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Home / Equine Research Bank / J Vet Sci / Determination of angiotensin I-converting enzyme activity in...
Journal of veterinary science2011; 12(1); 21-25; doi: 10.4142/jvs.2011.12.1.21

Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis.

Abstract: Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloylphenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays.
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Publication Date: 2011-03-04 PubMed ID: 21368559PubMed Central: PMC3053463DOI: 10.4142/jvs.2011.12.1.21Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the agreement between different methods used to measure angiotensin-I converting enzyme (ACE) activity in horse blood, a potential biomarker of athletic performance. The results show a lack of consensus between the methods and stress the importance of using a single method of analysis for comparability.

Research Background and Purpose

  • The article begins by highlighting the role of Angiotensin-I converting enzyme (ACE) in regulating blood pressure, fluid homeostasis, and electrolytes by converting angiotensin I into angiotensin II.
  • A recent genetic study suggests that a polymorphism of the ACE gene accounts for almost half of the variation in ACE activity, pointing towards its potential use as a marker of athletic aptitude.
  • The authors point out that human and equine research often uses different methods to determine ACE activity, without a universally accepted “gold standard”. Despite being unsanctioned as comparable, these tests have often been used interchangeably.
  • The objective of this study is to quantify the level of agreement between three different tests when analyzing equine plasma.

Study Methodology

  • The study uses plasma samples from 29 horses and examines their ACE activity using three distinct assays. Two of these are spectrophotometric assays that employ the compound Furylacryloylphenylalanyl-glycyl-glycine as a substrate, and one fluorimetric assay using a compound called o-aminobenzoic acid-FRK-(Dnp)P-OH.

Findings

  • The authors found that there is significant disagreement in the results produced by these different methods for measuring ACE activity. The implication of this finding is that these methods cannot and should not be used interchangeably for equivalent results.
  • For comparable data, the authors advocate choosing and sticking to a single method for all analyses.
  • They also highlight the fact that the literature needs thoughtful appraisal when comparing results obtained from different assays.

Cite This Article

APA
Costa MF, Carmona AK, Alves MF, Ryan TM, Davies HM, Anderson GA, Slocombe RF. (2011). Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis. J Vet Sci, 12(1), 21-25. https://doi.org/10.4142/jvs.2011.12.1.21

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 12
Issue: 1
Pages: 21-25

Researcher Affiliations

Costa, Maria Fernanda de M
  • Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. Fernanda.Costa@wintec.ac.nz
Carmona, Adriana K
    Alves, Marcio F M
      Ryan, Timothy M
        Davies, Helen M
          Anderson, Garry A
            Slocombe, Ron F

              MeSH Terms

              • Animals
              • Enzyme Assays / methods
              • Female
              • Fluorometry / methods
              • Horses / blood
              • Horses / genetics
              • Horses / metabolism
              • Male
              • Oligopeptides / pharmacology
              • Peptidyl-Dipeptidase A / blood
              • Peptidyl-Dipeptidase A / genetics
              • Peptidyl-Dipeptidase A / metabolism
              • Polymorphism, Genetic
              • Reference Values
              • Spectrophotometry / methods

