Determination of lactate concentrations in blood plasma and peritoneal fluid in horses with colic by an Accusport analyzer.
Abstract: Intestinal hypoperfusion can lead to increased lactate concentrations in plasma and peritoneal fluid of horses with colic. Objective: The purposes of this study were to (1) evaluate the reliability of the Accusport analyzer to assess peritoneal fluid lactate (PFL) concentrations in healthy horses and those with colic, (2) identify clinical features associated with abnormal blood plasma lactate (BPL) and PFL concentrations, and (3) evaluate the prognostic value of BPL and PFL. Methods: BPL and PFL were determined in 20 healthy horses and in 106 horses with colic. Results: The Accusport was reliable for determining BPL concentrations < 13 mM and PFL concentrations < 20 mM. Multivariate analysis indicated that PCV and the need for intestinal resection were independently associated with the BPL; pulse, PCV, venous pO2, the presence of necrotic intestine, an increased amount of peritoneal fluid, and fluid total protein content were independently associated with PFL. With a 1 mM increase in BPL or PFL, the respective odds ratios for required abdominal surgery increase to 1.23 (BPL) and 1.58 (PFL), odds ratios for a required intestinal resection increase to 1.20 (BPL) and 1.41 (PFL), and odds ratios for developing ileus increase by 1.33 (BPL) and 1.36 (PFL). PFL concentrations of 1, 6, 12, and 16 mM correspond to a probability of death of 11, 29, 63, and 82%, respectively, in horses without strangulating obstruction and of 25, 52, 82, and 92%, respectively, in horses with strangulating obstruction. Conclusions: PFL is more useful and sensitive than BPL for prognostic purposes in horses with colic.
Publication Date: 2007-04-13 PubMed ID: 17427391DOI: 10.1892/0891-6640(2007)21[293:dolcib]2.0.co;2Google Scholar: Lookup
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- Journal Article
Summary
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The research studied the reliability of the Accusport analyzer in measuring lactate concentrations in blood and peritoneal fluid in healthy horses versus those with colic. It also identified clinical features connected to abnormal lactate concentrations and evaluated the prognostic value of these measurements. The Accusport analyzer was found to be reliable and pointed to increased chances of needed surgery and death with growing lactate concentrations, particularly in the peritoneal fluid.
Reliability of Accusport Analyzer
- The study first focused on determining the reliability of the Accusport analyzer for measuring lactate concentrations in the blood plasma and peritoneal fluid of horses.
- The experiment was first conducted on 20 healthy horses, and then on 106 horses suffering from colic.
- Findings indicated that the analyzer could reliably determine blood plasma lactate (BPL) concentrations less than 13 mM and peritoneal fluid lactate (PFL) concentrations less than 20 mM.
Identifying Clinical Associations
- The study next turned to identifying clinical features associated with abnormal BPL and PFL concentrations in horses.
- Through multivariate analysis, it was identified that PCV (Packed Cell Volume) and the need for intestinal resection were independently linked to BPL. Conversely, pulse, PCV, venous pO2 (partial pressure of Oxygen), the presence of necrotic intestine, increased amount of peritoneal fluid, and fluid total protein content were associated with PFL.
Evaluating Prognostic Value
- The research further evaluated the prognostic value of BPL and PFL in determining the outcome for horses with colic.
- Findings indicated that every 1 mM increase in BPL or PFL significantly increased the odds ratios for required abdominal surgery, intestinal resection, and the development of ileus (a disruption of the normal propulsive ability of the gastrointestinal tract).
- Increased PFL concentrations, especially, were associated with a high probability of death in horses with colic, a higher value signifying a higher risk. The study concluded that PFL proves more sensitive than BPL for prognostic purposes in horses with colic.
Cite This Article
APA
Delesalle C, Dewulf J, Lefebvre RA, Schuurkes JA, Proot J, Lefere L, Deprez P.
(2007).
Determination of lactate concentrations in blood plasma and peritoneal fluid in horses with colic by an Accusport analyzer.
J Vet Intern Med, 21(2), 293-301.
https://doi.org/10.1892/0891-6640(2007)21[293:dolcib]2.0.co;2 Publication
Researcher Affiliations
- Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Catherine.Delesalle@UGent.be
MeSH Terms
- Animals
- Ascitic Fluid / chemistry
- Biomarkers / analysis
- Biomarkers / blood
- Blood Chemical Analysis / instrumentation
- Blood Chemical Analysis / veterinary
- Colic / metabolism
- Colic / veterinary
- Female
- Horse Diseases / metabolism
- Horses
- Lactates / analysis
- Lactates / blood
- Male
Citations
This article has been cited 10 times.- Ludwig EK, Hobbs KJ, McKinney-Aguirre CA, Gonzalez LM. Biomarkers of Intestinal Injury in Colic.. Animals (Basel) 2023 Jan 7;13(2).
- Martín-Cuervo M, Gracia-Calvo LA, Macías-García B, Ezquerra LJ, Barrera R. Evaluation of Eosinopenia as a SIRS Biomarker in Critically Ill Horses.. Animals (Basel) 2022 Dec 15;12(24).
- Smanik LE, Moser DK, Rothers KP, Hackett ES. Serial venous lactate measurement following gastrointestinal surgery in horses.. J Vet Sci 2022 Sep;23(5):e66.
- Bishop RC, Gutierrez-Nibeyro SD, Stewart MC, McCoy AM. Performance of predictive models of survival in horses undergoing emergency exploratory laparotomy for colic.. Vet Surg 2022 Aug;51(6):891-902.
- Dybkjær E, Steffensen KF, Honoré ML, Dinesen MA, Christophersen MT, Pihl TH. Short-term survival rates of 1397 horses referred for colic from 2010 to 2018.. Acta Vet Scand 2022 May 7;64(1):11.
- Barton AK, Richter IG, Ahrens T, Merle R, Alalwani A, Lilge S, Purschke K, Barnewitz D, Gehlen H. MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic.. Mediators Inflamm 2021;2021:9501478.
- Witkowska-Piłaszewicz O, Cywińska A, Michlik-Połczyńska K, Czopowicz M, Strzelec K, Biazik A, Parzeniecka-Jaworska M, Crisman M, Witkowski L. Variations in haematological and biochemical parameters in healthy ponies.. BMC Vet Res 2021 Jan 19;17(1):38.
- Cole GC, Tordiffe ASW, Steenkamp G. Assessment of a portable lactate meter for field use in the white rhinoceros (Ceratotherium simum).. Onderstepoort J Vet Res 2017 Nov 10;84(1):e1-e10.
- Murakami T, Nakao S, Sato Y, Nakada S, Sato A, Mukai S, Kobayashi M, Yamada Y, Kawakami E. Blood lactate concentration as diagnostic predictors of uterine necrosis and its outcome in dairy cows with uterine torsion.. J Vet Med Sci 2017 Mar 18;79(3):513-516.
- Stoot LJ, Cairns NA, Cull F, Taylor JJ, Jeffrey JD, Morin F, Mandelman JW, Clark TD, Cooke SJ. Use of portable blood physiology point-of-care devices for basic and applied research on vertebrates: a review.. Conserv Physiol 2014;2(1):cou011.
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