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Journal of parasitic diseases : official organ of the Indian Society for Parasitology2019; 44(1); 99-104; doi: 10.1007/s12639-019-01166-8

Development of membrane-based flow-through assay for detection of trypanosomosis in equines.

Abstract: A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti- antibodies in equine sera and can be used for quick screening of serum samples under field conditions.
© Indian Society for Parasitology 2019.
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Publication Date: 2019-11-08 PubMed ID: 32174710PubMed Central: PMC7046882DOI: 10.1007/s12639-019-01166-8Google Scholar: Lookup
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Summary

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This research article describes the development and testing of a fast flow-through immunological test for detecting trypanosomosis, a parasitic disease, in horses. Using colored gold nanoparticles, the test identifies disease-specific antibodies in a horse’s blood within 14 days of infection and takes less than 5 minutes to conduct.

Development of the Immunoassay

  • The study presents the development of a rapid flow-through immunological test (often known as an immunoassay) to detect the presence of trypanosomosis, a disease caused by parasites, in equines (horses, donkeys, etc.).
  • Crucial to this method is the use of protein A labeled gold nanoparticles (GNPs). These GNPs act as a visually detectable marker when antibodies specific to trypanosomosis are present in equine serum samples.

Functioning of the Immunoassay

  • The immunoassay device incorporates an antigen-coated nitrocellulose membrane secured on absorbent pads. When a serum sample is introduced, the flow-through device enables the specific disease-causing antibodies, if present in the sample, to bind with the coated antigens.
  • The GNPs, being labeled with protein A (which binds to antibodies), then attach to these antibody-antigen pairs causing a visible color change. This acts as an indicator of infection.

Efficiency and Speed of the Immunoassay

  • The developed immunoassay can detect specific trypanosomosis antibodies as early as 14 days post-infection. This timely detection is beneficial in managing and preventing the spread of this disease.
  • The entire assay process can be conducted in less than 5 minutes, which is highly advantageous in quick screenings, particularly under field conditions.

Comparable Results with Other Methods

  • The results of this flow-through assay have been found to be comparable with other conventional methods such as ELISA (Enzyme-Linked Immunosorbent Assay), dot blot assay, and lateral flow assay. This implies a high reliability and validity of this developed rapid test procedure.
  • Again, the high efficiency of this assay in detecting antibodies in equine serum illustrates its potential to become a significant diagnostic tool, particularly for field testing.

Cite This Article

APA
Kumar R, Yadav SC, Kumar S, Dilbaghi N. (2019). Development of membrane-based flow-through assay for detection of trypanosomosis in equines. J Parasit Dis, 44(1), 99-104. https://doi.org/10.1007/s12639-019-01166-8

Publication

Journal of parasitic diseases : official organ of the Indian Society for Parasitology
ISSN: 0971-7196
NlmUniqueID: 9713059
Country: India
Language: English
Volume: 44
Issue: 1
Pages: 99-104

Researcher Affiliations

Kumar, Ritesh
  • 1Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001 India.
Yadav, Suresh Chandra
  • 2National Research Centre on Equines, Hisar, Haryana 125001 India.
Kumar, Sandeep
  • 1Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001 India.
Dilbaghi, Neeraj
  • 1Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, 125001 India.

Conflict of Interest Statement

Conflict of interestAll authors declare that they have no conflict of interest.

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Citations

This article has been cited 2 times.
  1. Kaur M, Eltzov E. Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection of Escherichia coli. Biosensors (Basel) 2022 Jan 25;12(2).
    doi: 10.3390/bios12020063pubmed: 35200324google scholar: lookup
  2. Di Nardo F, Chiarello M, Cavalera S, Baggiani C, Anfossi L. Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives. Sensors (Basel) 2021 Jul 30;21(15).
    doi: 10.3390/s21155185pubmed: 34372422google scholar: lookup
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