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Home / Equine Research Bank / Vet Dermatol / Detection of papillomavirus-DNA in mesenchymal tumour cells ...
Veterinary dermatology2003; 14(1); 47-56; doi: 10.1046/j.1365-3164.2003.00324.x

Detection of papillomavirus-DNA in mesenchymal tumour cells and not in the hyperplastic epithelium of feline sarcoids.

Abstract: We examined 12 formalin-fixed paraffin-embedded feline skin tumours which had the histopathological features of fibropapillomas for the presence of papillomavirus (PV) DNA using touchdown polymerase chain reaction (PCR), DNA sequencing and nonradioactive in situ hybridization. Nine of the tumours contained a 102-bp PCR product demonstrated using consensus PV primers that amplify a portion of the L1 gene. The nucleotide sequences are closely related, but not identical to that of ovine PV type 2, rabbit oral PV and reindeer PV. The deduced amino acid sequences had strong homologies with the major capsid protein L1 of deer PV, bovine papillomavirus (BPV) 1 and BPV 2, and European elk PV. Although PV antigens were not detected in any of the tumours by immunohistochemistry, PV DNA was demonstrated in individual mesenchymal cells or cell nests of 4/12 tumours by in situ hybridization. A nonproductive infection of mesenchymal fibroblast-like tumour cells with a papillomavirus would explain the lack of PV antigen expression and the absence of PV DNA in the hyperplastic epithelium. Because these tumours and their pathogenesis are similar to equine sarcoids, we suggest that they should be reclassified as 'feline sarcoids' instead of fibropapillomas. Résumé Nous avons examiné 12 blocs parrafinés de tumeurs cutanées du chat ressemblant à des fibropapillomes afin de rechercher par PCR, séquençage de l’ADN et hybridisation non radioactive in situ la presence de papillomavirus (PV). Neuf tumeurs présentaient un produit de PCR 102 bp, mis en evidence par un primère PV qui amplifie une portion du gène L1. Les séquences nucléotidiques étaient proches, mais non identiques au PV ovin de type 2, au PV oral du lapin, et au PV du renne. Les séquences d’acides aminés avaient des homologies marquées avec la protéine de la capside L1 du cerf, du papillomavirus bovin de type 1 et 2. Alors que les techniques immunohistochimiques n’ont pas permis de mettre en évidence d’antigènes de PV, de l’ADN de PV a été retrouvé dans les cellules mésenchymateuses ou les cordons cellulaires de 4/12 tumeurs par hybridisation in situ. Une infection non productive des cellules tumorales mésenchymateuses fibroblastiques par un papillomavirus pourrait expliquer l’absence d’expression d’antigènes de PV et l’absence d’ADN de PV dans l’épithélium hyperplasique. Comme ces tumeurs sont semblables aux sarcoïdes équins, nous suggérons qu’elles soient reclassées comme des ‘sarcoïdes félins’ plutôt que comme des fibropapillomes. Resumen Examinamos 12 tumores cutáneos felinos, fijados en formalina e incluidos en parafina, con características histopatológicas de fibropapiloma, para determinar la presencia de DNA de papilomavirus (PV) utilizando una ‘touchdown’ PCR, secuenciación del DNA e hibridación in situ no radioactiva. Nueve de los tumores contenían un producto de la PCR de 102 pb, obtenido con cebadores consensuales (‘consensos primers’) de PV que amplifican una porción del gen L1. Las secuencias de nucleótidos están íntimamente relacionadas, pero no son idénticas al PV ovino tipo 2, PV oral del conejo o al PV del caribú. Las secuencias de aminoácidos deducidas tenían fuertes homologías con la proteína de la capside mayor L1 del PV del ciervo, PV bovino (PVB) 1 y el PVB 2, y PV del ciervo europeo. Mientras que los antígenos del PV no fueron detectados immunoquímicamente en ninguno de los tumores, ADN PV fue demostrado, mediante hibridación in situ, en células mesenquimatosas individuales o en grupos de células en 4/12 tumores. Una infección no productiva con un papilomavirus de las células fibroblásticas mesenquimatosas explicaría la falta de expresión de antígenos PV y la ausencia de DNA PV en el epitelio hiperplásico. Debido a que estos tumores y su patogénesis son similares a los sarcoides equinos, sugerimos que deberían clasificarse como ‘sarcoides felinos’ en lugar de fibropapilomas. Zusammenfassung Wir untersuchten 12 Formalin-fixierte, in Paraffin eingebettete Hauttumoren der Katze, die histopathologische Charakteristika von Fibropapillomen aufwiesen, auf die Präsenz von Papillomavirus (PV) DNS mittels PCR, DNS-Sequenzierung und nicht-radioaktiver in situ Hybridisierung. Neun der Tumoren enthielten ein mittels consensus PV Starter nachgewiesenes 102 bp PCR Produkt, dass einen Teil des L1 Gens amplifiziert. Die Nukleotidsequenzen sind mit denen von ovinem PV Typ 2, oralem Kaninchen PV, und Rentier PV verwandt. Die deduzierten Aminosäurensequenzen zeigten deutliche Homologie mit dem “major capsid protein L1” vom PV des Rehs, vom bovinen Papillomavirus (BPV) 1 und BPV 2 und vom PV des europäischen Elchs. Während mittels Immunohistochemie in keinem der Tumoren PV Antigene entdeckt wurden, wurde PV DNS in individuellen Mesenchymalzellen oder Zellnestern in 4 der 12 Tumoren mittels in situ Hybridisierung nachgewiesen. Eine nicht produktive Infektion von mesenchymalen Fibroblasten-ähnlichen Tumoren mit Papillomavirus könnte das Fehlen der PV Antigen-Exprimierung und die Abwesenheit der PV DNS im hyperplastischen Epithel erklären. Da diese Tumoren und ihre Pathogenese dem equinen Sarkoid ähnlich sind, schlagen wir vor, dass sie an Stelle von Fibropapillomen als feline Sarkoide reklassifiziert werden.
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Publication Date: 2003-02-27 PubMed ID: 12603685DOI: 10.1046/j.1365-3164.2003.00324.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied 12 feline skin tumours considered to have traits of fibropapillomas, exploring the presence of papillomavirus (PV) DNA. The presence of PV DNA was found in some tumour cells; however, PV antigens were not found within the tumours. The research concludes by suggesting the skin tumours should be reclassified as ‘feline sarcoids’, due to similarities with equine sarcoids.

