Development of loop-mediated isothermal amplification (LAMP) for detection of Theileria equi.
Abstract: Several approaches have been developed for diagnosis of Theileria equi infection in horses and donkeys but all of them have limitations in practice. Due to numerous strengths including easy operation, cheapness and high sensitivity and specificity, LAMP has been already extensively used for surveillance of a number of diseases. We here set up a LAMP assay based on 18S rRNA gene for T. equi diagnosis. The approach was specific enough to differentiate T. equi from other evolutionary-related protozoa. Moreover, it was sensitive enough that LAMP was capable of detecting as much low as 10 copy target gene and 1 pg/μl blood genomic DNA. It was further demonstrated that LAMP was much more sensitive than canonical blood smear and comparable to PCR using test and field samples. The present results support an idea that LAMP developed in this study is reliable, reproducible and highly sensitive and specific, being a potential to be globally used for surveillance of T. equi infection in the field.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-05-24 PubMed ID: 23711610DOI: 10.1016/j.actatropica.2013.05.007Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study focuses on developing and testing a new method for diagnosing Theileria equi infections in horses and donkeys using loop-mediated isothermal amplification (LAMP), which turned out to be reliable, reproducible, and highly sensitive and specific in detecting the infection compared to existing methods.
Loop-Mediated Isothermal Amplification (LAMP)
- This research introduces another approach to detecting Theileria equi infection called loop-mediated isothermal amplification (LAMP), complements other detection techniques but provides more advantages, such as cost effectiveness and ease of operation.
- LAMP, having been used throughout various disease surveillance activities owing to its numerous strengths, is the main focus of this article. The authors focus on leveraging these benefits to improve the detection process for Theileria equi infection.
- In this study, LAMP is set up based on the 18S rRNA gene, which is specifically related to T. equi.
Significance and Reliability of LAMP
- The researchers claim that the developed LAMP approach is specific enough to differentiate T. equi from similar protozoa, making it a significant development in Theileria equi infection detection.
- The sensitivity of LAMP is also recognized in the study, with the ability to detect as low as 10 copy target genes and 1 picogram per microliter of blood genomic DNA. This gives LAMP the power to detect the disease even at very early stages or in low presence, proving its efficiency.
Comparisons with Other Diagnostic Methods
- The article goes on to compare LAMP with other traditional detection methods such as canonical blood smear and polymerase chain reaction (PCR) diagnostics. The results highlight LAMP’s higher sensitivity compared to blood smear and competitors with PCR, reinforcing its viability as an alternative diagnostic approach.
- Considering both the field and test samples, LAMP provides a higher rate of infection detection, further strengthening its applicability and practicality in the field.
Potential Global Use for Surveillance
- Finally, the authors suggest that due to its reproducibility, reliability, and high sensitivity and specificity, the LAMP technique developed in the study has the potential to be globally used for Theileria equi infection surveillance in the field.
- This global applicability means it could be a game-changer for diagnosing and controlling the spread of this infection in both horses and donkeys worldwide, leading to better health outcomes for these animals.
Cite This Article
APA
Xie J, Liu G, Tian Z, Luo J.
(2013).
Development of loop-mediated isothermal amplification (LAMP) for detection of Theileria equi.
Acta Trop, 127(3), 245-250.
https://doi.org/10.1016/j.actatropica.2013.05.007 Publication
Researcher Affiliations
- State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu, China.
MeSH Terms
- Animals
- Equidae
- Nucleic Acid Amplification Techniques / methods
- Reproducibility of Results
- Sensitivity and Specificity
- Theileria / classification
- Theileria / isolation & purification
- Theileriasis / blood
- Theileriasis / diagnosis
Citations
This article has been cited 6 times.- Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
- Lei R, Wang X, Zhang D, Liu Y, Chen Q, Jiang N. Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis. Sci Rep 2020 Mar 5;10(1):4096.
- Mans BJ, Pienaar R, Latif AA. A review of Theileria diagnostics and epidemiology. Int J Parasitol Parasites Wildl 2015 Apr;4(1):104-18.
- Xia Y, Guo XG, Zhou S. Rapid detection of Streptococcus pneumoniae by real-time fluorescence loop-mediated isothermal amplification. J Thorac Dis 2014 Sep;6(9):1193-9.
- Chen Z, Liu Q, Jiao FC, Xu BL, Zhou XN. Detection of piroplasms infection in sheep, dogs and hedgehogs in Central China. Infect Dis Poverty 2014;3:18.
- Sun H, Fan J, Chu H, Gao Y, Fang J, Wu Q, Ding H, Zhuo X, Kong Q, Lv H, Zheng B, Lu S. RPA-CRISPR/Cas12a-LFA combined with a digital visualization instrument to detect Toxoplasma gondii in stray dogs and cats in Zhejiang province, China. Microbiol Spectr 2024 Jul 2;12(7):e0399823.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
