Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry.
Abstract: The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC-electrospray-ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This "top-down" approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples.
Publication Date: 2008-02-15 PubMed ID: 18274736DOI: 10.1007/s00216-008-1889-zGoogle Scholar: Lookup
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- Journal Article
Summary
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The research article presents a new method to detect the presence of the Insulin-like-growth factor (IGF-I) in horse plasma. This was achieved through the development of software combined with HPLC-electrospray-ion-trap mass spectrometry and product-ion scan experiments.
Objective of the Research
- The primary objective of the research was to develop a method for both the quantitative and qualitative analysis of IGF-I in horse plasma samples. IGF-I is considered a significant indirect indicator of growth hormone administration in horses. Hence, accurately measuring it could provide vital information about the horse’s health and growth condition.
Development of a Protein-Transposing Software
- The researchers developed novel protein-transposing software to simplify the process of quantifying IGF-I. The software was designed to work in conjunction with high-performance liquid chromatography (HPLC) electrospray ion-trap mass spectrometry. This technique offers high sensitivity and precision, making it an excellent tool for detecting and measuring IGF-I.
Product-Ion Scan Experiments
- Alongside the development of the software, product-ion scan experiments were conducted to validate the software and technique’s effectiveness. The researchers tested standard samples, non-fortified equine plasma samples, fortified plasma samples, and plasma samples taken post-administration of equine growth hormone.
- The results from these tests allowed the researchers to confirm that this “top-down” approach could successfully identify IGF-I. This method enabled the characterization of fragment ions that correspond to the carboxy terminal end of IGF-I, which is very useful for confirming its presence in plasma samples.
Significance of the Study
- This research presents a significant step forward in veterinary science and equine health management. The ability to accurately detect and measure IGF-I in horse plasma will allow veterinarians and horse owners to better manage and monitor horse health, particularly in relation to growth hormone administration.
Cite This Article
APA
Popot MA, Woolfitt AR, Garcia P, Tabet JC.
(2008).
Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry.
Anal Bioanal Chem, 390(7), 1843-1852.
https://doi.org/10.1007/s00216-008-1889-z Publication
Researcher Affiliations
- LCH, Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370, Verrières le Buisson, France. mariepopot@wanadoo.fr.
MeSH Terms
- Animals
- Chromatography, Liquid / methods
- Horses
- Insulin-Like Growth Factor I / analysis
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Mass, Electrospray Ionization / methods
Citations
This article has been cited 3 times.- Cowan DA, Moncrieffe DA. Procollagen type III amino-terminal propeptide and insulin-like growth factor I as biomarkers of growth hormone administration. Drug Test Anal 2022 May;14(5):808-819.
- Baskerville CL, Bamford NJ, Harris PA, Bailey SR. Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse. Open Vet J 2017;7(1):75-80.
- Kam RK, Ho CS, Chan MH. Serum Insulin-like Growth Factor I Quantitation by Mass Spectrometry: Insights for Protein Quantitation with this Technology. EJIFCC 2016 Dec;27(4):318-330.
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