Research Method

  • The researchers analyzed 12 feline skin tumours that had been fixed in formalin and embedded in paraffin and were histopathologically characterised as fibropapillomas.
  • The team sought the presence of papillomavirus (PV) DNA using a variety of methods, including touchdown polymerase chain reaction (PCR), DNA sequencing, and nonradioactive in situ hybridization.

Results

  • Nine of the studied tumours contained a 102-bp PCR product, which indicates the presence of a portion of the L1 gene from PV.
  • The nucleotide sequences from the tumours were observed to be similar but not identical to specific types of PV found in sheep, rabbits, and reindeer.
  • The amino acid sequences exhibited strong homologies with the major capsid protein L1 of deer PV, bovine papillomavirus (BPV) 1 and BPV 2, and European elk PV.
  • Despite these findings, no PV antigens were detected in any of the tumours using immunohistochemistry.
  • The PV DNA was found to be present in individual mesenchymal cells or cell nests in four out of twelve tumours when examined via in situ hybridization.

Conclusion and Implications

  • The lack of PV antigen expression and the absence of PV DNA in the hyperplastic epithelium were attributed to a nonproductive infection of mesenchymal fibroblast-like tumour cells by a PV, as per the researchers’ explanation.
  • Given the similarity of these tumours and their pathogenesis to equine sarcoids, the researchers suggested that they should be reclassified as ‘feline sarcoids’ rather than fibropapillomas. This categorisation could help improve our understanding of these types of tumours in cats and inform future research and treatment methods.

Cite This Article

APA
Teifke JP, Kidney BA, Löhr CV, Yager JA. (2003). Detection of papillomavirus-DNA in mesenchymal tumour cells and not in the hyperplastic epithelium of feline sarcoids. Vet Dermatol, 14(1), 47-56. https://doi.org/10.1046/j.1365-3164.2003.00324.x

Publication

Veterinary dermatology
ISSN: 0959-4493
NlmUniqueID: 9426187
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 47-56

Researcher Affiliations

Teifke, Jens P
  • Bundesforschungsanstalt für Viruskrankheiten der Tiere, Friedrich-Loeffler-Institut, Boddenblick 5a, D-17493 Greifswald, Insel Riems, Germany.
Kidney, Beverly A
  • Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Löhr, Christiane V
  • Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, USA.
Yager, Julie A
  • Department of Pathobiology, Ontario Veterinary College, University of Guelph, Ontario, Canada.

MeSH Terms

  • Animals
  • Antigens, Viral / analysis
  • Base Sequence
  • Cat Diseases / pathology
  • Cat Diseases / virology
  • Cats
  • DNA Primers
  • DNA, Viral / analysis
  • Female
  • Fibroma / veterinary
  • Fibroma / virology
  • Immunohistochemistry
  • In Situ Hybridization / veterinary
  • Male
  • Molecular Sequence Data
  • Papillomaviridae / genetics
  • Papillomaviridae / immunology
  • Papillomaviridae / isolation & purification
  • Polymerase Chain Reaction / veterinary
  • Skin Neoplasms / veterinary
  • Skin Neoplasms / virology

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