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Journal of clinical microbiology1999; 37(7); 2285-2290; doi: 10.1128/JCM.37.7.2285-2290.1999

Detection of equine antibodies to babesia caballi by recombinant B. caballi rhoptry-associated protein 1 in a competitive-inhibition enzyme-linked immunosorbent assay.

Abstract: A competitive-inhibition enzyme-linked immunosorbent assay (cELISA) was developed for detection of equine antibodies specific for Babesia caballi. The assay used recombinant B. caballi rhoptry-associated protein 1 (RAP-1) and monoclonal antibody (MAb) 79/17.18.5, which is reactive with a peptide epitope of a native 60-kDa B. caballi antigen. The gene encoding the recombinant antigen was sequenced, and database analysis revealed that the gene product is a rhoptry-associated protein. Cloning and expression of a truncated copy of the gene demonstrated that MAb 79/17.18.5 reacts with the C-terminal repeat region of the protein. The cELISA was used to evaluate 302 equine serum samples previously tested for antibodies to B. caballi by a standardized complement fixation test (CFT). The results of cELISA and CFT were 73% concordant. Seventy-two of the 77 serum samples with discordant results were CFT negative and cELISA positive. Further evaluation of the serum samples with discordant results by indirect immunofluorescence assay (IFA) demonstrated that at a serum dilution of 1:200, 48 of the CFT-negative and cELISA-positive serum samples contained antibodies reactive with B. caballi RAP-1. Four of five CFT-positive and cELISA-negative serum samples contained antibodies reactive with B. caballi when they were tested by IFA. These data indicate that following infection with B. caballi, horses consistently produce antibody to the RAP-1 epitope defined by MAb 79/17.18.5, and when used in the cELISA format, recombinant RAP-1 is a useful antigen for the serologic detection of anti-B. caballi antibodies.
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Publication Date: 1999-06-12 PubMed ID: 10364599PubMed Central: PMC85139DOI: 10.1128/JCM.37.7.2285-2290.1999Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research deals with the development of a new testing method for Babesia caballi antibodies in horses, using a competitive-inhibition enzyme-linked immunosorbent assay (cELISA) technique, which proved to be more accurate than the standardized method due to the use of recombinant B. caballi rhoptry-associated protein 1 (RAP-1) and a specific monoclonal antibody.

Research Method

  • The study began with the development of a cELISA for the detection of equine antibodies specifically for Babesia caballi – a parasite that affects horses.
  • This process utilized a recombinant (synthetically created) protein from B. caballi known as rhoptry-associated protein 1 (RAP-1), along with monoclonal antibody (MAb) 79/17.18.5, known to react with a peptide epitope of a native 60-kDA B. caballi antigen.
  • The gene encoding the recombinant antigen was sequenced and database analysis revealed that the gene product is a rhoptry-associated protein.

Cloning and Expression of the cELISA

  • The researchers cloned and expressed a truncated copy of the gene, which showed that MAb 79/17.18.5 reacts with the C-terminal repeat region of the protein.
  • To evaluate the cELISA, they used 302 equine serum samples previously tested for anti-B. caballi antibodies using a standardized complement fixation test (CFT).
  • They found that the results of the cELISA and the CFT were in agreement in 73% of the cases.

Validation of cELISA

  • When further tested using indirect immunofluorescence assay (IFA) with discordant samples, 48 CFT-negative and cELISA-positive samples contained antibodies reactive with B. caballi RAP-1.
  • Moreover, 4 out of 5 CFT-positive and cELISA-negative samples contained antibodies reactive with B. caballi when tested by IFA.
  • This shows that horses infected with B. caballi consistently produce antibodies to the RAP-1 epitope defined by MAb 79/17.18.5.
  • This demonstrates that when used in a cELISA format, recombinant RAP-1 is a beneficial antigen for detecting anti-B. caballi antibodies, showing an enhanced sensitivity and specificity compared to the standard CFT test.

