Determination of glucosamine in horse plasma by liquid chromatography tandem mass spectrometry.
Abstract: Glucosamine is an amino sugar involved in the biosynthesis of glycosylated proteins and lipids. Recently, with increased public interest in natural products medicine, glucosamine has been widely used to treat osteoarthritis, even though demonstrations of its actual efficacy remain relatively unknown. Information related to the pharmcokinetics of glucosamine is sparse. A recent analytical method published used 13C-glucosamine as an internal standard to analyse study samples. The method lacked accuracy owing to an important natural isotopic contribution of glucosamine to 13C-glucosamine ion abundance. This manuscript describes a simple method to quantify glucosamine in horse plasma. Glucosamine was extracted by protein precipitation with acetonotrile containing 0.1% formic acid. The chromatography was performed on a Agilent Hypersil-ODS 100x2.1 mm column with a mobile phase composed of acetonitrile and 0.5% formic acid in water (45:55) at a flow rate of 0.3 mL/min. A linear (1/x) relationship was used to perform the calibration over an analytical range of 10-1000 ng/mL. The inter-batch precision and accuracy ranged from 5.3 to 11.3% and from 87.8 to 107.2% in horse plasma, respectively. The mean endogenous level of glucosamine in horse plasma was 14.4 ng/mL (n=6). This LC-ESI/MS/MS method for the determination of glucosamine in horse plasma provided results within generally accepted criteria used for bioanalytical assay.
Copyright (c) 2007 John Wiley & Sons, Ltd.
Publication Date: 2007-09-13 PubMed ID: 17849505DOI: 10.1002/bmc.906Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Evaluation Study
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The researchers developed a simple method to measure glucosamine, a compound used in osteoarthritis treatment, in horse plasma using liquid chromatography tandem mass spectrometry.
Research Purpose
- The aim of the study was to design an accurate and simple method to quantify the amount of glucosamine in horse plasma. This was in response to existing methods which had some flaws in accuracy.
Methods and Experimentation
- The researchers extracted glucosamine from horse plasma by precipitating the protein with acetonitrile that had 0.1% formic acid.
- A Agilent Hypersil-ODS 100×2.1 mm column was used to perform the chromatography.
- The flow rate for the mobile phase, composed of acetonitrile and 0.5% formic acid in water (45:55), was maintained at 0.3 mL/min.
- The team calibrated the amount of glucosamine using a linear (1/x) relationship, over a range of 10-1000 ng/mL.
Results
- The precision and accuracy ranged from 5.3 to 11.3% and from 87.8 to 107.2%, respectively, in horse plasma.
- The mean endogenous level of glucosamine in horse plasma measured was 14.4 ng/mL.
- Applying criteria typically used for bioanalytical assays, the results from this LC- ESI/MS/MS method for determining glucosamine in horse plasma were found to be within generally accepted limits.
Significance
- This research provides an improved method to accurately quantify glucosamine in horse plasma.
- The method may enhance our understanding of glucosamine, a compound widely used to treat osteoarthritis, by providing more accurate information about its pharmacokinetics.
Cite This Article
APA
Beaudry F, Vachon P.
(2007).
Determination of glucosamine in horse plasma by liquid chromatography tandem mass spectrometry.
Biomed Chromatogr, 22(1), 1-4.
https://doi.org/10.1002/bmc.906 Publication
Researcher Affiliations
- Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, St-Hyacinthe, Q, Canada J2S 2M2. Francis.beaudry@umontreal.ca
MeSH Terms
- Acetonitriles / chemistry
- Animals
- Carbon Isotopes
- Chromatography, High Pressure Liquid / instrumentation
- Chromatography, High Pressure Liquid / methods
- Formates / chemistry
- Glucosamine / blood
- Horses
- Reference Standards
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Mass, Electrospray Ionization / instrumentation
- Spectrometry, Mass, Electrospray Ionization / methods
- Tandem Mass Spectrometry / instrumentation
- Tandem Mass Spectrometry / methods
- Theophylline / standards
Citations
This article has been cited 2 times.- Song M, Hang TJ, Wang C, Yang L, Wen AD. Precolumn derivatization LC-MS/MS method for the determination and pharmacokinetic study of glucosamine in human plasma and urine. J Pharm Anal 2012 Feb;2(1):19-28.
- Schwarz MCR, Moskaluk AE, Daniels JB, VandeWoude S, Reynolds MM. Current Analytical Methods and Challenges for the Clinical Diagnosis of Invasive Pulmonary Aspergillosis Infection. J Fungi (Basel) 2024 Nov 28;10(12).
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
