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Journal of veterinary internal medicine2019; 33(5); 2327-2335; doi: 10.1111/jvim.15564

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma.

Abstract: Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance-limiting disease associated with increased allergen-specific immunoglobulin E (IgE) against a range of environmental aeroallergens. Objective: To develop a protein microarray platform to profile IgE against a range of proven and novel environmental proteins in SEA-affected horses. Methods: Six SEA-affected and 6 clinically healthy Warmblood performance horses. Methods: Developed a protein microarray (n = 384) using protein extracts and purified proteins from a large number of families including pollen, bacteria, fungi, and arthropods associated with the horses, environment. Conditions were optimized and assessed for printing, incubation, immunolabeling, biological fluid source, concentration techniques, reproducibility, and specificity. Results: This method identified a number of novel allergens, while also identifying an association between SEA and pollen sensitization. Immunolabeling methods confirmed the accuracy of a commercially available mouse anti-horse IgE 3H10 source (R = 0.91). Biological fluid source evaluation indicated that sera and bronchoalveolar lavage fluid (BALF) yielded the same specific IgE profile (average R = 0.75). Amicon centrifugal filters were found to be the most efficient technique for concentrating BALF for IgE analysis at 40-fold. Overnight incubation maintained the same sensitization profile while increasing sensitivity. Reproducibility was demonstrated (R = 0.97), as was specificity using protein inhibition assays. Arthropods, fungi, and pollens showed the greatest discrimination for SEA. Conclusions: We have established that protein microarrays can be used for large-scale IgE mapping of allergens associated with the environment of horses. This technology provides a sound platform for specific diagnosis, management, and treatment of SEA.
© 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2019-08-20 PubMed ID: 31429513PubMed Central: PMC6766494DOI: 10.1111/jvim.15564Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on creating a comprehensive protein microarray platform for analyzing immunoglobulin E (IgE) allergies in horses exhibiting severe asthma or SEA. This method revealed new allergens and established an association between SEA and pollen sensitization in horses.

Research Objective and Methods

The primary goal of the study was to create a protein microarray platform that could profile IgE, which reacts to different environmental proteins in horses suffering from SEA. The researchers utilized protein extracts and purified proteins from diverse allergen families like pollen, bacteria, fungi, and arthropods found in the horses’ environment.

  • Six horses with SEA and six healthy Warmblood performance horses were used for the study.
  • The research team optimized and evaluated several parameters like printing, incubation, immunolabeling, biological fluid source, techniques for concentration, reproducibility, and specificity in the course of the research.

Results of the Research

The protein microarray method developed identified new allergens and linked SEA to pollen sensitization in horses.

  • The team confirmed the accuracy of a readily available mouse anti-horse IgE 3H10 source with immunolabeling methods.
  • Both serum and bronchoalveolar lavage fluid (BALF) gave the same specific IgE profile.
  • Amicon centrifugal filters were determined as the most effective method to concentrate BALF for IgE analysis.
  • An overnight incubation increased the sensitivity while keeping the sensitization profile the same.
  • Protein inhibition assays were used to demonstrate the method’s specificity, and reproducibility was also proven.
  • Arthropods, fungi, and pollens showed the most significant differentiation for SEA.

Conclusion

The study established that protein microarrays could effectively map large-scale IgE allergens associated with horses’ environment. This novel technology can provide a sturdy basis for the specific diagnosis, management, and treatment of SEA in horses.