Cite This Article

APA
Kappmeyer LS, Perryman LE, Hines SA, Baszler TV, Katz JB, Hennager SG, Knowles DP. (1999). Detection of equine antibodies to babesia caballi by recombinant B. caballi rhoptry-associated protein 1 in a competitive-inhibition enzyme-linked immunosorbent assay. J Clin Microbiol, 37(7), 2285-2290. https://doi.org/10.1128/JCM.37.7.2285-2290.1999

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 37
Issue: 7
Pages: 2285-2290

Researcher Affiliations

Kappmeyer, L S
  • Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030, USA.
Perryman, L E
    Hines, S A
      Baszler, T V
        Katz, J B
          Hennager, S G
            Knowles, D P

              MeSH Terms

              • Amino Acid Sequence
              • Animals
              • Antibodies, Monoclonal
              • Antibodies, Protozoan / blood
              • Antigens, Protozoan / genetics
              • Antigens, Protozoan / immunology
              • Babesia / genetics
              • Babesia / immunology
              • Babesiosis / diagnosis
              • Babesiosis / immunology
              • Enzyme-Linked Immunosorbent Assay
              • Epitopes / analysis
              • Horses
              • Molecular Sequence Data
              • Protozoan Proteins / chemistry
              • Protozoan Proteins / genetics
              • Protozoan Proteins / immunology
              • Recombinant Proteins / immunology
              • Repetitive Sequences, Amino Acid

              References

              This article includes 16 references
              1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool.. J Mol Biol 1990 Oct 5;215(3):403-10.
                pubmed: 2231712doi: 10.1016/s0022-2836(05)80360-2google scholar: lookup
              2. Baszler TV, Knowles DP, Dubey JP, Gay JM, Mathison BA, McElwain TF. Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay.. J Clin Microbiol 1996 Jun;34(6):1423-8.
                pmc: PMC229036pubmed: 8735092doi: 10.1128/jcm.34.6.1423-1428.1996google scholar: lookup
              3. Böse R, Daemen K. Demonstration of the humoral immune response of horses to Babesia caballi by western blotting.. Int J Parasitol 1992 Aug;22(5):627-30.
                pubmed: 1399247doi: 10.1016/0020-7519(92)90011-9google scholar: lookup
              4. Böse R. Polyclonal antibody characterization of Babesia caballi antigens.. Int J Parasitol 1994 Jul;24(4):511-7.
                pubmed: 7521861doi: 10.1016/0020-7519(94)90142-2google scholar: lookup
              5. Frerichs WM, Holbrook AA, Johnson AJ. Equine piroplasmosis: complement-fixation titers of horses infected with Babesia caballi.. Am J Vet Res 1969 May;30(5):697-702.
                pubmed: 5813666
              6. Holman PJ, Frerichs WM, Chieves L, Wagner GG. Culture confirmation of the carrier status of Babesia caballi-infected horses.. J Clin Microbiol 1993 Mar;31(3):698-701.
                pmc: PMC262846pubmed: 8458966doi: 10.1128/jcm.31.3.698-701.1993google scholar: lookup
              7. Kappmeyer LS, Perryman LE, Knowles DP Jr. A Babesia equi gene encodes a surface protein with homology to Theileria species.. Mol Biochem Parasitol 1993 Nov;62(1):121-4.
                pubmed: 8114813doi: 10.1016/0166-6851(93)90185-zgoogle scholar: lookup
              8. Knowles DP Jr, Perryman LE, Goff WL, Miller CD, Harrington RD, Gorham JR. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites.. Infect Immun 1991 Jul;59(7):2412-7.
                pmc: PMC258026pubmed: 1711016doi: 10.1128/iai.59.7.2412-2417.1991google scholar: lookup
              9. Knowles DP Jr, Kappmeyer LS, Stiller D, Hennager SG, Perryman LE. Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi.. J Clin Microbiol 1992 Dec;30(12):3122-6.
                pmc: PMC270599pubmed: 1280648doi: 10.1128/jcm.30.12.3122-3126.1992google scholar: lookup
              10. Knowles D, Torioni de Echaide S, Palmer G, McGuire T, Stiller D, McElwain T. Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle.. J Clin Microbiol 1996 Sep;34(9):2225-30.
                pmc: PMC229221pubmed: 8862589doi: 10.1128/jcm.34.9.2225-2230.1996google scholar: lookup
              11. Li H, Shen DT, Knowles DP, Gorham JR, Crawford TB. Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus.. J Clin Microbiol 1994 Jul;32(7):1674-9.
                pmc: PMC263759pubmed: 7523438doi: 10.1128/jcm.32.7.1674-1679.1994google scholar: lookup
              12. McElwain TF, Perryman LE, Davis WC, McGuire TC. Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites.. J Immunol 1987 Apr 1;138(7):2298-304.
                pubmed: 2435796
              13. McGuire TC, Van Hoosier GL Jr, Henson JB. The complement-fixation reaction in eguine infectious anemia: demonstration of inhibition by IgG (T).. J Immunol 1971 Dec;107(6):1738-44.
                pubmed: 5000991
              14. Suarez CE, Palmer GH, Hötzel I, McElwain TF. Structure, sequence, and transcriptional analysis of the Babesia bovis rap-1 multigene locus.. Mol Biochem Parasitol 1998 Jun 1;93(2):215-24.
                pubmed: 9662706doi: 10.1016/s0166-6851(98)00032-2google scholar: lookup
              15. Torioni de Echaide S, Knowles DP, McGuire TC, Palmer GH, Suarez CE, McElwain TF. Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5.. J Clin Microbiol 1998 Mar;36(3):777-82.
                pmc: PMC104624pubmed: 9508311doi: 10.1128/jcm.36.3.777-782.1998google scholar: lookup
              16. Yokoyama WM. Production of monoclonal antibodies. Current protocols in immunology 1994; pp. 2.2.1–2.5.17.

