Development of a 19-plex short tandem repeat typing system for individual identification and parentage testing of horses (Equus caballus).
Abstract: Individual identification of horses for pedigree verification and registration is important for the sustainable development of the horse industry. Horse individual identification and parentage tests commonly use the 17 short tandem repeats (STRs) recommended by the International Society for Animal Genetics (ISAG) and the locus LEX33. While many multiplex STR typing systems have been established for the horse, a sex determining marker is usually absent, and none of them can simultaneously detect all 17 ISAG recommended loci and the locus LEX33. Here, we present a 19-plex STR typing system that contains the 17 ISAG recommend loci, the locus LEX33 and amelogenin as sex determining loci. The results of our sensitivity, species specificity, stutter analysis and population data analysis, indicate that this system is a specific, sensitive, and robust tool for the identification of individuals, parentage testing and genetic research in the horse.
© 2021 Stichting International Foundation for Animal Genetics.
Publication Date: 2021-07-15 PubMed ID: 34268790DOI: 10.1111/age.13119Google Scholar: Lookup
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Summary
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The study presents a new 19-plex short tandem repeat typing system for identifying individual horses and conducting parentage tests, which includes a sex-determining marker often absent in previous systems.
Understanding Short Tandem Repeat Typing System
- A short tandem repeat (STR) typing system is essentially a DNA profiling technique.
- It involves the examination of short sequences of DNA that repeat in a tandem or consecutive manner.
- The pattern repeats vary among individuals which makes STRs a useful tool for genetic fingerprinting, to identify individuals, and for parental testing.
- The 19-plex STR system described in the study denotes a system that examines 19 different STR patterns in DNA.
Highlighting the Need for STR Typing in Horses
- Identifying individual horses for pedigree verification and registration is crucial for the sustainable growth of the horse industry.
- Current methods for horse identification and parental tests usually involve checking 17 STRs advised by the International Society for Animal Genetics (ISAG) and the locus LEX33.
Presenting the New 19-plex STR Typing System
- The research introduces a new 19-plex STR system which includes the 17 STRs recommended by ISAG, the locus LEX33, and an additional amelogenin as a sex-determining marker.
- This sex-determining marker is typically missing in many multiplex, or having multiple parts, STR typing systems previously established for horses.
- This system, therefore, is unique in being capable of detecting simultaneously all 17 recommended ISAG loci, the locus LEX33, and a sex-determining loci.
Evaluating the New STR Typing System
- The study analyzes the sensitivity, species specificity, stutter analysis — to check any repetitive sequences of DNA that could affect overall patterns — and population data.
- The results indicate that the developed system is specific, sensitive, and robust, proving it a powerful tool for identifying individual horses, conducting parentage tests, and enabling further genetic research.
Cite This Article
APA
Shang S, Jiang R, Luo R, Jia S, Irwin DM, Wang Z, Zhang S.
(2021).
Development of a 19-plex short tandem repeat typing system for individual identification and parentage testing of horses (Equus caballus).
Anim Genet, 52(5), 754-758.
https://doi.org/10.1111/age.13119 Publication
Researcher Affiliations
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
- Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
- Institute of Equine Sciences, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
MeSH Terms
- Alleles
- Animals
- Female
- Gene Frequency
- Horses / genetics
- Male
- Microsatellite Repeats
- Pedigree
- Polymerase Chain Reaction / veterinary
- Species Specificity
Grant Funding
- XLYC1907018 / Department of Education of Liaoning Province
- Organization Department of Liaoning Provincial Committee of China
References
This article includes 20 references
- Bowling AT, Eggleston-Stott ML, Byrns G, Clark RS, Dileanis S, Wictum E. Validation of microsatellite markers for routine horse parentage testing. Animal Genetics 28, 247-52.
- Brookes C, Bright JA, Harbison S, Buckleton J. Characterising stutter in forensic STR multiplexes. Forensic Science International: Genetics 6, 58-63.
- Buckleton JS, Lohmueller KE, Inman K, Cheng K, Curran JM, Pugh SN, Bright JA, Taylor DA. Testing whether stutter and low-level DNA peaks are additive. Forensic Science International: Genetics 43, 102166.
- Chatterji S, Pachter L. Reference based annotation with GeneMapper. Genome Biology 7, R29.
- Chen JW, Uboh CE, Soma LR, Li X, Guan F, You Y, Liu Y. Identification of racehorse and sample contamination by novel 24-plex STR system. Forensic Science International: Genetics 4, 158-67.
- Coogle L, Reid R, Bailey E. Equine dinucleotide repeat loci from LEX025 to LEX033. Animal Genetics 27, 289-90.
- Dimsoski P. Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses. Croatian Medical Journal 44, 332-5.
- Dorji J, Tamang S, Tshewang T, Dorji T, Dorji TY. Genetic diversity and population structure of three traditional horse breeds of Bhutan based on 29 DNA microsatellite markers. PLoS One 13, e0199376.
- Gill P, Evett IW, Woodroffe S, Lygo JE, Millican E, Webster M. Databases, quality control and interpretation of DNA profiling in the Home office Forensic Science Service. Electrophoresis 12, 204-9.
- Gill P, Fereday L, Morling N, Schneider PM. The evolution of DNA databases-recommendations for new European STR loci. Forensic Science International 156, 242-4.
- ISAG. Equine Genetics & Thoroughbred Parentage Testing Standardisation Workshop, Salt Lake, USA. pp. 1-13.
- Jamieson A. The genetics of transferrins in cattle. Heredity 20, 419-41.
- Kun TJ, Wictum EJ, Penedo MCT. A mini-STR typing system for degraded equine DNA. Animal Genetics 49, 464-6.
- Lalitha S. Primer Premier 5. Biotech Software & Internet Report 1, 270-2.
- Lopes MS, Mendonça D, Rojer H, Cabral V, Bettencourt SX, da Câmara MA. Morphological and genetic characterization of an emerging Azorean horse breed: the Terceira Pony. Frontiers in Genetics 6, 62.
- Rousset F. genepop'007: a complete re-implementation of the genepop software for Windows and Linux. Molecular Ecology Resources 8, 103-6.
- Shang S, Zhang M, Zhao Y, Dang W, Hua P, Zhang S, Wang Z. Development and validation of a novel 13-plex PCR system for commonly used short tandem repeats in horses (Equus caballus). Equine Veterinary Journal 51, 688-95.
- Stewart NA, Gerlach RF, Gowland RL, Gron KJ, Montgomery J. Sex determination of human remains from peptides in tooth enamel. Proceedings of the National Academy of Sciences of the United States of America 114, 13649-54.
- van de Goor LHP, Panneman H, van Haeringen WA. A proposal for standardization in forensic equine DNA typing: allele nomenclature for 17 equine-specific STR loci. Animal Genetics 41, 122-7.
- Zhang S, Bian Y, Tian H, Wang Z, Hu Z, Li C. Development and validation of a new STR 25-plex typing system. Forensic Science International: Genetics 17, 61-9.
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