Cite This Article

APA
White S, Moore-Colyer M, Marti E, Coüetil L, Hannant D, Richard EA, Alcocer M. (2019). Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma. J Vet Intern Med, 33(5), 2327-2335. https://doi.org/10.1111/jvim.15564

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 33
Issue: 5
Pages: 2327-2335

Researcher Affiliations

White, Samuel
  • School of Equine Management and Science, Royal Agricultural University, Gloucestershire, United Kingdom.
  • School of Biosciences, University of Nottingham, Loughborough, United Kingdom.
  • Animal and Equine Science, Nottingham Trent University, Nottinghamshire, United Kingdom.
Moore-Colyer, Meriel
  • School of Equine Management and Science, Royal Agricultural University, Gloucestershire, United Kingdom.
Marti, Eliane
  • Department of Clinical Research and Veterinary Public Health, University of Bern, Bern, Switzerland.
Coüetil, Laurent
  • Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.
Hannant, Duncan
  • School of Veterinary Medicine and Science, University of Nottingham, Loughborough, United Kingdom.
Richard, Eric A
  • LABÉO Frank Duncombe, Caen Cedex, France.
  • Normandie University, UniCaen, BIOTARGEN, Saint-Contest, France.
Alcocer, Marcos
  • School of Biosciences, University of Nottingham, Loughborough, United Kingdom.

MeSH Terms

  • Animals
  • Arthropods / immunology
  • Asthma / blood
  • Asthma / immunology
  • Asthma / veterinary
  • Bronchoalveolar Lavage Fluid / immunology
  • Case-Control Studies
  • Fungi / immunology
  • Horse Diseases / blood
  • Horse Diseases / immunology
  • Horses
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Mice
  • Pollen / immunology
  • Protein Array Analysis / methods
  • Protein Array Analysis / veterinary

Grant Funding

  • Fred And Marjorie Sainsbury Charitable Trust
  • HAYGAIN
  • The Royal Agricultural University

Conflict of Interest Statement

Authors declare no conflict of interest.