              References

              This article includes 30 references
              1. Alves MF, Araujo MC, Juliano MA, Oliveira EM, Krieger JE, Casarini DE, Juliano L, Carmona AK. A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity.. Braz J Med Biol Res 2005 Jun;38(6):861-8.
                pubmed: 15933779doi: 10.1590/s0100-879x2005000600007google scholar: lookup
              2. Araujo MC, Melo RL, Cesari MH, Juliano MA, Juliano L, Carmona AK. Peptidase specificity characterization of C- and N-terminal catalytic sites of angiotensin I-converting enzyme.. Biochemistry 2000 Jul 25;39(29):8519-25.
                pubmed: 10913258doi: 10.1021/bi9928905google scholar: lookup
              3. Ball BA, Gravance CG, Wessel MT, Sabeur K. Activity of angiotensin-converting enzyme (ACE) in reproductive tissues of the stallion and effects of angiotensin II on sperm motility.. Theriogenology 2003 Feb;59(3-4):901-14.
                pubmed: 12517392doi: 10.1016/s0093-691x(02)01127-5google scholar: lookup
              4. . The effect of benazepril on survival times and clinical signs of dogs with congestive heart failure: Results of a multicenter, prospective, randomized, double-blinded, placebo-controlled, long-term clinical trial.. J Vet Cardiol 1999 May;1(1):7-18.
                pubmed: 19081317doi: 10.1016/s1760-2734(06)70025-xgoogle scholar: lookup
              5. Beneteau B, Baudin B, Morgant G, Giboudeau J, Baumann FC. Automated kinetic assay of angiotensin-converting enzyme in serum.. Clin Chem 1986 May;32(5):884-6.
                pubmed: 3009054
              6. Bland JM, Altman DG. Measuring agreement in method comparison studies.. Stat Methods Med Res 1999 Jun;8(2):135-60.
                pubmed: 10501650doi: 10.1177/096228029900800204google scholar: lookup
              7. Bramucci M, Miano A, Quassinti L, Maccari E, Murri O, Amici D. Presence and comparison of angiotensin converting enzyme in commercial cell culture sera.. Biochem Mol Biol Int 1999 Jan;47(1):107-15.
                pubmed: 10092950doi: 10.1080/15216549900201103google scholar: lookup
              8. Carmona AK, Schwager SL, Juliano MA, Juliano L, Sturrock ED. A continuous fluorescence resonance energy transfer angiotensin I-converting enzyme assay.. Nat Protoc 2006;1(4):1971-6.
                pubmed: 17487185doi: 10.1038/nprot.2006.306google scholar: lookup
              9. Coomer RR, Forhead AJ, Bathe AP, Head MJ. Plasma angiotensin-converting enzyme (ACE) concentration in Thoroughbred racehorses.. Equine Vet J 2003 Jan;35(1):96-8.
                pubmed: 12553471doi: 10.2746/042516403775467487google scholar: lookup
              10. Costa MFM. Angiotensin-converting enzyme (ACE) in horses and its relationship to performance and fitness. Ph.D dissertation. Melbourne: The University of Melbourne; 2010.
              11. Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung.. Biochem Pharmacol 1971 Jul;20(7):1637-48.
                pubmed: 4355305doi: 10.1016/0006-2952(71)90292-9google scholar: lookup
              12. Ellis N. Molecular genetic characterization of the equine angiotensin-converting enzyme gene. Faculty of Veterinary Science. Sydney: The University of Sydney; 2005. p. 338.
              13. Fogarty Y, Fraser CG, Browning MC. Intra- and inter-individual variation of serum angiotensin converting enzyme: clinical implications.. Ann Clin Biochem 1989 Mar;26 ( Pt 2):201-2.
                pubmed: 2543259doi: 10.1177/000456328902600224google scholar: lookup
              14. Forhead AJ, Gillespie CE, Fowden AL. Role of cortisol in the ontogenic control of pulmonary and renal angiotensin-converting enzyme in fetal sheep near term.. J Physiol 2000 Jul 15;526 Pt 2(Pt 2):409-16.
                pmc: PMC2270017pubmed: 10896729doi: 10.1111/j.1469-7793.2000.00409.xgoogle scholar: lookup
              15. Ganjam VK, Evans TJ. Equine endometrial fibrosis correlates with 11beta-HSD2, TGF-beta1 and ACE activities.. Mol Cell Endocrinol 2006 Mar 27;248(1-2):104-8.
                pubmed: 16406651doi: 10.1016/j.mce.2005.12.008google scholar: lookup
              16. Gardner SY, Atkins CE, Sams RA, Schwabenton AB, Papich MG. Characterization of the pharmacokinetic and pharmacodynamic properties of the angiotensin-converting enzyme inhibitor, enalapril, in horses.. J Vet Intern Med 2004 Mar-Apr;18(2):231-7.
                pubmed: 15058776doi: 10.1892/0891-6640(2004)18<231:cotpap>2.0.co;2google scholar: lookup
              17. Hurst PL, Lovell-Smith CJ. Optimized assay for serum angiotensin-converting enzyme activity.. Clin Chem 1981 Dec;27(12):2048-52.
