Development of a single multi-locus sequence typing scheme for Taylorella equigenitalis and Taylorella asinigenitalis.
Abstract: We describe here the development of a multilocus sequence typing (MLST) scheme for Taylorella equigenitalis, the causative agent of contagious equine metritis (CEM), and Taylorella asinigenitalis, a nonpathogenic bacterium. MLST was performed on a set of 163 strains collected in several countries over 35 years (1977-2012). The MLST data were analyzed using START2, MEGA 5.05 and eBURST, and can be accessed at http://pubmlst.org/taylorella/. Our results revealed a clonal population with 39 sequence types (ST) and no common ST between the two Taylorella species. The eBURST analysis grouped the 27 T. equigenitalis STs into four clonal complexes (CC1-4) and five unlinked STs. The 12 T. asinigenitalis STs were grouped into three clonal complexes (CC5-7) and five unlinked STs, among which CC1 (68.1% of the 113 T. equigenitalis) and CC5 (58.0% of the 50 T. asinigenitalis) were dominants. The CC1, still in circulation in France, contains isolates from the first CEM outbreaks that simultaneously emerged in several countries in the late 1970s. The emergence in different countries (e.g. France, Japan, and United Arab Emirates) of STs without any genetic relationship to CC1 suggests the existence of a natural worldwide reservoir that remains to be identified. T. asinigenitalis appears to behave same way since the American, Swedish and French isolates have unrelated STs. This first Taylorella sp. MLST is a powerful tool for further epidemiological investigations and population biology studies of the Taylorella genus.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-09-24 PubMed ID: 24139720DOI: 10.1016/j.vetmic.2013.09.016Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article outlines the development of a new sequence typing strategy for identifying and studying bacteria that cause disease in horses. The multi-locus sequence typing (MLST) scheme was developed specifically for Taylorella equigenitalis and Taylorella asinigenitalis, bacterial species that impact equine health.
Overview of Multi-locus Sequence Typing (MLST)
- MLST is a technique used in molecular biology for the genetic identification and study of bacteria.
- It operates by sequencing internal fragments of multiple housekeeping genes and using this information to characterize the bacterial strain.
Application of MLST to Taylorella equigenitalis and Taylorella asinigenitalis
- These two bacterial species were chosen for development of an MLST scheme because of their relevance in equine health.
- Taylorella equigenitalis is the bacteria responsible for contagious equine metritis (CEM), while Taylorella asinigenitalis is a non-pathogenic bacterium.
- In this research, 163 strains of these bacteria, collected from various countries over a 35-year period, were analyzed using MLST.
Results from the MLST Analysis
- The MLST data analysis revealed 39 sequence types, with no common type between the two bacterial species.
- The analysis grouped certain sequence types into clonal complexes, which represent groups of strains that are genetically close enough to be considered derived from a common ancestor.
- The data also suggested the existence of a natural worldwide reservoir, as the study found sequence types emerging in different countries without any genetic relationship to the dominant clonal complex.
Implications of the Study
- The MLST scheme developed in this study provides a new tool for further epidemiological studies and population biology analyses of the Taylorella genus.
- Furthermore, the identification of dominant clonal complexes, as well as unlinked sequence types, can provide insights into the evolution, spread, and diversity of these bacterial species.
Cite This Article
APA
Duquesne F, Hébert L, Breuil MF, Matsuda M, Laugier C, Petry S.
(2013).
Development of a single multi-locus sequence typing scheme for Taylorella equigenitalis and Taylorella asinigenitalis.
Vet Microbiol, 167(3-4), 609-618.
https://doi.org/10.1016/j.vetmic.2013.09.016 Publication
Researcher Affiliations
- ANSES, Dozulé Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, 14430 Dozulé, France. Electronic address: fabien.duquesne@anses.fr.
MeSH Terms
- Animals
- Bacterial Typing Techniques / methods
- Female
- Genes, Bacterial / genetics
- Genetic Variation
- Gram-Negative Bacterial Infections / microbiology
- Gram-Negative Bacterial Infections / veterinary
- Horse Diseases / microbiology
- Horses / genetics
- Male
- Molecular Typing
- Multilocus Sequence Typing / veterinary
- Phylogeny
- Taylorella / classification
- Taylorella / genetics
- Taylorella / isolation & purification
- Taylorella equigenitalis / classification
- Taylorella equigenitalis / genetics
- Taylorella equigenitalis / isolation & purification
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