References

This article includes 54 references
  1. Hotchkiss JW, Reid SW, Christley RM. A survey of horse owners in Great Britain regarding horses in their care. Part 1: Horse demographic characteristics and management.. Equine Vet J 2007 Jul;39(4):294-300.
    pubmed: 17722719doi: 10.2746/042516407x177538google scholar: lookup
  2. Moran G, Folch H. Recurrent airway obstruction in horses—an allergic inflammation: a review.. Vet Med (Praha) 2011;56(1):1‐13.
  3. Gerber V, Swinburne JE, Blott SC, Nussbaumer P, Ramseyer A, Klukowska-Rötzler J, Dolf G, Marti E, Burger D, Leeb T. [Genetics of recurrent airway obstruction (RAO)].. Dtsch Tierarztl Wochenschr 2008 Jul;115(7):271-5.
    pubmed: 18672738
  4. Miskovic M, Couëtil LL, Thompson CA. Lung function and airway cytologic profiles in horses with recurrent airway obstruction maintained in low-dust environments.. J Vet Intern Med 2007 Sep-Oct;21(5):1060-6.
    pubmed: 17939565doi: 10.1892/0891-6640(2007)21[1060:lfaacp]2.0.co;2google scholar: lookup
  5. Pirie RS. Recurrent airway obstruction: a review.. Equine Vet J 2014 May;46(3):276-88.
    pubmed: 24164473doi: 10.1111/evj.12204google scholar: lookup
  6. Leclere M, Lavoie-Lamoureux A, Joubert P, Relave F, Setlakwe EL, Beauchamp G, Couture C, Martin JG, Lavoie JP. Corticosteroids and antigen avoidance decrease airway smooth muscle mass in an equine asthma model.. Am J Respir Cell Mol Biol 2012 Nov;47(5):589-96.
    pubmed: 22721832doi: 10.1165/rcmb.2011-0363ocgoogle scholar: lookup
  7. Bogacka E, Jahnz-Rózyk K. [Allergy to fungal antigens].. Pol Merkur Lekarski 2003 May;14(83):381-4.
    pubmed: 12939807
  8. Künzle F, Gerber V, Van Der Haegen A, Wampfler B, Straub R, Marti E. IgE-bearing cells in bronchoalveolar lavage fluid and allergen-specific IgE levels in sera from RAO-affected horses.. J Vet Med A Physiol Pathol Clin Med 2007 Feb;54(1):40-7.
    pubmed: 17359454doi: 10.1111/j.1439-0442.2007.00870.xgoogle scholar: lookup
  9. Morán G, Ojeda G, Diedrichs K, Ortloff A, Barria M, Folch H. Inhalation of Aspergillus fumigatus spores induces airway inflammation in mice in a similar manner as observed in recurrent airway obstruction in horses.. Arch Med Vet 2011;43(2):163‐171.
  10. Pirie RS, Dixon PM, McGorum BC. Endotoxin contamination contributes to the pulmonary inflammatory and functional response to Aspergillus fumigatus extract inhalation in heaves horses.. Clin Exp Allergy 2003 Sep;33(9):1289-96.
    pubmed: 12956737doi: 10.1046/j.1365-2745.2003.01651.xgoogle scholar: lookup
  11. Tahon L, Baselgia S, Gerber V, Doherr MG, Straub R, Robinson NE, Marti E. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009 Jan 15;127(1-2):85-93.
    pubmed: 19027178doi: 10.1016/j.vetimm.2008.09.021google scholar: lookup
  12. Jackson CA, Berney C, Jefcoat AM, Robinson NE. Environment and prednisone interactions in the treatment of recurrent airway obstruction (heaves).. Equine Vet J 2000 Sep;32(5):432-8.
    pubmed: 11037266doi: 10.2746/042516400777591165google scholar: lookup
  13. Cornelisse CJ, Robinson NE. Glucocorticoid therapy and the risk of equine laminitis.. Equine Vet Educ 2013;25(1):39‐46.
  14. Thomson JR, McPherson EA. Chronic obstructive pulmonary disease in the horse. 2: Therapy.. Equine Vet J 1983 Jul;15(3):207-10.
    pubmed: 6411459doi: 10.1111/j.2042-3306.1983.tb01766.xgoogle scholar: lookup
  15. Couëtil LL, Chilcoat CD, DeNicola DB, Clark SP, Glickman NW, Glickman LT. Randomized, controlled study of inhaled fluticasone propionate, oral administration of prednisone, and environmental management of horses with recurrent airway obstruction.. Am J Vet Res 2005 Oct;66(10):1665-74.
    pubmed: 16273895doi: 10.2460/ajvr.2005.66.1665google scholar: lookup