                pubmed: 6273013
              18. Mantha S, Roizen MF, Fleisher LA, Thisted R, Foss J. Comparing methods of clinical measurement: reporting standards for bland and altman analysis.. Anesth Analg 2000 Mar;90(3):593-602.
                pubmed: 10702443doi: 10.1097/00000539-200003000-00018google scholar: lookup
              19. Montgomery H, Clarkson P, Barnard M, Bell J, Brynes A, Dollery C, Hajnal J, Hemingway H, Mercer D, Jarman P, Marshall R, Prasad K, Rayson M, Saeed N, Talmud P, Thomas L, Jubb M, World M, Humphries S. Angiotensin-converting-enzyme gene insertion/deletion polymorphism and response to physical training.. Lancet 1999 Feb 13;353(9152):541-5.
                pubmed: 10028982doi: 10.1016/s0140-6736(98)07131-1google scholar: lookup
              20. Muir WW 3rd, Sams RA, Hubbell JA, Hinchcliff KW, Gadawski J. Effects of enalaprilat on cardiorespiratory, hemodynamic, and hematologic variables in exercising horses.. Am J Vet Res 2001 Jul;62(7):1008-13.
                pubmed: 11453472doi: 10.2460/ajvr.2001.62.1008google scholar: lookup
              21. Myerson S, Hemingway H, Budget R, Martin J, Humphries S, Montgomery H. Human angiotensin I-converting enzyme gene and endurance performance.. J Appl Physiol (1985) 1999 Oct;87(4):1313-6.
                pubmed: 10517757doi: 10.1152/jappl.1999.87.4.1313google scholar: lookup
              22. Nocenti MR, Simchon S, Cizek LJ. Analysis of the renin-angiotensin system during fasting in adult male rabbits.. Proc Soc Exp Biol Med 1975 Oct;150(1):142-7.
                pubmed: 171680doi: 10.3181/00379727-150-38991google scholar: lookup
              23. O'Connor SJ, Fowden AL, Holdstock N, Giussani DA, Forhead AJ. Developmental changes in pulmonary and renal angiotensin-converting enzyme concentration in fetal and neonatal horses.. Reprod Fertil Dev 2002;14(7-8):413-7.
                pubmed: 12617784doi: 10.1071/rd02044google scholar: lookup
              24. Raimbach SJ, Thomas AL. Renin and angiotensin converting enzyme concentrations in the fetal and neonatal guinea-pig.. J Physiol 1990 Apr;423:441-51.
                pmc: PMC1189767pubmed: 2167364doi: 10.1113/jphysiol.1990.sp018032google scholar: lookup
              25. Rigat B, Hubert C, Alhenc-Gelas F, Cambien F, Corvol P, Soubrier F. An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels.. J Clin Invest 1990 Oct;86(4):1343-6.
                pmc: PMC296868pubmed: 1976655doi: 10.1172/jci114844google scholar: lookup
              26. Ronca-Testoni S. Direct spectrophotometric assay for angiotensin-converting enzyme in serum.. Clin Chem 1983 Jun;29(6):1093-6.
                pubmed: 6303627
              27. Sabatini RA, Bersanetti PA, Farias SL, Juliano L, Juliano MA, Casarini DE, Carmona AK, Paiva AC, Pesquero JB. Determination of angiotensin I-converting enzyme activity in cell culture using fluorescence resonance energy transfer peptides.. Anal Biochem 2007 Apr 15;363(2):255-62.
                pubmed: 17320031doi: 10.1016/j.ab.2007.01.020google scholar: lookup
              28. Schweisfurth H, Schiöberg-Schiegnitz S. Assay and biochemical characterization of angiotensin-I-converting enzyme in cerebrospinal fluid.. Enzyme 1984;32(1):12-9.
                pubmed: 6090116doi: 10.1159/000469445google scholar: lookup
              29. Tillman LG, Moore JN. Serum angiotensin converting enzyme activity and response to angiotensin I in horses.. Equine Vet J Suppl 1989 Jun;(7):80-3.
                pubmed: 9118114doi: 10.1111/j.2042-3306.1989.tb05662.xgoogle scholar: lookup
              30. Tiret L, Rigat B, Visvikis S, Breda C, Corvol P, Cambien F, Soubrier F. Evidence, from combined segregation and linkage analysis, that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels.. Am J Hum Genet 1992 Jul;51(1):197-205.
                pmc: PMC1682892pubmed: 1319114

              Citations

              This article has been cited 2 times.
              1. Li CC, Lee YC, Lo HY, Huang YW, Hsiang CY, Ho TY. Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition.. Molecules 2019 May 16;24(10).
                doi: 10.3390/molecules24101886pubmed: 31100914google scholar: lookup
              2. Robati RM, Ayatollahi A, Toossi P, Younespour S. Serum Angiotensin converting enzyme in pemphigus vulgaris.. Indian J Dermatol 2014 Jul;59(4):348-51.
                doi: 10.4103/0019-5154.135478pubmed: 25071251google scholar: lookup
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