Scheurer L, Bachofen C, Hardmeier I, Lechmann J, Schoster A. Prevalence of Nasal Shedding of Equid Gammaherpesviruses in Healthy Swiss Horses. Viruses 2021 Aug 25;13(9).
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  4. Cooper CJ, Arroyo LG, Hammermueller JD, Botts MM, Pearl DL, Wootton SK, Lillie BN. Molecular prevalence of equine alphaherpesvirus-1 shedding in healthy broodmares in Ontario. Can J Vet Res 2026 Jan;90(1):16-24.
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