  16. Gerber H, Hockenjos P, Lazary S, Kings M, de Weck A. [Histamine release from equine leucocytes provoked by fungal allergens].. Dtsch Tierarztl Wochenschr 1982 Jul 6;89(7):267-70.
    pubmed: 6181953
  17. Dirscherl P, Grabner A, Buschmann H. Responsiveness of basophil granulocytes of horses suffering from chronic obstructive pulmonary disease to various allergens.. Vet Immunol Immunopathol 1993 Oct;38(3-4):217-27.
    pubmed: 7507274doi: 10.1016/0165-2427(93)90082-fgoogle scholar: lookup
  18. Hare JE, Viel L, Conlon PD, Marshall JS. In vitro allergen-induced degranulation of pulmonary mast cells from horses with recurrent airway obstruction (heaves).. Am J Vet Res 1999 Jul;60(7):841-7.
    pubmed: 10407477
  19. McGorum BC, Dixon PM, Halliwell RE. Quantification of histamine in plasma and pulmonary fluids from horses with chronic obstructive pulmonary disease, before and after 'natural (hay and straw) challenges'.. Vet Immunol Immunopathol 1993 Apr;36(3):223-37.
    pubmed: 8506613doi: 10.1016/0165-2427(93)90021-ugoogle scholar: lookup
  20. McPherson EA, Lawson GH, Murphy JR, Nicholson JM, Breeze RG, Pirie HM. Chronic obstructive pulmonary disease (COPD) in horses: aetiological studies: responses to intradermal and inhalation antigenic challenge.. Equine Vet J 1979 Jul;11(3):159-66.
    pubmed: 385306doi: 10.1111/j.2042-3306.1979.tb01330.xgoogle scholar: lookup
  21. Halliwell RE, McGorum BC, Irving P, Dixon PM. Local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.. Vet Immunol Immunopathol 1993 Oct;38(3-4):201-15.
    pubmed: 8291200doi: 10.1016/0165-2427(93)90081-egoogle scholar: lookup
  22. Marti E, Wang X, Jambari NN, Rhyner C, Olzhausen J, Pérez-Barea JJ, Figueredo GP, Alcocer MJ. Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.. Vet Immunol Immunopathol 2015 Oct 15;167(3-4):171-7.
    pubmed: 26163936doi: 10.1016/j.vetimm.2015.06.013google scholar: lookup
  23. Eder C, Crameri R, Mayer C, Eicher R, Straub R, Gerber H, Lazary S, Marti E. Allergen-specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.. Vet Immunol Immunopathol 2000 Mar 15;73(3-4):241-53.
    pubmed: 10713338doi: 10.1016/s0165-2427(00)00154-9google scholar: lookup
  24. Niedzwiedz A, Jaworski Z, Kubiak K. Serum concentrations of allergen-specific IgE in horses with equine recurrent airway obstruction and healthy controls assessed by ELISA.. Vet Clin Pathol 2015 Sep;44(3):391-6.
    pubmed: 26175133doi: 10.1111/vcp.12274google scholar: lookup
  25. Schmallenbach KH, Rahman I, Sasse HH, Dixon PM, Halliwell RE, McGorum BC, Crameri R, Miller HR. Studies on pulmonary and systemic Aspergillus fumigatus-specific IgE and IgG antibodies in horses affected with chronic obstructive pulmonary disease (COPD).. Vet Immunol Immunopathol 1998 Dec 11;66(3-4):245-56.
    pubmed: 9880102doi: 10.1016/s0165-2427(98)00202-5google scholar: lookup
  26. Crameri R, Garbani M, Rhyner C, Huitema C. Fungi: the neglected allergenic sources.. Allergy 2014 Feb;69(2):176-85.
    pubmed: 24286281doi: 10.1111/all.12325google scholar: lookup
  27. Fall BI, Niessner R. Detection of known allergen-specific IgE antibodies by immunological methods.. Methods Mol Biol 2009;509:107-22.
    doi: 10.1007/978-1-59745-372-1_7pubmed: 19212717google scholar: lookup
  28. Jambari NN, Wang X, Alcocer M. Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis.. Methods Mol Biol 2017;1592:129-137.
    pubmed: 28315216doi: 10.1007/978-1-4939-6925-8_10google scholar: lookup
  29. Jahn-Schmid B, Harwanegg C, Hiller R, Bohle B, Ebner C, Scheiner O, Mueller MW. Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin E.. Clin Exp Allergy 2003 Oct;33(10):1443-9.
    pubmed: 14519153doi: 10.1046/j.1365-2222.2003.01784.xgoogle scholar: lookup