              Citations

              This article has been cited 36 times.
              1. Sevinc F, Zhou M, Cao S, Ceylan O, Ulucesme MC, Ozubek S, Aktas M, Xuan X. Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep. Front Cell Infect Microbiol 2023;13:1238369.
                doi: 10.3389/fcimb.2023.1238369pubmed: 37662014google scholar: lookup
              2. El-Sayed SAE, Rizk MA, Baghdadi HB, Ringo AE, Sambuu G, Nugraha AB, Igarashi I. Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA. PLoS One 2023;18(4):e0284535.
                doi: 10.1371/journal.pone.0284535pubmed: 37058508google scholar: lookup
              3. Yang G, Zhou B, Chen K, Hu Z, Guo W, Wang X, Du C. Diagnostic Performance of Competitive ELISA and Western Blot Methods for the Detection of Antibodies against Theileria equi and Babesia caballi. Microorganisms 2022 Dec 21;11(1).
                doi: 10.3390/microorganisms11010021pubmed: 36677312google scholar: lookup
              4. Graham H, van Kalsbeek P, van der Goot J, Koene MGJ. Low seroprevalence of equine piroplasmosis in horses exported from the Netherlands between 2015 and 2021. Front Vet Sci 2022;9:954046.
                doi: 10.3389/fvets.2022.954046pubmed: 36299627google scholar: lookup
              5. Salinas-Estrella E, Ueti MW, Lobanov VA, Castillo-Payró E, Lizcano-Mata A, Badilla C, Martínez-Ibáñez F, Mosqueda J. Serological and molecular detection of Babesia caballi and Theileria equi in Mexico: A prospective study. PLoS One 2022;17(3):e0264998.
                doi: 10.1371/journal.pone.0264998pubmed: 35259206google scholar: lookup
              6. Yang G, Chen K, Guo W, Hu Z, Qi T, Liu D, Wang Y, Du C, Wang X. Development of a Test Card Based on Colloidal Gold Immunochromatographic Strips for Rapid Detection of Antibodies against Theileria equi and Babesia caballi. Microbiol Spectr 2022 Feb 23;10(1):e0241121.
                doi: 10.1128/spectrum.02411-21pubmed: 35196786google scholar: lookup
              7. Bastos RG, Thekkiniath J, Ben Mamoun C, Fuller L, Molestina RE, Florin-Christensen M, Schnittger L, Alzan HF, Suarez CE. Babesia microti Immunoreactive Rhoptry-Associated Protein-1 Paralogs Are Ancestral Members of the Piroplasmid-Confined RAP-1 Family. Pathogens 2021 Oct 26;10(11).
                doi: 10.3390/pathogens10111384pubmed: 34832541google scholar: lookup
              8. Idoko IS, Edeh RE, Adamu AM, Machunga-Mambula S, Okubanjo OO, Balogun EO, Adamu S, Johnson W, Kappmeyer L, Mousel M, Ueti MW. Molecular and Serological Detection of Piroplasms in Horses from Nigeria. Pathogens 2021 Apr 23;10(5).
                doi: 10.3390/pathogens10050508pubmed: 33922468google scholar: lookup
              9. Tirosh-Levy S, Mazuz ML, Savitsky I, Pinkas D, Gottlieb Y, Steinman A. Serological and Molecular Prevalence of Babesia caballi in Apparently Healthy Horses in Israel. Pathogens 2021 Apr 8;10(4).
                doi: 10.3390/pathogens10040445pubmed: 33917822google scholar: lookup
