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Home / Equine Research Bank / Infect Immun / Detection of tumor-specific antigens in an equine sarcoid ce...
Infection and immunity1974; 9(4); 714-718; doi: 10.1128/iai.9.4.714-718.1974

Detection of tumor-specific antigens in an equine sarcoid cell line.

Abstract: Indirect immunofluorescence and lymphocyte cytotoxicity experiments demonstrated the presence of a tumor-specific antigen(s) on the surface of cells from an equine sarcoid cell line (Mc1). Autologous serum (taken from the horse from which the Mc1 cells were derived) and sera from three other sarcoid-bearing horses revealed a similar membrane immunofluorescence when reacted with Mc1 cells, indicating the existence of cross-reacting antibodies. Results of serum colony inhibition experiments indicate that these antibodies are not cytotoxic. Incubation of Mc1 cells with autologous lymphocytes resulted in a toxicity to Mc1 cells, thereby demonstrating a cell-mediated immune reaction to autologous tumor cells.
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Publication Date: 1974-04-01 PubMed ID: 4595759PubMed Central: PMC414871DOI: 10.1128/iai.9.4.714-718.1974Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored the presence of tumor-specific antigens on the surface of cells from an equine sarcoid cell line and found they could instigate an immune response.

Study Focus and Methodology

  • The main focus of this research was the detection of tumor-special antigens on the surface of cells from an equine sarcoid cell line. Antigens are substances capable of inducing specific immune responses, which can be beneficial in cancer therapy because they target only cancerous cells. Thus, these identified antigens could potentially be significant in treating cancers in horses.
  • The researchers carried out indirect immunofluorescence and lymphocyte cytotoxicity experiments to determine the presence of these antigens. Indirect immunofluorescence is a two-step process where a sample is first incubated with primary antibodies, then with secondary fluorescent antibodies that bind to the primary antibodies to allow visual detection of the antigen. Lymphocyte cytotoxicity tests evaluate the ability of activated lymphocytes to kill targeted cells.

Findings of the Study

  • The researchers found that there was indeed a tumor-specific antigen present on the surface of cells from an equine sarcoid cell line, called Mc1. This observation was confirmed by the reaction of the serum taken from the same horse (autologous serum) and serum from three other sarcoid-bearing horses when exposed to the Mc1 cells.
  • These reactions suggested the presence of cross-reacting antibodies, or antibodies in the horse’s serum that are capable of binding to multiple related antigens simultaneously. The detection of such cross-reacting antibodies exposes further the immune system’s recognition of these antigens.
  • The study also looked into the cytotoxicity of these antibodies. The serum colony inhibition experiments showed that these cross-reacting antibodies are not cytotoxic, meaning they do not kill cells directly.
  • Lastly, an interesting result that was obtained from the study was that exposing Mc1 cells to autologous lymphocytes resulted in a cell-mediated immune response wherein the immune cells demonstrated toxicity to the autologous tumor cells. This means that the immune cells of the horse recognized the tumor-specific antigens and responded accordingly by killing the tumor cells, demonstrating the potential anti-cancer properties of these antigens.

Cite This Article

APA
Watson RE, Larson KA. (1974). Detection of tumor-specific antigens in an equine sarcoid cell line. Infect Immun, 9(4), 714-718. https://doi.org/10.1128/iai.9.4.714-718.1974

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 9
Issue: 4
Pages: 714-718

Researcher Affiliations

Watson, R E
    Larson, K A

      MeSH Terms

      • Animals
      • Antigens, Neoplasm / analysis
      • Cell Division
      • Cell Line
      • Culture Techniques
      • Cytotoxicity Tests, Immunologic
      • Fluorescent Antibody Technique
      • Horses
      • Immune Sera
      • Lymphocytes / immunology
      • Rabbits / immunology
      • Sarcoidosis / immunology

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      Citations

      This article has been cited 3 times.
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