  30. Renault NK, Gaddipati SR, Wulfert F, Falcone FH, Mirotti L, Tighe PJ, Wright V, Alcocer MJ. Multiple protein extract microarray for profiling human food-specific immunoglobulins A, M, G and E.. J Immunol Methods 2011 Feb 1;364(1-2):21-32.
    pubmed: 20974146doi: 10.1016/j.jim.2010.10.004google scholar: lookup
  31. Jeon H, Jung JH, Kim Y, Kwon Y, Kim ST. Allergen Microarrays for In Vitro Diagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure.. Ann Lab Med 2018 Jul;38(4):338-347.
    pmc: PMC5895863pubmed: 29611384doi: 10.3343/alm.2018.38.4.338google scholar: lookup
  32. Ivester KM, Couëtil LL, Moore GE, Zimmerman NJ, Raskin RE. Environmental exposures and airway inflammation in young thoroughbred horses.. J Vet Intern Med 2014 May-Jun;28(3):918-24.
    pmc: PMC4895475pubmed: 24773603doi: 10.1111/jvim.12333google scholar: lookup
  33. Eder C, Curik I, Brem G, Crameri R, Bodo I, Habe F, Lazary S, Sölkner J, Marti E. Influence of environmental and genetic factors on allergen-specific immunoglobulin-E levels in sera from Lipizzan horses.. Equine Vet J 2001 Nov;33(7):714-20.
    pubmed: 11770995doi: 10.2746/042516401776249264google scholar: lookup
  34. Lin J, Bardina L, Shreffler WG, Andreae DA, Ge Y, Wang J, Bruni FM, Fu Z, Han Y, Sampson HA. Development of a novel peptide microarray for large-scale epitope mapping of food allergens.. J Allergy Clin Immunol 2009 Aug;124(2):315-22, 322.e1-3.
    pmc: PMC2757036pubmed: 19577281doi: 10.1016/j.jaci.2009.05.024google scholar: lookup
  35. Froidure A, Mouthuy J, Durham SR, Chanez P, Sibille Y, Pilette C. Asthma phenotypes and IgE responses.. Eur Respir J 2016 Jan;47(1):304-19.
    pubmed: 26677936doi: 10.1183/13993003.01824-2014google scholar: lookup
  36. Wilson AD, Harwood L, Torsteinsdottir S, Marti E. Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandic and non-Icelandic horses with insect bite dermal hypersensitivity.. Vet Immunol Immunopathol 2006 Aug 15;112(3-4):156-70.
    pubmed: 16574245doi: 10.1016/j.vetimm.2006.02.003google scholar: lookup
  37. Smoldovskaya O, Feyzkhanova G, Arefieva A, Voloshin S, Ivashkina O, Reznikov Y, Rubina A. Allergen extracts and recombinant proteins: comparison of efficiency of in vitro allergy diagnostics using multiplex assay on a biological microchip.. Allergy Asthma Clin Immunol 2016;12:9.
    pmc: PMC4789275pubmed: 26973700doi: 10.1186/s13223-016-0117-1google scholar: lookup
  38. Dodig S, Čepelak I. The potential of component-resolved diagnosis in laboratory diagnostics of allergy.. Biochem Med (Zagreb) 2018 Jun 15;28(2):020501.
    pmc: PMC5898957pubmed: 29666553doi: 10.11613/bm.2018.020501google scholar: lookup
  39. Matricardi P, Stringari G, Caffarelli C, Asero R, Dondi A, Tripodi S. The impact of component resolved diagnosis on allergen‐specific immunotherapy prescription in children with pollen‐related allergic rhinitis.. J Allergy Clin Immunol 2014;133(2):AB45.
  40. Wilson DR, Merrett TG, Varga EM, Smurthwaite L, Gould HJ, Kemp M, Hooper J, Till SJ, Durham SR. Increases in allergen-specific IgE in BAL after segmental allergen challenge in atopic asthmatics.. Am J Respir Crit Care Med 2002 Jan 1;165(1):22-6.
    pubmed: 11779725doi: 10.1164/ajrccm.165.1.2010112google scholar: lookup
  41. Wilkie BN, Markham RJ. Sequential titration of bovine lung and serum antibodies after parenteral or pulmonary inoculation with Pasteurella haemolytica.. Am J Vet Res 1979 Dec;40(12):1690-3.
    pubmed: 525887
  42. Peebles RS Jr, Hamilton RG, Lichtenstein LM, Schlosberg M, Liu MC, Proud D, Togias A. Antigen-specific IgE and IgA antibodies in bronchoalveolar lavage fluid are associated with stronger antigen-induced late phase reactions.. Clin Exp Allergy 2001 Feb;31(2):239-48.
    pubmed: 11251625doi: 10.1046/j.1365-2222.2001.01048.xgoogle scholar: lookup