              10. Bastos RG, Sears KP, Dinkel KD, Kappmeyer L, Ueti MW, Knowles DP, Fry LM. Development of an Indirect ELISA to Detect Equine Antibodies to Theileria haneyi. Pathogens 2021 Feb 27;10(3).
                doi: 10.3390/pathogens10030270pubmed: 33673478google scholar: lookup
              11. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
                doi: 10.3390/pathogens9110926pubmed: 33171698google scholar: lookup
              12. Mahmoud MS, Kandil OM, Abu El-Ezz NT, Hendawy SHM, Elsawy BSM, Knowles DP, Bastos RG, Kappmeyer LS, Laughery JM, Alzan HF, Suarez CE. Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP4). Parasit Vectors 2020 Jul 22;13(1):369.
                doi: 10.1186/s13071-020-04241-9pubmed: 32698835google scholar: lookup
              13. Sunday Idoko I, Tirosh-Levy S, Leszkowicz Mazuz M, Mohammed Adam B, Sikiti Garba B, Wesley Nafarnda D, Steinman A. Genetic Characterization of Piroplasms in Donkeys and Horses from Nigeria. Animals (Basel) 2020 Feb 18;10(2).
                doi: 10.3390/ani10020324pubmed: 32085574google scholar: lookup
              14. Elata A, Mossaad E, Satti R, Matar N, Ohari Y, Xuan X, Inoue N, Suganuma K. Serological and molecular detection of selected hemoprotozoan parasites in donkeys in West Omdurman, Khartoum State, Sudan. J Vet Med Sci 2020 Mar 5;82(3):286-293.
                doi: 10.1292/jvms.19-0534pubmed: 31969541google scholar: lookup
              15. Aziz KJ, Al-Barwary LTO. Epidemiological Study of Equine Piroplasmosis (Theileria equi and Babesia caballi) by Microscopic Examination and Competitive-ELISA in Erbil Province North-Iraq. Iran J Parasitol 2019 Jul-Sep;14(3):404-412.
                pubmed: 31673258
              16. Onyiche TE, Suganuma K, Igarashi I, Yokoyama N, Xuan X, Thekisoe O. A Review on Equine Piroplasmosis: Epidemiology, Vector Ecology, Risk Factors, Host Immunity, Diagnosis and Control. Int J Environ Res Public Health 2019 May 16;16(10).
                doi: 10.3390/ijerph16101736pubmed: 31100920google scholar: lookup
              17. Lobanov VA, Peckle M, Massard CL, Brad Scandrett W, Gajadhar AA. Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis. Parasit Vectors 2018 Mar 2;11(1):125.
                doi: 10.1186/s13071-018-2751-6pubmed: 29499748google scholar: lookup
              18. Mahmoud MS, El-Ezz NT, Abdel-Shafy S, Nassar SA, El Namaky AH, Khalil WK, Knowles D, Kappmeyer L, Silva MG, Suarez CE. Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches. Parasit Vectors 2016 May 4;9:260.
                doi: 10.1186/s13071-016-1539-9pubmed: 27146413google scholar: lookup
              19. Awinda PO, Mealey RH, Williams LB, Conrad PA, Packham AE, Reif KE, Grause JF, Pelzel-McCluskey AM, Chung C, Bastos RG, Kappmeyer LS, Howe DK, Ness SL, Knowles DP, Ueti MW. Serum antibodies from a subset of horses positive for Babesia caballi by competitive enzyme-linked immunosorbent assay demonstrate a protein recognition pattern that is not consistent with infection. Clin Vaccine Immunol 2013 Nov;20(11):1752-7.
