Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus.
Abstract: An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) was developed for the detection of primary infection of vesicular stomatitis virus (VSV) in equine and swine sera. The test was based on the use of biotinylated sheep antibodies against equine or swine IgM molecules bound to a streptavidin-coated ELISA plate. The captured IgM antibodies were detected by application of antigens prepared from the New Jersey and the Indiana VSV serotypes (VSV-NJ and VSV-IN, respectively) and mouse polyclonal antibodies against VSV-NJ and VSV-IN. The MC-ELISA was compared to a competitive ELISA (C-ELISA) and the standard microtiter serum neutralization (MTSN) assay by testing serum samples from horses and pigs experimentally infected with VSV-NJ or VSV-IN. The MC-ELISA detected specific homologous IgM antibodies from equine and swine sera as early as 5 and 4 days postinfection (DPI), respectively, and as late as 35 DPI. The MTSN test also detected antibodies as early as 5 DPI and as late as 160 DPI. In a similar fashion, the C-ELISA detected antibodies from 6 to 7 DPI and as late as 160 DPI. These results demonstrated that the MC-ELISA is a useful test for serodiagnosis of primary VSV infection in horses and pigs.
Publication Date: 2001-05-01 PubMed ID: 11329443PubMed Central: PMC96086DOI: 10.1128/CDLI.8.3.475-481.2001Google Scholar: Lookup
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- Journal Article
Summary
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The study developed a lab-based technique called immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) to effectively track primary infections of vesicular stomatitis virus (VSV) in horses and pigs. The developed test demonstrated effectiveness over a broad time range of infection, indicating its utility in diagnosing primary VSV infection in these animals.
Methodology and procedure
- The study focused on creating an IgM capture ELISA (MC-ELISA) designed to pick up primary infections of vesicular stomatitis virus, a contagious disease among horses and pigs.
- The test was built using biotinylated sheep antibodies against horse or pig IgM molecules. These were bound to a plate covered in streptavidin, a protein that binds to biotin, a vitamin involved in converting food into energy.
- Detection of the captured IgM antibodies was made possible through the application of antigen solutions prepared from two serotypes of VSV – the New Jersey and Indiana variants – and mouse polyclonal antibodies targeted against these two variants.
Comparative Analysis
- Researcher conducted comparative analyses between MC-ELISA, competitive ELISA (C-ELISA), and the standard microtiter serum neutralization (MTSN) assay—traditional laboratory techniques used to detect specific antibodies or antigens.
- They tested serum samples extracted from horses and pigs who were experimentally infected with the New Jersey or Indiana variants of VSV.
- MC-ELISA was able to detect specific homologous IgM antibodies as early as 5 days post infection (DPI) in horses and 4 DPI in pigs and lasted as long as 35 DPI.
- In comparison, the MTSN test detected antibodies as early as 5 DPI and lasted up to 160 DPI. Similarly, C-ELISA picked up antibodies approximately 6 to 7 DPI and continued to detect them for up to 160 DPI.
Conclusion
- The outcome of the study reveals that the newly developed MC-ELISA proves to be an effective tool for the serodiagnosis of vesicular stomatitis virus infection in horses and pigs.
- This suggests that this test is a valuable tool for use in tracking the course and timing of primary infections in these animals.
Cite This Article
APA
Zhou EM, Riva J, Clavijo A.
(2001).
Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus.
Clin Diagn Lab Immunol, 8(3), 475-481.
https://doi.org/10.1128/CDLI.8.3.475-481.2001 Publication
Researcher Affiliations
- National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada. ezhou@iastate.edu
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Antibodies, Viral / immunology
- Enzyme-Linked Immunosorbent Assay / methods
- Horses
- Immunoglobulin M / analysis
- Immunoglobulin M / immunology
- Rhabdoviridae Infections / immunology
- Rhabdoviridae Infections / virology
- Swine
- Vesicular stomatitis Indiana virus / immunology
- Vesiculovirus
References
This article includes 19 references
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Citations
This article has been cited 2 times.- Lee HS, Heo EJ, Jeoung HY, Ko HR, Kweon CH, Youn HJ, Ko YJ. Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey. Clin Vaccine Immunol 2009 May;16(5):667-71.
- Burnouf T, Griffiths E, Padilla A, Seddik S, Stephano MA, Gutiérrez JM. Assessment of the viral safety of antivenoms fractionated from equine plasma. Biologicals 2004 Sep;32(3):115-28.
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