  43. Koh YY, Park Y, Lee HJ, Kim CK. Levels of interleukin-2, interferon-gamma, and interleukin-4 in bronchoalveolar lavage fluid from patients with Mycoplasma pneumonia: implication of tendency toward increased immunoglobulin E production.. Pediatrics 2001 Mar;107(3):E39.
    pubmed: 11230620doi: 10.1542/peds.107.3.e39google scholar: lookup
  44. Marti E, Peveri P, Griot-Wenk M, Muntwyler J, Crameri R, Schaller J, Gerber H, Lazary S. Chicken antibodies to a recombinant fragment of the equine immunoglobulin epsilon heavy-chain recognising native horse IgE.. Vet Immunol Immunopathol 1997 Nov;59(3-4):253-70.
    pubmed: 9477476doi: 10.1016/s0165-2427(97)00096-2google scholar: lookup
  45. Bartlett JA, Albertolle ME, Wohlford-Lenane C, Pezzulo AA, Zabner J, Niles RK, Fisher SJ, McCray PB Jr, Williams KE. Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs.. Am J Physiol Lung Cell Mol Physiol 2013 Aug 1;305(3):L256-66.
    pmc: PMC3743012pubmed: 23709621doi: 10.1152/ajplung.00056.2013google scholar: lookup
  46. Peebles RS Jr, Liu MC, Adkinson NF Jr, Lichtenstein LM, Hamilton RG. Ragweed-specific antibodies in bronchoalveolar lavage fluids and serum before and after segmental lung challenge: IgE and IgA associated with eosinophil degranulation.. J Allergy Clin Immunol 1998 Feb;101(2 Pt 1):265-73.
    pubmed: 9500761doi: 10.1016/s0091-6749(98)70392-6google scholar: lookup
  47. Lamkhioued B, Renzi PM, Abi-Younes S, Garcia-Zepada EA, Allakhverdi Z, Ghaffar O, Rothenberg MD, Luster AD, Hamid Q. Increased expression of eotaxin in bronchoalveolar lavage and airways of asthmatics contributes to the chemotaxis of eosinophils to the site of inflammation.. J Immunol 1997 Nov 1;159(9):4593-601.
    pubmed: 9379061
  48. Richard EA, Depecker M, Defontis M, Leleu C, Fortier G, Pitel PH, Couroucé-Malblanc A. Cytokine concentrations in bronchoalveolar lavage fluid from horses with neutrophilic inflammatory airway disease.. J Vet Intern Med 2014 Nov-Dec;28(6):1838-44.
    pmc: PMC4895612pubmed: 25269933doi: 10.1111/jvim.12464google scholar: lookup
  49. Foster MW, Thompson JW, Que LG, Yang IV, Schwartz DA, Moseley MA, Marshall HE. Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.. J Proteome Res 2013 May 3;12(5):2194-205.
    pmc: PMC3822014pubmed: 23550723doi: 10.1021/pr400066ggoogle scholar: lookup
  50. Kneidinger N, Warszawska J, Schenk P, Fuhrmann V, Bojic A, Hirschl A, Herkner H, Madl C, Makristathis A. Storage of bronchoalveolar lavage fluid and accuracy of microbiologic diagnostics in the ICU: a prospective observational study.. Crit Care 2013 Jul 11;17(4):R135.
    pmc: PMC4057171pubmed: 23844796doi: 10.1186/cc12814google scholar: lookup
  51. Huang X, Tsilochristou O, Perna S, Hofmaier S, Cappella A, Bauer CP, Hoffman U, Forster J, Zepp F, Schuster A, D'Amelio R, Wahn U, Keil T, Lau S, Matricardi PM. Evolution of the IgE and IgG repertoire to a comprehensive array of allergen molecules in the first decade of life.. Allergy 2018 Feb;73(2):421-430.
    pubmed: 28791748doi: 10.1111/all.13269google scholar: lookup
  52. Rodríguez-Capote K, Schnabl KL, Maries OR, Janzen P, Higgins TN. Stability of specific IgE antibodies to common food and inhalant allergens.. Clin Biochem 2016 Dec;49(18):1387-1389.
    pubmed: 26994557doi: 10.1016/j.clinbiochem.2016.03.003google scholar: lookup
  53. Sridhara S, Singh BP, Kumar L, Verma J, Gaur SN, Gangal SV. Antigenic and allergenic relationships among airborne grass pollens in India.. Ann Allergy Asthma Immunol 1995 Jul;75(1):73-9.
    pubmed: 7621065
  54. Vojdani A. Cross-reactivity of Aspergillus, Penicillium, and Stachybotrys antigens using affinity-purified antibodies and immunoassay.. Arch Environ Health 2004 May;59(5):256-65.
    pubmed: 16201672doi: 10.3200/aeoh.59.5.256-265google scholar: lookup

