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Home / Equine Research Bank / Acta Vet Scand / Determination of the unsaturated disaccharides of hyaluronic...
Acta veterinaria Scandinavica2015; 57(1); 12; doi: 10.1186/s13028-015-0098-y

Determination of the unsaturated disaccharides of hyaluronic acid in equine synovial fluid by high-performance liquid chromatography and fluorescence detection.

Abstract: The purpose of this study was to develop and validate an analytical method to determine the presence of hyaluronic acid derived disaccharides in equine synovial fluid. Results: A high-performance liquid chromatography method for the determination of hyaluronic acid derived unsaturated disaccharides in equine synovial fluid was developed and validated. The method is based on the measurement of unsaturated disaccharides released by digestion of linear hyaluronic acid molecules. The method showed linearity (r(2) = 0.996) over the full working concentration range 0.89-30 mg/l. Relative standard deviation of intra- and inter-day precision ranged from of 4.3-6.7% and 7.1-7.8% respectively. The detection limit was 0.3 mg/l corresponding to 20 mg/l in synovial fluid. Accuracy of the assay was 97-103%. This method was evaluated by determining the concentration of unsaturated disaccharides from hyaluronic acid in synovial fluid of horses with lameness in the metacarpo-/metatarsophalangeal joint localized with positive response to intra-articular anesthesia. Conclusions: The described method is valid for determination of hyaluronic acid derived disaccharides in equine synovial fluid. This method was applied to a larger research project dealing with a new form of intra-articular therapy in horses with arthritic diseases.
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Publication Date: 2015-03-04 PubMed ID: 25886740PubMed Central: PMC4350955DOI: 10.1186/s13028-015-0098-yGoogle Scholar: Lookup
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  • Journal Article
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study outlines the successful development and validation of an analytical method to detect hyaluronic acid derived disaccharides in horse synovial fluid – a definitive progress in research related to equine arthritis treatment.

Objectives and Methodology

  • The primary objective of this study was to establish and verify a sophisticated method for detecting the presence of specific disaccharides, derived from hyaluronic acid, in the synovial fluid of horses.
  • The researchers utilized a technique called high-performance liquid chromatography. This approach separates mixtures with high resolution and efficiency, making it ideal for identifying specific molecular entities.
  • The method relied on the measurement of unsaturated disaccharides that are released when linear hyaluronic acid molecules are digested.

Results and Validation

  • This technique yielded accurate and reliable results, with an impressive linearity of 0.996 over the working concentration range of 0.89-30 mg/l.
  • The assay showed consistent results both within and between different days, with a relative standard deviation of 4.3-6.7% for intra-day precision and 7.1-7.8% for inter-day precision, affirming its high level of repeatability.
  • The sensitivity of this method was also satisfactory as the detection limit was identified as 0.3 mg/l, corresponding to 20 mg/l in synovial fluid.
  • The method’s accuracy was between 97% and 103%, rendering the assay extraordinarily reliable.

Application

  • The researchers verified the application of this method by using it to determine the concentration of unsaturated disaccharides from hyaluronic acid in the synovial fluid of lameness-affected horses, specifically those with joint issues in their metacarpo-/metatarsophalangeal joints.
  • These promising results led to the inclusion of this method in a broader research project investigating new forms of intra-articular therapy for equine arthritis.

Conclusion

  • The team confirmed this newly developed method as an effective tool for detecting hyaluronic acid derived disaccharides in equine synovial fluid.
  • It was successful in aiding studies for developing better treatment options for equine arthritis, opening new avenues for further research in this area.

Cite This Article

APA
Aaltonen K, Niemelä T, Sankari S, Tulamo RM. (2015). Determination of the unsaturated disaccharides of hyaluronic acid in equine synovial fluid by high-performance liquid chromatography and fluorescence detection. Acta Vet Scand, 57(1), 12. https://doi.org/10.1186/s13028-015-0098-y

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 57
Issue: 1
Pages: 12
PII: 12

Researcher Affiliations

Aaltonen, Kaisa
  • Department of Equine and Small Animal Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. kaisa.aaltonen@helsinki.fi.
Niemelä, Tytti
  • Department of Equine and Small Animal Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. tytti.niemela@helsinki.fi.
Sankari, Satu
  • Department of Equine and Small Animal Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. satu.sankari@helsinki.fi.
Tulamo, Riitta-Mari
  • Department of Equine and Small Animal Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. riitta.tulamo@helsinki.fi.

MeSH Terms

  • Animals
  • Chromatography, High Pressure Liquid / veterinary
  • Disaccharides / metabolism
  • Horses / metabolism
  • Hyaluronic Acid / metabolism
  • Synovial Fluid / chemistry

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This article includes 19 references
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Citations

This article has been cited 2 times.
  1. Kanyo N, Kovacs KD, Saftics A, Szekacs I, Peter B, Santa-Maria AR, Walter FR, Dér A, Deli MA, Horvath R. Glycocalyx regulates the strength and kinetics of cancer cell adhesion revealed by biophysical models based on high resolution label-free optical data. Sci Rep 2020 Dec 30;10(1):22422.
    doi: 10.1038/s41598-020-80033-6pubmed: 33380731google scholar: lookup
  2. Dai Q, Li Y, Wang M, Li Y, Li J. TlR2 and TlR4 are involved in the treatment of rheumatoid arthritis synovial fibroblasts with a medicated serum of asarinin through inhibition of T(h)1/T(h)17 cytokines. Exp Ther Med 2020 Apr;19(4):3009-3016.
    doi: 10.3892/etm.2020.8557pubmed: 32256787google scholar: lookup
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