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Home / Equine Research Bank / Vet Res Commun / Detection of reaginic antibodies against Faenia rectivirgula...
Veterinary research communications2010; 34(8); 719-726; doi: 10.1007/s11259-010-9440-z

Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.

Abstract: Reaginic antibodies, mainly of the IgE and some IgG subclasses, play an important role in the induction of type I immediate hypersensitivity reactions. These antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. Previously, several studies have investigated the role of reaginic antibodies in the pathogenesis of RAO. However, whereas immunological aspects of RAO have been extensively studied, the precise sequence of events is still not well understood and role of IgE in this disease still remains controversial. Therefore, in this study a bioassay was developed for reaginic antibody determination in serum from RAO-affected horses in order to determine the etiology of disease. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from RAO-affected or unaffected horses and one of the RAO antigens (Faenia rectivirgula). Results demonstrated that 15% of samples from the RAO-affected horses reacted positively in this in vitro bioassay, whereas the samples from unaffected horses did not. This bioassay indicates that reaginic antibodies could be involved in the immunological mechanism leading to RAO; and this technique may facilitate future research in other allergic diseases in horses.
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Publication Date: 2010-09-03 PubMed ID: 20814741DOI: 10.1007/s11259-010-9440-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the role of certain antibodies in horses suffering from Recurrent Airway Obstruction (RAO) using a new assay or test. The test was conducted using serum or blood samples from horses both affected and unaffected by RAO to better understand the disease’s nature and possibly contribute to research on other allergies in horses.

Background of the Study

  • Reaginic antibodies, primarily IgE and some subclasses of IgG, are known to contribute to type I immediate hypersensitivity reactions. These are rapid allergic responses that typically occur within minutes of exposure to an allergen.
  • These antibodies attach to high affinity receptors found on mast cells and basophils’ cellular membranes. This relation makes them central to the study.
  • Reaginic antibodies’ role in the development of RAO in horses has been explored in other studies, but the exact course of events and the part played by IgE particularly is still unclear.

Objective and Methodology of the Study

  • This study aimed to develop a bioassay to detect reaginic antibodies in the serum of horses suffering from RAO, to determine the disease’s cause.
  • For this, the assay measured calcium mobilization in RBL-2H3 cells after incubating them with horse serum from both RAO ridden and healthy horses. One of the RAO antigens, Faenia rectivirgula, was also introduced during the course of the study.

Findings of the Study

  • The results showed that 15% of the samples from the RAO-affected horses reacted positively in the developed in vitro bioassay.
  • In contrast, the samples from the unaffected horses did not return any reaction.
  • These results indicate that reaginic antibodies might have a role in the immunological sequence leading to RAO.
  • The newly developed assay can be a useful tool in future research related to other allergic diseases in horses.

Cite This Article

APA
Morán G, Folch H, Araya O, Burgos R, Barria M. (2010). Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay. Vet Res Commun, 34(8), 719-726. https://doi.org/10.1007/s11259-010-9440-z

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 34
Issue: 8
Pages: 719-726

Researcher Affiliations

Morán, Gabriel
  • Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, Valdivia, Chile. gmoran@uach.cl
Folch, Hugo
    Araya, Oscar
      Burgos, Rafael
        Barria, Miguel

          MeSH Terms

          • Actinobacteria / immunology
          • Airway Obstruction / blood
          • Airway Obstruction / immunology
          • Airway Obstruction / veterinary
          • Animals
          • Antibodies, Bacterial / blood
          • Biological Assay / methods
          • Biological Assay / veterinary
          • Cell Line, Tumor
          • Horse Diseases / blood
          • Horse Diseases / diagnosis
          • Horse Diseases / immunology
          • Horses
          • Hypersensitivity / blood
          • Hypersensitivity / immunology
          • Hypersensitivity / veterinary
          • Reagins / analysis
          • Reagins / immunology

