Development of an equine herpesvirus in two cell culture systems: light and electron microscopy.
Abstract: Development of equine herpesvirus strain 82A was studied in cells from primary horse kidney (HOK) cultures and an equine dermis (ED) cell strain. HOK and ED cells are equally susceptible to the 82A virus infection and yield about the same amount of infectious virus. Intranuclear inclusions were present in both cell systems, but a ring-shaped syncytial formation was observed only in infected ED cells. Ultrastructural studies revealed the presence of dense granules 30 nm in diameter and characteristic star-like clusters of granules in the infected HOK cells, but these granules were rarely seen in the infected ED cells. Viral nucleocapsids were associated with homogenous nuclear matrices, with moderate electron density in both cell systems. Viral nucleocapsids acquired envelopes by budding into the nuclear vacuoles in both HOK and ED cells. Budding from inner nuclear membranes into perinuclear cisterna or into cytoplasmic vacuoles also was observed frequently in HOK cells but was not seen often in infected ED cells. Multiple, membrane-bound intranuclear inclusions of fibrillar material which may be associated with virus envelopes were seen only in infected ED cells. Enveloped virus particles seen in nuclear vacuoles or perinuclear cisterna were more regular in shape and had a 130-nm diameter, whereas the enveloped virus particles seen in the cytoplasm and extracellular space were more irregular in shape and had a 130- to 160-nm diameter.
Publication Date: 1972-11-01 PubMed ID: 4673984PubMed Central: PMC422622DOI: 10.1128/iai.6.5.865-876.1972Google Scholar: Lookup
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- Journal Article
Summary
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This study examined how the equine herpesvirus strain 82A developed in two different cell cultures, primary horse kidney (HOK) and equine dermis (ED), under light and electron microscope examination. The study found similarities and differences in virus development across these cell cultures and identified unique characteristics of the particles of the virus in each.
Objective of Research
- The study aimed to understand the development of the equine herpesvirus strain 82A in primary horse kidney (HOK) cultures and equine dermis (ED) cell culture systems and observe any differences in the development.
Virus Infection in HOK and ED Cells
- The same virus strain was found to infect both HOK and ED cells equally, producing almost similar amounts of the virus in each cell type.
- During the infection, intranuclear inclusions, collections of viral components within the cell nuclei, were observed in both kinds of cells.
Differences in Infection Pattern
- A ring-shaped syncytial formation, a structure formed by the fusion of multiple cells, was only found in the infected ED cells.
- Granules and star-like clusters of granules were observed in infected HOK cells, but these were uncommon in ED cells. These granules could play a role in the development or transportation of the virus within the cell.
Intranuclear Inclusions and Virus Envelopes
- Within the infected cell nuclei, viral nucleocapsids (the protective covering of the genetic material of the virus) were found within homogeneous nuclear matrices in both HOK and ED cells.
- Viral envelopes, the outer layer of the virus particle, were seen to form by budding into the nuclear vacuoles (empty spaces within the cell nucleus) in both HOK and ED cells.
- In HOK cells, the envelope formation by budding from inner nuclear membranes into perinuclear cisterna or into cytoplasmic vacuoles was observed more frequently than in ED cells.
Findings in Infected ED Cells and Nature of Virus Particles
- In infected ED cells, multiple, membrane-bound inclusions of virus-associated fibrillar material were observed.
- The virus particles floating in nuclear vacuoles or perinuclear cisterna generally had a regular shape with a size of approximately 130nm.
- The virus particles observed in the cytoplasm and extracellular space were irregular in shape and larger in size, ranging from 130nm to 160nm.
Cite This Article
APA
Fong CK, Hsiung GD.
(1972).
Development of an equine herpesvirus in two cell culture systems: light and electron microscopy.
Infect Immun, 6(5), 865-876.
https://doi.org/10.1128/iai.6.5.865-876.1972 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Herpesviridae
- Horses
- Inclusion Bodies, Viral
- Kidney / microbiology
- Microscopy, Electron
- Skin / microbiology
References
This article includes 17 references
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Citations
This article has been cited 3 times.- Jönsson L, Beck-Friis J, Renström LH, Nikkilä T, Thebo P, Sundquist B. Equine herpes virus 1 (EHV-1) in liver, spleen, and lung as demonstrated by immunohistology and electron microscopy.. Acta Vet Scand 1989;30(2):141-6.
- Valícek L, Smíd B. Envelopment and the envelopes of infectious bovine rhinotracheitis virus in ultrathin sections.. Arch Virol 1976;51(1-2):131-40.
- Fong CK, Gross PA, Hsiung GD, Swack NS. Use of electron microscopy for detection of viral and other microbial contaminants in bovine sera.. J Clin Microbiol 1975 Feb;1(2):219-24.
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