                doi: 10.1128/CVI.00479-13pubmed: 24049108google scholar: lookup
              20. Mosqueda J, Olvera-Ramirez A, Aguilar-Tipacamu G, Canto GJ. Current advances in detection and treatment of babesiosis. Curr Med Chem 2012;19(10):1504-18.
                doi: 10.2174/092986712799828355pubmed: 22360483google scholar: lookup
              21. Sun Y, Moreau E, Chauvin A, Malandrin L. The invasion process of bovine erythrocyte by Babesia divergens: knowledge from an in vitro assay. Vet Res 2011 May 11;42(1):62.
                doi: 10.1186/1297-9716-42-62pubmed: 21569363google scholar: lookup
              22. Schwint ON, Ueti MW, Palmer GH, Kappmeyer LS, Hines MT, Cordes RT, Knowles DP, Scoles GA. Imidocarb dipropionate clears persistent Babesia caballi infection with elimination of transmission potential. Antimicrob Agents Chemother 2009 Oct;53(10):4327-32.
                doi: 10.1128/AAC.00404-09pubmed: 19620328google scholar: lookup
              23. Goff WL, Johnson WC, Molloy JB, Jorgensen WK, Waldron SJ, Figueroa JV, Matthee O, Adams DS, McGuire TC, Pino I, Mosqueda J, Palmer GH, Suarez CE, Knowles DP, McElwain TF. Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle. Clin Vaccine Immunol 2008 Sep;15(9):1316-21.
                doi: 10.1128/CVI.00150-08pubmed: 18632921google scholar: lookup
              24. Goff WL, Molloy JB, Johnson WC, Suarez CE, Pino I, Rhalem A, Sahibi H, Ceci L, Carelli G, Adams DS, McGuire TC, Knowles DP, McElwain TF. Validation of a competitive enzyme-linked immunosorbent assay for detection of antibodies against Babesia bovis. Clin Vaccine Immunol 2006 Nov;13(11):1212-6.
                doi: 10.1128/CVI.00196-06pubmed: 16957062google scholar: lookup
              25. Boonchit S, Xuan X, Yokoyama N, Goff WL, Waghela SD, Wagner G, Igarashi I. Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies. J Clin Microbiol 2004 Apr;42(4):1601-4.
                doi: 10.1128/JCM.42.4.1601-1604.2004pubmed: 15071011google scholar: lookup
              26. Tamaki Y, Hirata H, Takabatake N, Bork S, Yokoyama N, Xuan X, Fujisaki K, Igarashi I. Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 2004 Jan;11(1):211-5.
                doi: 10.1128/cdli.11.1.211-215.2004pubmed: 14715570google scholar: lookup
              27. Goff WL, McElwain TF, Suarez CE, Johnson WC, Brown WC, Norimine J, Knowles DP. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein 1 epitope specifically identifies Babesia bovis-infected cattle. Clin Diagn Lab Immunol 2003 Jan;10(1):38-43.
                doi: 10.1128/cdli.10.1.38-43.2003pubmed: 12522037google scholar: lookup
              28. Boonchit S, Xuan X, Yokoyama N, Goff WL, Wagner G, Igarashi I. Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein 1 antigen against Babesia bovis for the detection of specific antibodies in cattle. J Clin Microbiol 2002 Oct;40(10):3771-5.
                doi: 10.1128/JCM.40.10.3771-3775.2002pubmed: 12354879google scholar: lookup