Citations

This article has been cited 8 times.
  1. Wyler M, Sage SE, Marti E, White S, Gerber V. Protein microarray allergen profiling in bronchoalveolar lavage fluid and serum of horses with asthma. J Vet Intern Med 2023 Jan;37(1):328-337.
    doi: 10.1111/jvim.16600pubmed: 36479920google scholar: lookup
  2. Di Pietro R, Dubuc V, Manguin E, Giroux-Lafond R, Bédard C, Boivin R, Lavoie JP, Vesper SJ, Leclere M. Characterization of fungal exposure and dectin-1 expression in healthy horses and horses with severe asthma. Am J Vet Res 2022 May 8;83(6).
    doi: 10.2460/ajvr.21.09.0143pubmed: 35524958google scholar: lookup
  3. Birras J, White SJ, Jonsdottir S, Novotny EN, Ziegler A, Wilson AD, Frey R, Torsteinsdottir S, Alcocer M, Marti E. First clinical expression of equine insect bite hypersensitivity is associated with co-sensitization to multiple Culicoides allergens. PLoS One 2021;16(11):e0257819.
    doi: 10.1371/journal.pone.0257819pubmed: 34780496google scholar: lookup
  4. White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep 2019 Oct 23;9(1):15170.
    doi: 10.1038/s41598-019-51820-7pubmed: 31645629google scholar: lookup
  5. Dély S, Gerber V, Peters LM, Sage SE. Association between equine asthma and fungal elements in the tracheal wash: An environment-matched case-control study. PLoS One 2024;19(9):e0309835.
    doi: 10.1371/journal.pone.0309835pubmed: 39240830google scholar: lookup
  6. Wjst VF, Lübke S, Wagner B, Rhyner C, Jentsch MC, Arnold C, Lohmann KL, Schnabel CL. Aspergillus fumigatus antigen-reactive Th17 cells are enriched in bronchoalveolar lavage fluid in severe equine asthma. Front Immunol 2024;15:1367971.
    doi: 10.3389/fimmu.2024.1367971pubmed: 39229267google scholar: lookup
  7. Schnabel CL, Jentsch MC, Lübke S, Kaiser-Thom S, Gerber V, Vrtala S, Huang HJ, Rhyner C, Wagner B, Hoffmann R, Volke D. Immunoproteomics reveal increased serum IgG3/5 binding to Dermatophagoides and yeast protein antigens in severe equine asthma in a preliminary study. Front Immunol 2023;14:1293684.
    doi: 10.3389/fimmu.2023.1293684pubmed: 38162673google scholar: lookup
  8. White SJ, Couetil L, Richard EA, Marti E, Wilson PB. Microarray molecular mapping of horses with severe asthma. J Vet Intern Med 2024 Jan-Feb;38(1):477-484.
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