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Veterinary parasitology2000; 92(2); 165-169; doi: 10.1016/s0304-4017(00)00280-6

Determination of the activity of ponazuril against Sarcocystis neurona in cell cultures.

Abstract: The present study examined the efficacy of ponazuril in inhibiting merozoite production of Sarcocystis neurona in cell cultures. Ponazuril inhibited merozoite production by more that 90% in cultures of S. neurona treated with 1.0 microg/ml ponazuril and greater than 95% inhibition of merozoite production was observed when infected cultures were treated with 5.0 microg/ml ponazuril. Ponazuril may have promise as a therapeutic agent in the treatment of S. neurona induced equine protozoal myeloencephalitis (EPM) in horses.
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Publication Date: 2000-08-18 PubMed ID: 10946140DOI: 10.1016/s0304-4017(00)00280-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research conducted tests on the effectiveness of ponazuril in stopping the reproduction of Sarcocystis neurona, a parasite, in cell cultures. It was found that ponazuril was highly successful in preventing the production of new parasites, and thus could potentially be useful for treating the infection in horses.

Examination of Ponazuril’s Efficacy

The study’s primary objective was to test the capacity of ponazuril, a drug commonly used to combat protozoal infections, to impede the multiplication of Sarcocystis neurona. This objective forms the core of the research.

  • The researchers examined the effectiveness of ponazuril against the creation of merozoites, a form of the parasite responsible for its replication.
  • The experiment was conducted in cell cultures infected with Sarcocystis neurona.
  • The ability of ponazuril to inhibit merozoite creation was closely observed and measured.

Results of the Study

The paper’s findings suggested a promising potential for ponazuril in the treatment of S. neurona infections.

  • In the cultures treated with 1.0 microg/ml ponazuril, merozoite production was halted by more than 90%.
  • Increased effectiveness was observed in cultures treated with a higher dose of the drug. Specifically, more than 95% inhibition of merozoite production occurred in cultures treated with 5.0 microg/ml ponazuril.
  • These results suggest a direct correlation between the concentration of ponazuril and its inhibitory effect on merozoite production.

Potential Treatment for Equine Protozoal Myeloencephalitis

Equine protozoal myeloencephalitis (EPM) is a severe neurological disease in horses caused by S. neurona. The research’s results indicate a potential for ponazuril in treating EPM.

  • As ponazuril demonstrated substantial effectiveness in inhibiting the multiplication of S. neurona in cell cultures, it was suggested as a promising therapeutic agent against the condition.
  • The authors propose further exploration into the use of ponazuril in the treatment of EPM based on the findings of the study.

Cite This Article

APA
Lindsay DS, Dubey JP, Kennedy TJ. (2000). Determination of the activity of ponazuril against Sarcocystis neurona in cell cultures. Vet Parasitol, 92(2), 165-169. https://doi.org/10.1016/s0304-4017(00)00280-6

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 92
Issue: 2
Pages: 165-169

Researcher Affiliations

Lindsay, D S
  • Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, 24061-0342, USA. lindsayd@vt.edu
Dubey, J P
    Kennedy, T J

      MeSH Terms

      • Animals
      • Antiprotozoal Agents / pharmacology
      • Antiprotozoal Agents / therapeutic use
      • Cell Line
      • Encephalomyelitis / drug therapy
      • Encephalomyelitis / parasitology
      • Encephalomyelitis / veterinary
      • Horse Diseases / drug therapy
      • Horse Diseases / parasitology
      • Horses
      • Sarcocystis / drug effects
      • Sarcocystis / growth & development
      • Sarcocystosis / drug therapy
      • Sarcocystosis / veterinary
      • Triazines / pharmacology
      • Triazines / therapeutic use

      Citations

      This article has been cited 11 times.
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        doi: 10.3389/fvets.2022.1054417pubmed: 36570513google scholar: lookup
      2. Jacobson ER, Stacy NI, Mader DR, Moretti R, Zirkelbach B, Carlile O, O'Connor CD, Caperton KJ, Maxwell LK. Pharmacokinetics of ponazuril after administration of a single oral dose to green turtles (Chelonia mydas). Vet Q 2021 Dec;41(1):323-331.
        doi: 10.1080/01652176.2021.2008045pubmed: 34789079google scholar: lookup
      3. Furr M, Kennedy T. Effects of coadministration of corn oil and ponazuril on serum and cerebrospinal fluid concentrations of ponazuril in horses. J Vet Intern Med 2020 May;34(3):1321-1324.
        doi: 10.1111/jvim.15765pubmed: 32301131google scholar: lookup
      4. Bowden GD, Land KM, O'Connor RM, Fritz HM. High-throughput screen of drug repurposing library identifies inhibitors of Sarcocystis neurona growth. Int J Parasitol Drugs Drug Resist 2018 Apr;8(1):137-144.
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      5. Reed SM, Furr M, Howe DK, Johnson AL, MacKay RJ, Morrow JK, Pusterla N, Witonsky S. Equine Protozoal Myeloencephalitis: An Updated Consensus Statement with a Focus on Parasite Biology, Diagnosis, Treatment, and Prevention. J Vet Intern Med 2016 Mar-Apr;30(2):491-502.
        doi: 10.1111/jvim.13834pubmed: 26857902google scholar: lookup
      6. Dubey JP, Howe DK, Furr M, Saville WJ, Marsh AE, Reed SM, Grigg ME. An update on Sarcocystis neurona infections in animals and equine protozoal myeloencephalitis (EPM). Vet Parasitol 2015 Apr 15;209(1-2):1-42.
        doi: 10.1016/j.vetpar.2015.01.026pubmed: 25737052google scholar: lookup
      7. Maier K, Olias P, Gruber AD, Lierz M. Toltrazuril does not show an effect against pigeon protozoal encephalitis. Parasitol Res 2015 Apr;114(4):1603-6.
        doi: 10.1007/s00436-015-4342-1pubmed: 25648444google scholar: lookup
      8. Billeter SA, Spencer JA, Chobotar B, Blagburn BL. Ponazuril inhibits the development of Eimeria vermiformis in experimentally infected outbred Swiss mice. Parasitol Res 2005 Feb;95(3):172-5.
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      9. Sun J, Sun Y, Gu YC, Lin JH, Xiao JC. Barton-Kellogg Olefination of (CF(3)S)(2)C=S and Subsequent Cyclopropanation for the Installation of Bulky Bis(trifluoromethylthio)methylene Group. JACS Au 2025 Feb 24;5(2):1039-1050.
        doi: 10.1021/jacsau.4c01270pubmed: 40017769google scholar: lookup
      10. Weerarathne P, Reichard M, Miller C, Scimeca RC. The Establishment of a Novel In Vitro System for Culturing Cytauxzoon felis. Pathogens 2024 Jul 4;13(7).
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      11. Park H, Kim E, Lee TH, Park S, Choi JD, Moon G. Multiclass Method for the Determination of Anthelmintic and Antiprotozoal Drugs in Livestock Products by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Food Sci Anim Resour 2023 Sep;43(5):914-937.
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