          References

          This article includes 36 references
          1. Tkaczyk C, Gilfillan AM. Fc(epsilon)Ri-dependent signaling pathways in human mast cells.. Clin Immunol 2001 May;99(2):198-210.
            pubmed: 11318592doi: 10.1006/clim.2001.4992google scholar: lookup
          2. Eder C, Curik I, Brem G, Crameri R, Bodo I, Habe F, Lazary S, Sölkner J, Marti E. Influence of environmental and genetic factors on allergen-specific immunoglobulin-E levels in sera from Lipizzan horses.. Equine Vet J 2001 Nov;33(7):714-20.
            pubmed: 11770995doi: 10.2746/042516401776249264google scholar: lookup
          3. Larsen HJ, Bakke SH, Mehl R. Intradermal challenge of Icelandic horses in Norway and Iceland with extracts of Culicoides spp.. Acta Vet Scand 1988;29(3-4):311-4.
            pubmed: 3256230doi: 10.1186/BF03548623google scholar: lookup
          4. Morán G, Burgos R, Araya O, Folch H. In vitro bioassay to detect reaginic antibodies from the serum of horses affected with recurrent airway obstruction.. Vet Res Commun 2010 Jan;34(1):91-9.
            pubmed: 20012188doi: 10.1007/s11259-009-9334-0google scholar: lookup
          5. Ungar-Waron H, Braverman Y, Gluckman A, Trainin Z. Immunogenicity and allergenicity of Culicoides imicola (Diptera: Ceratopogonidae) extracts.. Zentralbl Veterinarmed B 1990 Feb;37(1):64-72.
            pubmed: 2346072doi: 10.1111/j.1439-0450.1990.tb01027.xgoogle scholar: lookup
          6. McGorum BC, Dixon PM, Halliwell RE. Evaluation of intradermal mould antigen testing in the diagnosis of equine chronic obstructive pulmonary disease.. Equine Vet J 1993 Jul;25(4):273-5.
            pubmed: 8354210doi: 10.1111/j.2042-3306.1993.tb02962.xgoogle scholar: lookup
          7. Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties.. J Biol Chem 1985 Mar 25;260(6):3440-50.
            pubmed: 3838314
          8. Wagner B, Radbruch A, Rohwer J, Leibold W. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE.. Vet Immunol Immunopathol 2003 Mar 20;92(1-2):45-60.
            pubmed: 12628763doi: 10.1016/s0165-2427(03)00007-2google scholar: lookup
          9. Curik I, Fraser D, Eder C, Achmann R, Swinburne J, Crameri R, Brem G, Sölkner J, Marti E. Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse.. Genet Sel Evol 2003;35 Suppl 1(Suppl 1):S177-90.
            pubmed: 12927090doi: 10.1186/1297-9686-35-S1-S177google scholar: lookup
          10. Scharenberg AM, Kinet JP. Early events in mast cell signal transduction.. Chem Immunol 1995;61:72-87.
            pubmed: 7662147
          11. Zhang J, Siraganian RP. CD45 is essential for Fc epsilon RI signaling by ZAP70, but not Syk, in Syk-negative mast cells.. J Immunol 1999 Sep 1;163(5):2508-16.
            pubmed: 10452987
          12. Halliwell RE, McGorum BC, Irving P, Dixon PM. Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.. Vet Immunol Immunopathol 1993 Oct;38(3-4):201-15.
            pubmed: 8291200doi: 10.1016/0165-2427(93)90081-egoogle scholar: lookup
          13. Schmallenbach KH, Rahman I, Sasse HH, Dixon PM, Halliwell RE, McGorum BC, Crameri R, Miller HR. Studies on pulmonary and systemic Aspergillus fumigatus-specific IgE and IgG antibodies in horses affected with chronic obstructive pulmonary disease (COPD).. Vet Immunol Immunopathol 1998 Dec 11;66(3-4):245-56.
            pubmed: 9880102doi: 10.1016/s0165-2427(98)00202-5google scholar: lookup
          14. Ishizaka K, Ishizaka T. Physicochemical properties of reaginic antibody. 1. Association of reaginic activity with an immunoglobulin other than gammaA- or gammaG-globulin.. J Allergy 1966 Mar;37(3):169-85.
            pubmed: 4160257doi: 10.1016/0021-8707(66)90091-8google scholar: lookup
          15. Wagner B. IgE in horses: occurrence in health and disease.. Vet Immunol Immunopathol 2009 Nov 15;132(1):21-30.
            pubmed: 19819562doi: 10.1016/j.vetimm.2009.09.011google scholar: lookup
          16. McGorum BC, Dixon PM, Halliwell RE. Responses of horses affected with chronic obstructive pulmonary disease to inhalation challenges with mould antigens.. Equine Vet J 1993 Jul;25(4):261-7.
            pubmed: 8354208doi: 10.1111/j.2042-3306.1993.tb02960.xgoogle scholar: lookup
          17. McPherson EA, Lawson GH, Murphy JR, Nicholson JM, Breeze RG, Pirie HM. Chronic obstructive pulmonary disease (COPD) in horses: aetiological studies: responses to intradermal and inhalation antigenic challenge.. Equine Vet J 1979 Jul;11(3):159-66.
            pubmed: 385306doi: 10.1111/j.2042-3306.1979.tb01330.xgoogle scholar: lookup
          18. Robinson NE. International Workshop on Equine Chronic Airway Disease. Michigan State University 16-18 June 2000.. Equine Vet J 2001 Jan;33(1):5-19.
            pubmed: 11191611doi: 10.2746/042516401776767412google scholar: lookup
          19. Adamczewski M, Kinet JP. The high-affinity receptor for immunoglobulin E.. Chem Immunol 1994;59:173-90.
            pubmed: 7945926
          20. Robinson NE, Olszewski MA, Boehler D, Berney C, Hakala J, Matson C, Derksen FJ. Relationship between clinical signs and lung function in horses with recurrent airway obstruction (heaves) during a bronchodilator trial.. Equine Vet J 2000 Sep;32(5):393-400.
