[Determination of immunoglobulin G in mare colostrum by high-performance gel permeation chromatography].

The research article is about a study developing a direct high-performance gel permeation chromatographic (HPGPC) method to measure immunoglobulin G in mare colostrum to provide high accuracy and precision.

Methodology

• For the determination of immunoglobulin G (IgG) in mare colostrum, researchers employed a direct high-performance gel permeation chromatographic (HPGPC) method.
• This chromatographic separation was executed on a TOSOH TSK-G4000PW(XL) column with specific dimensions and phosphate buffer solution as the mobile phase at a controlled flow rate.
• The column temperature was strictly maintained, the injection volume was set at 20 microL, and the detection wavelength was at 280 nm.
• The linear range was defined between 0.2 to 3.0 g/L, having a detection limit of 0.08 mg/L. A recovery rate of approximately 97% was achieved with a minor relative standard deviation.
• The stability, accuracy, and reproducibility of the established method were assessed and found to be reliable within acceptable percentage ranges.
• Mare milk was collected from a location in China, preserved in an ice box, sent to the laboratory, and subsequently stored in a low-temperature refrigerator.
• The whey milk was prepared via a specific centrifugation procedure, and the whey protein was obtained from the middle layer.

Results and Conclusion

• When a portion of the whey milk was mixed with the mobile phase, the average content of IgG ranged between 35.0 g/L to 50.0 g/L during the first lactation period.
• This IgG content dropped to 2.0 g/L to 4.0 g/L after 72 hours.
• The analytical method utilized was verified to be both accurate and precise for application to mare colostrum, making it a reliable process for determining IgG content in this fluid.