              29. Ozyörük F, Cheevers WP, Hullinger GA, McGuire TC, Hutton M, Knowles DP. Monoclonal antibodies to conformational epitopes of the surface glycoprotein of caprine arthritis-encephalitis virus: potential application to competitive-inhibition enzyme-linked immunosorbent assay for detecting antibodies in goat sera. Clin Diagn Lab Immunol 2001 Jan;8(1):44-51.
                doi: 10.1128/CDLI.8.1.44-51.2001pubmed: 11139194google scholar: lookup
              30. Cardillo NN, Villarino NF, Kappmeyer LS, Chung CJ, Suarez CE, Bastos RG. Tafenoquine succinate inhibits the growth of the equine piroplasmosis hemoparasites Theileria equi and Babesia caballi. Parasit Vectors 2026 Jan 27;19(1):61.
                doi: 10.1186/s13071-026-07262-ypubmed: 41593688google scholar: lookup
              31. Gupta KK, Gupta N, Kumar S, Srivastava M, Kumar P. Equine piroplasmosis: an emerging tick-borne threat to equine health. Trop Anim Health Prod 2026 Jan 5;58(1):29.
                doi: 10.1007/s11250-025-04829-2pubmed: 41489672google scholar: lookup
              32. Jia Z, Zhang Y, Zhao D, Wang H, Yu M, Liu Z, Zhang X, Cui J, Wang X. Research progress on diagnostic techniques for different Babesia species in persistent infections. Front Cell Infect Microbiol 2025;15:1575227.
                doi: 10.3389/fcimb.2025.1575227pubmed: 40453708google scholar: lookup
              33. Malgwi SA, Adeleke VT, Adeleke MA, Okpeku M. Multi-epitope Based Peptide Vaccine Candidate Against Babesia Infection From Rhoptry-Associated Protein 1 (RAP-1) Antigen Using Immuno-Informatics: An In Silico Approach. Bioinform Biol Insights 2024;18:11779322241287114.
                doi: 10.1177/11779322241287114pubmed: 39691583google scholar: lookup
              34. Mendoza FJ, Pérez-Écija A, Kappmeyer LS, Suarez CE, Bastos RG. New insights in the diagnosis and treatment of equine piroplasmosis: pitfalls, idiosyncrasies, and myths. Front Vet Sci 2024;11:1459989.
                doi: 10.3389/fvets.2024.1459989pubmed: 39205808google scholar: lookup
              35. Jongejan F, Du C, Papadopoulos E, Blanda V, Di Bella S, Cannella V, Guercio A, Vicari D, Tirosh-Levy S, Steinman A, Baneth G, van Keulen S, Hulsebos I, Berger L, Wang X. Diagnostic performance of a rapid immunochromatographic test for the simultaneous detection of antibodies to Theileria equi and Babesia caballi in horses and donkeys. Parasit Vectors 2024 Mar 28;17(1):160.
                doi: 10.1186/s13071-024-06253-1pubmed: 38549117google scholar: lookup
              36. Ahedor B, Otgonsuren D, Zhyldyz A, Guswanto A, Ngigi NMM, Valinotti MFR, Kothalawala H, Kalaichelvan N, Silva SSP, Kothalawala H, Acosta TJ, Sivakumar T, Yokoyama N. Development and evaluation of specific polymerase chain reaction assays for detecting Theileria equi genotypes. Parasit Vectors 2023 Nov 25;16(1):435.
                doi: 10.1186/s13071-023-06045-zpubmed: 38007442google scholar: lookup
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