            pubmed: 11037260doi: 10.2746/042516400777591147google scholar: lookup
          21. Hellberg W, Wilson AD, Mellor P, Doherr MG, Torsteinsdottir S, Zurbriggen A, Jungi T, Marti E. Equine insect bite hypersensitivity: immunoblot analysis of IgE and IgG subclass responses to Culicoides nubeculosus salivary gland extract.. Vet Immunol Immunopathol 2006 Sep 15;113(1-2):99-112.
            pubmed: 16797724doi: 10.1016/j.vetimm.2006.04.009google scholar: lookup
          22. Derksen FJ, Robinson NE, Scott JS, Stick JA. Aerosolized Micropolyspora faeni antigen as a cause of pulmonary dysfunction in ponies with recurrent airway obstruction (heaves).. Am J Vet Res 1988 Jun;49(6):933-8.
            pubmed: 3400931
          23. Ali H, Maeyama K, Sagi-Eisenberg R, Beaven MA. Antigen and thapsigargin promote influx of Ca2+ in rat basophilic RBL-2H3 cells by ostensibly similar mechanisms that allow filling of inositol 1,4,5-trisphosphate-sensitive and mitochondrial Ca2+ stores.. Biochem J 1994 Dec 1;304 ( Pt 2)(Pt 2):431-40.
            pubmed: 7998977doi: 10.1042/bj3040431google scholar: lookup
          24. Repetto B, Bandara G, Kado-Fong H, Larigan JD, Wiggan GA, Pocius D, Basu M, Gilfillan AM, Kochan JP. Functional contributions of the FcepsilonRIalpha and FepsilonRIgamma subunit domains in FcepsilonRI-mediated signaling in mast cells.. J Immunol 1996 Jun 15;156(12):4876-83.
            pubmed: 8648137
          25. Derksen FJ, Robinson NE, Armstrong PJ, Stick JA, Slocombe RF. Airway reactivity in ponies with recurrent airway obstruction (heaves).. J Appl Physiol (1985) 1985 Feb;58(2):598-604.
            pubmed: 3980362doi: 10.1152/jappl.1985.58.2.598google scholar: lookup
          26. Metzger H. The receptor with high affinity for IgE.. Immunol Rev 1992 Feb;125:37-48.
            pubmed: 1532373doi: 10.1111/j.1600-065x.1992.tb00624.xgoogle scholar: lookup
          27. Künzle F, Gerber V, Van Der Haegen A, Wampfler B, Straub R, Marti E. IgE-bearing cells in bronchoalveolar lavage fluid and allergen-specific IgE levels in sera from RAO-affected horses.. J Vet Med A Physiol Pathol Clin Med 2007 Feb;54(1):40-7.
            pubmed: 17359454doi: 10.1111/j.1439-0442.2007.00870.xgoogle scholar: lookup
          28. Ching TT, Hsu AL, Johnson AJ, Chen CS. Phosphoinositide 3-kinase facilitates antigen-stimulated Ca(2+) influx in RBL-2H3 mast cells via a phosphatidylinositol 3,4,5-trisphosphate-sensitive Ca(2+) entry mechanism.. J Biol Chem 2001 May 4;276(18):14814-20.
            pubmed: 11278575doi: 10.1074/jbc.M009851200google scholar: lookup
          29. Prouvost-Danon A, Binaghi RA, Abadie A. Effect of heating at 56 degrees C on mouse IgE antibodies.. Immunochemistry 1977 Feb;14(2):81-4.
            pubmed: 15944doi: 10.1016/0019-2791(77)90284-1google scholar: lookup
          30. Wilson BS, Kapp N, Lee RJ, Pfeiffer JR, Martinez AM, Platt Y, Letourneur F, Oliver JM. Distinct functions of the Fc epsilon R1 gamma and beta subunits in the control of Fc epsilon R1-mediated tyrosine kinase activation and signaling responses in RBL-2H3 mast cells.. J Biol Chem 1995 Feb 24;270(8):4013-22.
            pubmed: 7876149doi: 10.1074/jbc.270.8.4013google scholar: lookup
          31. Marti E, Gerber V, Wilson AD, Lavoie JP, Horohov D, Crameri R, Lunn DP, Antczak D, Björnsdóttir S, Björnsdóttir TS, Cunningham F, Dérer M, Frey R, Hamza E, Horin P, Heimann M, Kolm-Stark G, Olafsdóttir G, Ramery E, Russell C, Schaffartzik A, Svansson V, Torsteinsdóttir S, Wagner B. Report of the 3rd Havemeyer workshop on allergic diseases of the Horse, Hólar, Iceland, June 2007.. Vet Immunol Immunopathol 2008 Dec 15;126(3-4):351-61.
            pubmed: 18775570doi: 10.1016/j.vetimm.2008.07.008google scholar: lookup
          32. Eder C, Crameri R, Mayer C, Eicher R, Straub R, Gerber H, Lazary S, Marti E. Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.. Vet Immunol Immunopathol 2000 Mar 15;73(3-4):241-53.
            pubmed: 10713338doi: 10.1016/s0165-2427(00)00154-9google scholar: lookup
          33. Kim TD, Eddlestone GT, Mahmoud SF, Kuchtey J, Fewtrell C. Correlating Ca2+ responses and secretion in individual RBL-2H3 mucosal mast cells.. J Biol Chem 1997 Dec 12;272(50):31225-9.
            pubmed: 9395446doi: 10.1074/jbc.272.50.31225google scholar: lookup
          34. Jackson KA, Stott JL, Horohov DW, Watson JL. IL-4 induced CD23 (FcepsilonRII) up-regulation in equine peripheral blood mononuclear cells and pulmonary alveolar macrophages.. Vet Immunol Immunopathol 2004 Oct;101(3-4):243-50.
            pubmed: 15350754doi: 10.1016/j.vetimm.2004.05.001google scholar: lookup
          35. Tahon L, Baselgia S, Gerber V, Doherr MG, Straub R, Robinson NE, Marti E. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009 Jan 15;127(1-2):85-93.
            pubmed: 19027178doi: 10.1016/j.vetimm.2008.09.021google scholar: lookup
          36. Gerber V, Lindberg A, Berney C, Robinson NE. Airway mucus in recurrent airway obstruction--short-term response to environmental challenge.. J Vet Intern Med 2004 Jan-Feb;18(1):92-7.
            pubmed: 14765737doi: 10.1892/0891-6640(2004)18<92:amirao>2.0.co;2google scholar: lookup

