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Home / Equine Research Bank / Animals (Basel) / Development of a Web App to Convert Blood Insulin Concentrat...
Animals : an open access journal from MDPI2023; 13(17); 2704; doi: 10.3390/ani13172704

Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses.

Abstract: The measurement of the blood insulin concentration, and comparison to cut-offs, is essential in diagnosing insulin dysregulation, a common equine endocrinopathy. However, different insulin assays provide disparate results. We aimed to ease comparison between assays by compiling original and published data into a web app to convert insulin measurements from one assay to another. Data were available for ADVIA Centaur insulin chemiluminescent immunoassay (CLIA), Beckman Coulter insulin radioimmunoassay (RIA), Immulite 1000 CLIA, Immulite 2000 CLIA, Immulite 2000 XPi CLIA, Mercodia equine insulin enzyme-linked immunosorbent assay (ELISA), and Millipore porcine insulin RIA. Linear models were fitted for 13 assay pairs using non-decreasing splines, and integrated into this app. Assay comparisons including data from several studies showed a lower performance. This indicates technical variation between laboratories, which has not been described before, but is relevant when diagnostic measurements and cut-offs are provided by different laboratories. Nevertheless, the models' overall high performance (median = 0.94; range 0.57-1.00) supports their use to interpret results from diagnostic insulin measurements when the reference assay is unavailable, and to compare values obtained from different assays.
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Publication Date: 2023-08-24 PubMed ID: 37684968PubMed Central: PMC10487020DOI: 10.3390/ani13172704Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research revolves around the creation of a web application designed to convert various insulin measurement metrics from different immunoassays, specifically for horse blood insulin concentrations. The web application is a solution to resolve discrepancies in blood insulin measurements from different tests and to streamline diagnosis of insulin dysregulation in horses.

Background and Aim

  • The study commences with the importance of accurately measuring blood insulin concentration in horses for diagnosing insulin dysregulation, a recognized equine endocrine disorder.
  • However, it notes that different types of insulin assays often provide varied results, posing a challenge for accurate diagnosis.
  • In response to this issue, the research aims to develop a web application that can convert insulin measurements from one type of assay to another, effectively allowing for straightforward comparisons.

Methodology

  • The researchers compiled original and previously published data relevant to several commonly used assays. This included the ADVIA Centaur insulin chemiluminescent immunoassay (CLIA), Beckman Coulter insulin radioimmunoassay (RIA), Immulite 1000 CLIA, Immulite 2000 CLIA, Immulite 2000 XPi CLIA, Mercodia equine insulin enzyme-linked immunosorbent assay (ELISA), and Millipore porcine insulin RIA.
  • The data collected was then used to create linear models for 13 different assay pairings using non-decreasing splines. These models were then integrated into the web application.

Results and Findings

  • The study found some variations in performance when assay data from different studies were compared. This highlighted previously unexplained technical variations between laboratories, which could significantly impact diagnosis if measurements and cut-offs provided by these different laboratories were to be compared.
  • Despite such variations, the models’ largely high performance rates (median = 0.94; range 0.57-1.00) suggest that they may be employed to interpret results from different insulin measurement tests, especially when the reference assay is not available.
  • The models in the application can also facilitate the comparison of blood insulin concentration levels obtained from different assays. Thus, the application can contribute to more accurate and consistent diagnoses of insulin dysregulation in horses.

Cite This Article

APA
Delarocque J, Feige K, Carslake HB, Durham AE, Fey K, Warnken T. (2023). Development of a Web App to Convert Blood Insulin Concentrations among Various Immunoassays Used in Horses. Animals (Basel), 13(17), 2704. https://doi.org/10.3390/ani13172704

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 17
PII: 2704

Researcher Affiliations

Delarocque, Julien
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.
Feige, Karsten
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.
Carslake, Harry B
  • Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Leahurst, Neston CH64 7TE, Cheshire, UK.
Durham, Andy E
  • The Liphook Equine Hospital, Forest Mere, Liphook GU30 7JG, Hampshire, UK.
Fey, Kerstin
  • Equine Clinic, Internal Medicine, Faculty of Veterinary Medicine, Justus-Liebig-University of Giessen, Frankfurter Str. 126, 35392 Giessen, Germany.
Warnken, Tobias
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest.

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