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Home / Equine Research Bank / J Vet Sci / Detection of Streptococcus dysgalactiae subsp. equisimilis i...
Journal of veterinary science2010; 11(1); 67-72; doi: 10.4142/jvs.2010.11.1.67

Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR.

Abstract: Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. equisimilis is generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable epidemiological studies and effective investigation of outbreaks involving these bacteria. In this study, PCR protocols previously described in cattle and in humans to detect the species S. dysgalactiae and the subspecies equisimilis were evaluated to detect specific sequences in equine samples. For this purpose, 99 monolateral nasal swabs were collected from horses from stud farms with a history of S. equisimilis infection and were tested blindly by bacteriological isolation and by single and duplex PCR. DNA for PCR was extracted both from the colonies grown on agar media and from enrichment broth aliquots after incubation with nasal swab samples. S. equisimilis was identified by bacteriological isolation in 23 out of 99 swab samples, and PCR assays on these colonies were fully concordant with bacteriological identification (kappa statistic = 1.00). In addition, PCR of the enrichment broth aliquots confirmed the bacteriological results and detected S. equisimilis in 6 samples more than the bacteriological examination (kappa statistic = 0.84). The PCR protocols appeared to be reliable for the rapid identification of S. equisimilis in equine nasal swab samples, and could be useful for microbiological diagnosis.
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Publication Date: 2010-03-03 PubMed ID: 20195067PubMed Central: PMC2833432DOI: 10.4142/jvs.2010.11.1.67Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a study conducted to evaluate the effectiveness of Polymerase Chain Reaction (PCR) in detecting Streptococcus dysgalactiae subsp. equisimilis, a bacteria causing severe diseases, in horses. The study found that the PCR protocols are a reliable method for the rapid identification of this bacterium in equine nasal swab samples.

Objective of the Research

  • The primary aim of the research was to assess the effectiveness of PCR protocols, previously used in cattle and humans, for detecting S. dysgalactiae and its subspecies equisimilis in horses.
  • It aimed to aid in the rapid and sensitive diagnosis of these bacteria, which cause severe diseases in humans and can infect other animal species.

Research Methodology

  • The researchers collected 99 nasal swabs from horses residing in stud farms with a history of S. equisimilis infection.
  • These samples were then tested blindly through bacteriological isolation and by single and duplex PCR.
  • The DNA for PCR was extracted from the colonies grown on agar media and from enrichment broth aliquots after incubation with nasal swab samples.

Research Findings

  • The bacteriological isolation identified S. equisimilis in 23 out of 99 swab samples.
  • PCR assays performed on these colonies were found to be fully concordant with bacteriological identification (kappa statistic = 1.00).
  • Apart from confirming the bacteriological results, the PCR of the enrichment broth aliquots detected S. equisimilis in an additional 6 samples (kappa statistic = 0.84).

Conclusion of the Research

  • The researchers concluded that PCR protocols are a reliable and effective tool for the rapid identification of S. equisimilis in equine nasal swab samples.
  • This method could be helpful in conducting epidemiological studies and investigating outbreaks of diseases caused by these bacteria.
  • Overall, the use of PCR protocols in microbiological diagnosis was recommended by the researchers.

Cite This Article

APA
Preziuso S, Laus F, Tejeda AR, Valente C, Cuteri V. (2010). Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR. J Vet Sci, 11(1), 67-72. https://doi.org/10.4142/jvs.2010.11.1.67

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 11
Issue: 1
Pages: 67-72

Researcher Affiliations

Preziuso, Silvia
  • Department of Veterinary Science, University of Camerino, Via Circonvallazione 93/95, Matelica, Italy. silvia.preziuso@unicam.it
Laus, Fulvio
    Tejeda, Aurora Romero
      Valente, Carlo
        Cuteri, Vincenzo

          MeSH Terms

          • Animals
          • DNA, Bacterial / chemistry
          • DNA, Bacterial / genetics
          • Horse Diseases / diagnosis
          • Horse Diseases / microbiology
          • Horses
          • Limit of Detection
          • Male
          • Nasopharynx / microbiology
          • Polymerase Chain Reaction / methods
          • Polymerase Chain Reaction / veterinary
          • Respiratory Tract Infections / diagnosis
          • Respiratory Tract Infections / microbiology
          • Respiratory Tract Infections / veterinary
          • Sensitivity and Specificity
          • Streptococcal Infections / diagnosis
          • Streptococcal Infections / microbiology
          • Streptococcal Infections / veterinary
          • Streptococcus / genetics
          • Streptococcus / isolation & purification

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          Citations

          This article has been cited 6 times.
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