FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Clinical and diagnostic laboratory immunology2001; 8(3); 475-481; doi: 10.1128/CDLI.8.3.475-481.2001

Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus.

Abstract: An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) was developed for the detection of primary infection of vesicular stomatitis virus (VSV) in equine and swine sera. The test was based on the use of biotinylated sheep antibodies against equine or swine IgM molecules bound to a streptavidin-coated ELISA plate. The captured IgM antibodies were detected by application of antigens prepared from the New Jersey and the Indiana VSV serotypes (VSV-NJ and VSV-IN, respectively) and mouse polyclonal antibodies against VSV-NJ and VSV-IN. The MC-ELISA was compared to a competitive ELISA (C-ELISA) and the standard microtiter serum neutralization (MTSN) assay by testing serum samples from horses and pigs experimentally infected with VSV-NJ or VSV-IN. The MC-ELISA detected specific homologous IgM antibodies from equine and swine sera as early as 5 and 4 days postinfection (DPI), respectively, and as late as 35 DPI. The MTSN test also detected antibodies as early as 5 DPI and as late as 160 DPI. In a similar fashion, the C-ELISA detected antibodies from 6 to 7 DPI and as late as 160 DPI. These results demonstrated that the MC-ELISA is a useful test for serodiagnosis of primary VSV infection in horses and pigs.
Get Full Text
Publication Date: 2001-05-01 PubMed ID: 11329443PubMed Central: PMC96086DOI: 10.1128/CDLI.8.3.475-481.2001Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study developed a lab-based technique called immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) to effectively track primary infections of vesicular stomatitis virus (VSV) in horses and pigs. The developed test demonstrated effectiveness over a broad time range of infection, indicating its utility in diagnosing primary VSV infection in these animals.

Methodology and procedure

  • The study focused on creating an IgM capture ELISA (MC-ELISA) designed to pick up primary infections of vesicular stomatitis virus, a contagious disease among horses and pigs.
  • The test was built using biotinylated sheep antibodies against horse or pig IgM molecules. These were bound to a plate covered in streptavidin, a protein that binds to biotin, a vitamin involved in converting food into energy.
  • Detection of the captured IgM antibodies was made possible through the application of antigen solutions prepared from two serotypes of VSV – the New Jersey and Indiana variants – and mouse polyclonal antibodies targeted against these two variants.

Comparative Analysis

  • Researcher conducted comparative analyses between MC-ELISA, competitive ELISA (C-ELISA), and the standard microtiter serum neutralization (MTSN) assay—traditional laboratory techniques used to detect specific antibodies or antigens.
  • They tested serum samples extracted from horses and pigs who were experimentally infected with the New Jersey or Indiana variants of VSV.
  • MC-ELISA was able to detect specific homologous IgM antibodies as early as 5 days post infection (DPI) in horses and 4 DPI in pigs and lasted as long as 35 DPI.
  • In comparison, the MTSN test detected antibodies as early as 5 DPI and lasted up to 160 DPI. Similarly, C-ELISA picked up antibodies approximately 6 to 7 DPI and continued to detect them for up to 160 DPI.

Conclusion

  • The outcome of the study reveals that the newly developed MC-ELISA proves to be an effective tool for the serodiagnosis of vesicular stomatitis virus infection in horses and pigs.
  • This suggests that this test is a valuable tool for use in tracking the course and timing of primary infections in these animals.

Cite This Article

APA
Zhou EM, Riva J, Clavijo A. (2001). Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus. Clin Diagn Lab Immunol, 8(3), 475-481. https://doi.org/10.1128/CDLI.8.3.475-481.2001

Publication

Clinical and diagnostic laboratory immunology
ISSN: 1071-412X
NlmUniqueID: 9421292
Country: United States
Language: English
Volume: 8
Issue: 3
Pages: 475-481

Researcher Affiliations

Zhou, E M
  • National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada. ezhou@iastate.edu
Riva, J
    Clavijo, A

      MeSH Terms

      • Animals
      • Antibodies, Viral / analysis
      • Antibodies, Viral / immunology
      • Enzyme-Linked Immunosorbent Assay / methods
      • Horses
      • Immunoglobulin M / analysis
      • Immunoglobulin M / immunology
      • Rhabdoviridae Infections / immunology
      • Rhabdoviridae Infections / virology
      • Swine
      • Vesicular stomatitis Indiana virus / immunology
      • Vesiculovirus

