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Veterinary research communications2010; 34(8); 719-726; doi: 10.1007/s11259-010-9440-z

Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay.

Abstract: Reaginic antibodies, mainly of the IgE and some IgG subclasses, play an important role in the induction of type I immediate hypersensitivity reactions. These antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. Previously, several studies have investigated the role of reaginic antibodies in the pathogenesis of RAO. However, whereas immunological aspects of RAO have been extensively studied, the precise sequence of events is still not well understood and role of IgE in this disease still remains controversial. Therefore, in this study a bioassay was developed for reaginic antibody determination in serum from RAO-affected horses in order to determine the etiology of disease. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from RAO-affected or unaffected horses and one of the RAO antigens (Faenia rectivirgula). Results demonstrated that 15% of samples from the RAO-affected horses reacted positively in this in vitro bioassay, whereas the samples from unaffected horses did not. This bioassay indicates that reaginic antibodies could be involved in the immunological mechanism leading to RAO; and this technique may facilitate future research in other allergic diseases in horses.
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Publication Date: 2010-09-03 PubMed ID: 20814741DOI: 10.1007/s11259-010-9440-zGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the role of certain antibodies in horses suffering from Recurrent Airway Obstruction (RAO) using a new assay or test. The test was conducted using serum or blood samples from horses both affected and unaffected by RAO to better understand the disease’s nature and possibly contribute to research on other allergies in horses.

Background of the Study

  • Reaginic antibodies, primarily IgE and some subclasses of IgG, are known to contribute to type I immediate hypersensitivity reactions. These are rapid allergic responses that typically occur within minutes of exposure to an allergen.
  • These antibodies attach to high affinity receptors found on mast cells and basophils’ cellular membranes. This relation makes them central to the study.
  • Reaginic antibodies’ role in the development of RAO in horses has been explored in other studies, but the exact course of events and the part played by IgE particularly is still unclear.

Objective and Methodology of the Study

  • This study aimed to develop a bioassay to detect reaginic antibodies in the serum of horses suffering from RAO, to determine the disease’s cause.
  • For this, the assay measured calcium mobilization in RBL-2H3 cells after incubating them with horse serum from both RAO ridden and healthy horses. One of the RAO antigens, Faenia rectivirgula, was also introduced during the course of the study.

Findings of the Study

  • The results showed that 15% of the samples from the RAO-affected horses reacted positively in the developed in vitro bioassay.
  • In contrast, the samples from the unaffected horses did not return any reaction.
  • These results indicate that reaginic antibodies might have a role in the immunological sequence leading to RAO.
  • The newly developed assay can be a useful tool in future research related to other allergic diseases in horses.

Cite This Article

APA
Morán G, Folch H, Araya O, Burgos R, Barria M. (2010). Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay. Vet Res Commun, 34(8), 719-726. https://doi.org/10.1007/s11259-010-9440-z

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 34
Issue: 8
Pages: 719-726

Researcher Affiliations

Morán, Gabriel
  • Department of Pharmacology, Faculty of Veterinary Science, Universidad Austral de Chile, Valdivia, Chile. gmoran@uach.cl
Folch, Hugo
    Araya, Oscar
      Burgos, Rafael
        Barria, Miguel

          MeSH Terms

          • Actinobacteria / immunology
          • Airway Obstruction / blood
          • Airway Obstruction / immunology
          • Airway Obstruction / veterinary
          • Animals
          • Antibodies, Bacterial / blood
          • Biological Assay / methods
          • Biological Assay / veterinary
          • Cell Line, Tumor
          • Horse Diseases / blood
          • Horse Diseases / diagnosis
          • Horse Diseases / immunology
          • Horses
          • Hypersensitivity / blood
          • Hypersensitivity / immunology
          • Hypersensitivity / veterinary
          • Reagins / analysis
          • Reagins / immunology

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          Citations

          This article has been cited 6 times.
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