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Drug testing and analysis2022; 15(3); 361-367; doi: 10.1002/dta.3406

Detection of the growth hormone secretagogue MK-0677 in equine hair following oral administration.

Abstract: MK-0677 (ibutamoren) is an orally active non-peptide growth hormone secretagogue that binds to the ghrelin receptor stimulating the secretion of endogenous growth hormone. It is one of the most prevalent performance-enhancing compounds currently available online and is potentially subject to abuse both in human and equine sports. The aim of the current study was to investigate whether it could be detected in equine hair following oral administration of MK-0677 mesylate to a Thoroughbred racehorse. MK-0677 and its O-dealkylated metabolite were extracted using an existing method for prohibited substances in equine hair and analysed by liquid chromatography tandem mass spectrometry. This enabled the detection of MK-0677 in all hair samples collected, up to 209 days in mane and 358 days in tail. A follow-up methodology with an extensive wash procedure was carried out for selected hair samples, which unambiguously verified the presence of MK-0677. Wash criteria to differentiate between internal incorporation (via bloodstream) and external deposition (via sweat and sebum) was also assessed and indicated internal incorporation for the samples collected at later time points (≥52 days) and a combination of internal incorporation and external deposition for hair samples collected at the earlier time point (2 days).
© 2022 John Wiley & Sons, Ltd.
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Publication Date: 2022-12-19 PubMed ID: 36354265DOI: 10.1002/dta.3406Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the detection of a growth hormone known as MK-0677 (ibutamoren) in horse hair. It studies the evidence of this hormone in the mane and tail of a Thoroughbred racehorse up to a period of about a year after oral administration.

Introduction and Research Objective

  • The article introduces MK-0677 as a high-performance synthetic hormone that is available online. It is potentially abused in horse and human sports since it triggers the secretion of natural growth hormones. The objective of the study is to detect the presence and assess the lifespan of MK-0677 in horse hair following oral administration.

Methodology

  • The researchers administered MK-0677 mesylate orally to a Thoroughbred racehorse.
  • They then extracted the hormone and its O-dealkylated metabolite from the horse hair.
  • They performed the extraction using an existing method for prohibited substances in horse hair.
  • The extracted samples were analysed by liquid chromatography tandem mass spectrometry.

Results

  • After the analysis, the presence of MK-0677 was detected in all the collected horse hair samples.
  • The hormone was found to last up to 209 days in the mane and 358 days in the tail samples.
  • A follow-up method with a comprehensive washing procedure confirmed the unambiguous presence of MK-0677 in the selected hair samples.

Assessment of Incorporation

  • The researchers also evaluated criteria to distinguish between internal incorporation (when the hormone entered hair through the bloodstream) and external deposition (when the hormone is present in the hair due to sweat and sebum).
  • The results indicated internal incorporation for samples collected later (at least 52 days after administration).
  • For the earlier hair samples, collected 2 days after administration, it was a mix of both internal incorporation and external deposition.

Cite This Article

APA
Viljanto M, Cutler C, Taylor P, Habershon-Butcher J, Gray B. (2022). Detection of the growth hormone secretagogue MK-0677 in equine hair following oral administration. Drug Test Anal, 15(3), 361-367. https://doi.org/10.1002/dta.3406

Publication

Drug testing and analysis
ISSN: 1942-7611
NlmUniqueID: 101483449
Country: England
Language: English
Volume: 15
Issue: 3
Pages: 361-367

Researcher Affiliations

Viljanto, Marjaana
  • LGC, Fordham, Cambridgeshire, UK.
Cutler, Charlotte
  • LGC, Fordham, Cambridgeshire, UK.
Taylor, Polly
  • LGC, Fordham, Cambridgeshire, UK.
Habershon-Butcher, Jocelyn
  • British Horseracing Authority, London, UK.
Gray, Bob
  • LGC, Fordham, Cambridgeshire, UK.

MeSH Terms

  • Humans
  • Animals
  • Horses
  • Secretagogues / analysis
  • Hair / chemistry
  • Growth Hormone
  • Administration, Oral

References

This article includes 23 references
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