          Citations

          This article has been cited 6 times.
          1. Di Pietro R, Dubuc V, Manguin E, Giroux-Lafond R, Bédard C, Boivin R, Lavoie JP, Vesper SJ, Leclere M. Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma.. Am J Vet Res 2022 May 8;83(6).
            doi: 10.2460/ajvr.21.09.0143pubmed: 35524958google scholar: lookup
          2. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
            doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
          3. White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma.. Sci Rep 2019 Oct 23;9(1):15170.
            doi: 10.1038/s41598-019-51820-7pubmed: 31645629google scholar: lookup
          4. Sarmiento J, Perez B, Morales N, Henriquez C, Vidal L, Folch H, Galecio JS, Morán G. Apoptotic effects of tamoxifen on leukocytes from horse peripheral blood and bronchoalveolar lavage fluid.. Vet Res Commun 2013 Dec;37(4):333-8.
            doi: 10.1007/s11259-013-9571-0pubmed: 23846832google scholar: lookup
          5. Moran G, Folch H, Henriquez C, Ortloff A, Barria M. Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation.. Vet Res Commun 2012 Dec;36(4):251-8.
            doi: 10.1007/s11259-012-9534-xpubmed: 23011757google scholar: lookup
          6. Moran G, Buechner-Maxwell VA, Folch H, Henriquez C, Galecio JS, Perez B, Carrasco C, Barria M. Increased apoptosis of CD4 and CD8 T lymphocytes in the airways of horses with recurrent airway obstruction.. Vet Res Commun 2011 Oct;35(7):447-56.
            doi: 10.1007/s11259-011-9482-xpubmed: 21594641google scholar: lookup
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