      References

      This article includes 19 references
      1. Afshar A, Dulac G C, Wright P F, Martin D. Application of indirect ELISA for detection of bovine antibodies against vesicular stomatitis viruses.. J Vet Diagn Investig 1993;5:26–32.
        pubmed: 8385501
      2. Afshar A, Shakarchi H, Dulac G C. Development of a competitive enzyme-linked immunosorbent assay for detection of bovine, ovine, porcine, and equine antibodies to vesicular stomatitis virus.. J Clin Microbiol 1993;31:1860–1865.
        pmc: PMC265646pubmed: 8394377
      3. Altin J G, Pagler E B. A one-step procedure for biotinylation and chemical cross linking of lymphocyte surface and intracellular membrane-associated molecules.. Anal Biochem 1995;224:382–389.
        pubmed: 7710096
      4. Boulanger P. Complement-fixation tests of swine serum. I. In the diagnosis of vesicular stomatitis.. Can J Comp Med 1955;19:37–47.
        pmc: PMC1614046pubmed: 17648785
      5. Brown F, Bishop D H L, Crick J. Rhabdoviridae.. Intervirol 1979;12:1–17.
        pubmed: 500329
      6. Carayannopoulos L, Capra I D. Immunoglobulins: structure and function.. .
      7. Hanson R P. The natural history of vesicular stomatitis.. Bacteriol Rev 1952;16:179–204.
        pmc: PMC180742pubmed: 12987386
      8. Hanson R P. Vesicular stomatitis.. .
      9. Karstad L H, Adams E V, Hanson R P, Ferris D H. Evidence for the role of wildlife in epizootics of vesicular stomatitis.. J Am Vet Med Assoc 1956;129:95–96.
        pubmed: 13345692
      10. Katz J B, Eernisse K A, Landgraf J G, Schmitt B J. Comparative performance of four serodiagnostic procedures for detecting bovine and equine vesicular stomatitis virus antibodies.. J Vet Diagn Investig 1997;9:329–331.
        pubmed: 9249179
      11. Lowery O H, Rosebrough N J, Farr A L, Randall R J. Protein measurement with the Folin phenol reagent.. J Biol Chem 1951;193:265–275.
        pubmed: 14907713
      12. Nielsen K H, Wright P F, Nielsen E B. Enzyme immunoassay: application to the detection of bovine antibody to Brucella abortus.. Mo: Agriculture Canada; 1984. nograph.
      13. Office International des Epizooties. Manual of standards for diagnostic tests and vaccines.. Paris, France: Office International Des Epizooties, World Organisation for Animal Health; 1996. p. 57.
      14. Snyder M L, Jenney E W, Erickson G A, Carbrey E A. The 1982 resurgence of vesicular stomatitis in the United States; a summary of laboratory diagnostic findings.. Proc Annu Meet Am Assoc Vet Lab Diagn 1982;25:221–228.
      15. Tesh R B, Da Rosa A P A, Travassos D R, Travassos J S. Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates.. J Gen Virol 1983;64:169–176.
        pubmed: 6337233
      16. Vernon S D, Webb P A. Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.. J Clin Microbiol 1985;22:582–586.
        pmc: PMC268471pubmed: 3001132
      17. Webb P A, Holbrook F R. Vesicular stomatitis.. Boca Raton, Fla: CRC Press, Inc.; 1989. pp. 1–29.
      18. Wilks C R, House J A. The glycoproteins of seven vesiculoviruses are antigenically distinct.. Arch Virol 1985;86:335–340.
        pubmed: 4062563
      19. Yuill T M. Vesicular stomatitis.. Boca Raton, Fla: CRC Press, Inc.; 1981. pp. 125–142.

      Citations

      This article has been cited 2 times.
      1. Lee HS, Heo EJ, Jeoung HY, Ko HR, Kweon CH, Youn HJ, Ko YJ. Enzyme-linked immunosorbent assay using glycoprotein and monoclonal antibody for detecting antibodies to vesicular stomatitis virus serotype New Jersey. Clin Vaccine Immunol 2009 May;16(5):667-71.
        doi: 10.1128/CVI.00043-09pubmed: 19279165google scholar: lookup
      2. Burnouf T, Griffiths E, Padilla A, Seddik S, Stephano MA, Gutiérrez JM. Assessment of the viral safety of antivenoms fractionated from equine plasma. Biologicals 2004 Sep;32(3):115-28.
        doi: 10.1016/j.biologicals.2004.07.001pubmed: 15536042google scholar: lookup
      Subscribe to our newsletter
      Join 99,850 horse owners like you who receive our email updates on horse health and nutrition.