Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry.
Abstract: A rapid method for the quantification of amiodarone and desethylamiodarone in animal plasma using high-performance liquid chromatography combined with UV detection (HPLC-UV) is presented. The sample preparation includes a simple deproteinisation step with acetonitrile. In addition, a sensitive method for the quantification of amiodarone and desethylamiodarone in horse plasma and urine using high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) is described. The sample preparation includes a solid-phase extraction (SPE) with a SCX column. Tamoxifen is used as an internal standard for both chromatographic methods. Chromatographic separation is achieved on an ODS Hypersil column using isocratic elution with 0.01% diethylamine and acetonitrile as mobile phase for the HPLC-UV method and with 0.1% formic acid and acetonitrile as mobile phase for the LC-MS/MS method. For the HPLC-UV method, good linearity was observed in the range 0-5 microg ml(-1), and in the range 0-1 microg ml(-1) for the LC-MS/MS method. The limit of quantification (LOQ) was set at 50 and 5 ng ml(-1) for the HPLC-UV method and the LC-MS/MS method, respectively. For the UV method, the limit of detection (LOD) was 15 and 10 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs of the LC-MS/MS method in plasma were much lower, i.e. 0.10 and 0.04 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The LODs obtained for the urine samples were 0.16 and 0.09 ng ml(-1) for amiodarone and desethylamiodarone, respectively. The methods were shown to be of use in horses. The rapid HPLC-UV method was used for therapeutic drug monitoring after amiodarone treatment, while the LC-MS/MS method showed its applicability for single dose pharmacokinetic studies.
Publication Date: 2006-04-17 PubMed ID: 16616880DOI: 10.1016/j.jchromb.2006.03.038Google Scholar: Lookup
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Summary
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The study presents a quick method for measuring amiodarone and desethylamiodarone in horse plasma and urine using high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry. It specifically notes the suitability of these methods for therapeutic drug monitoring and pharmacokinetic studies, respectively in horses.
Research Methods
- The research team utilized a quick method of quantifying amiodarone and desethylamiodarone in animal plasma using high-performance liquid chromatography combined with UV detection (HPLC-UV). This process involved a simple deproteinisation stage with acetonitrile.
- The team also highlighted a sensitive method for quantifying these substances in horse plasma and urine, this time by using high-performance liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The process in this case involved a solid-phase extraction (SPE) with a SCX column.
- In both chromatographic methods, Tamoxifen was used as an internal standard.
Chromatographic Separation
- The process of distinguishing the substances was achieved through utilizing an ODS Hypersil column and isocratic elution.
- For the HPLC-UV method, 0.01% diethylamine and acetonitrile served as the mobile phase, while 0.1% formic acid and acetonitrile were used for the LC-MS/MS method.
Key Findings
- In the HPLC-UV method, good linearity was observed. The limit of quantification (LOQ) and Limit of detection (LOD) for amiodarone and desethylamiodarone were specified.
- Meanwhile, the LODs of the LC-MS/MS method in plasma were much lower. Also, the LODs obtained for urine samples showed finer measurements.
- The study confirmed that these methods are beneficial for uses in horses. The quick HPLC-UV method showed its usability for therapeutic drug monitoring, while the LC-MS/MS method proved suitable for single dose pharmacokinetic studies.
Cite This Article
APA
Maes A, Baert K, Croubels S, De Clercq D, van Loon G, Deprez P, De Backer P.
(2006).
Determination of amiodarone and desethylamiodarone in horse plasma and urine by high-performance liquid chromatography combined with UV detection and electrospray ionization mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci, 836(1-2), 47-56.
https://doi.org/10.1016/j.jchromb.2006.03.038 Publication
Researcher Affiliations
- Department of Pharmacology, Toxicology, Biochemistry and Organ Physiology, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. an.maes@ugent.be
MeSH Terms
- Amiodarone / analogs & derivatives
- Amiodarone / blood
- Amiodarone / urine
- Animals
- Chromatography, High Pressure Liquid / methods
- Horses
- Reference Standards
- Sensitivity and Specificity
- Spectrometry, Mass, Electrospray Ionization / methods
- Spectrophotometry, Ultraviolet / methods
Citations
This article has been cited 1 times.- Elgart A, Cherniakov I, Aldouby Y, Domb AJ, Hoffman A. Improved oral bioavailability of BCS class 2 compounds by self nano-emulsifying drug delivery systems (SNEDDS): the underlying mechanisms for amiodarone and talinolol.. Pharm Res 2013 Dec;30(12):3029-44.
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