Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA.
Abstract: Recently, a type-specific ELISA using equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) glycoprotein Gs (gGs) was developed by Crabb and Studdert [1993]. To investigate the dissemination of EHV-1 and -4 among horses in Japan, we applied their ELISA as suitable for discriminating between EHV-1 and -4 infections serologically. Type-specificity of the ELISA was confirmed by using paired sera of infected horses with either EHV-1 or -4. Application of the ELISA to sera collected before and after the winter season of 1995-1996 from 80 racehorses revealed that 30 horses showed significant antibody responses against EHV-1 and 9 against EHV-4, respectively. The results indicated that this ELISA using paired sera is useful for a diagnosis and an epizootiological study on EHV-1 and -4 infections.
Publication Date: 1998-11-20 PubMed ID: 9819768DOI: 10.1292/jvms.60.1133Google Scholar: Lookup
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Summary
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The research article discusses the use of a type-specific ELISA test for detecting and understanding the spread of equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) among horses in Japan.
Research Methodology
- Researchers used a type-specific ELISA test that had been developed by Crabb and Studdert in 1993. This test uses glycoprotein Gs (gGs) from both EHV-1 and EHV-4 to discriminate between the two viruses serologically.
- The type-specificity of the ELISA test was validated using ‘paired sera’, which are two or more serum samples taken from the same horse at different times. This was done on horses previously infected with either EHV-1 or EHV-4, thus examining the test’s capacity to identify the specific type of virus.
Study Application and Findings
- The researchers applied the ELISA test to serum samples collected from 80 racehorses in Japan. The samples were collected before and after the winter season of 1995-1996.
- The results demonstrated that 30 horses had significant antibody responses to EHV-1, and 9 had significant antibody responses to EHV-4. Antibody responses indicate that the horses were exposed to the viruses, as antibodies are proteins that the body produces to fight off infections.
Conclusion
- The results of this study validates the use of the ELISA test using paired sera for diagnosing and conducting an epizootiological study. Epizootiology is the study of disease occurrence and its transmission among animal populations.
- The research therefore indicated that the ELISA test could be useful in detecting and studying the disseminations of EHV-1 and EHV-4 infections amongst horse population.
Cite This Article
APA
Yasunaga S, Maeda K, Matsumura T, Kai K, Iwata H, Inoue T.
(1998).
Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA.
J Vet Med Sci, 60(10), 1133-1137.
https://doi.org/10.1292/jvms.60.1133 Publication
Researcher Affiliations
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
MeSH Terms
- Animals
- Antibodies, Viral / biosynthesis
- Antibodies, Viral / blood
- Enzyme-Linked Immunosorbent Assay / veterinary
- Female
- Fever / complications
- Fever / veterinary
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / epidemiology
- Herpesviridae Infections / veterinary
- Herpesvirus 1, Equid / immunology
- Horse Diseases / diagnosis
- Horse Diseases / epidemiology
- Horses
- Japan / epidemiology
- Male
- Varicellovirus / immunology
- Viral Envelope Proteins / immunology
Citations
This article has been cited 9 times.- El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations.. Animals (Basel) 2021 Sep 29;11(10).
- Bannai H, Tsujimura K, Nemoto M, Ohta M, Yamanaka T, Kokado H, Matsumura T. Epizootiological investigation of equine herpesvirus type 1 infection among Japanese racehorses before and after the replacement of an inactivated vaccine with a modified live vaccine.. BMC Vet Res 2019 Aug 6;15(1):280.
- Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Maeda K, Kondo T. Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.. J Vet Med Sci 2016 Feb;78(2):309-11.
- Abdelgawad A, Hermes R, Damiani A, Lamglait B, Czirják GÁ, East M, Aschenborn O, Wenker C, Kasem S, Osterrieder N, Greenwood AD. Comprehensive Serology Based on a Peptide ELISA to Assess the Prevalence of Closely Related Equine Herpesviruses in Zoo and Wild Animals.. PLoS One 2015;10(9):e0138370.
- Bannai H, Mae N, Ode H, Nemoto M, Tsujimura K, Yamanaka T, Kondo T, Matsumura T. Successful control of winter pyrexias caused by equine herpesvirus type 1 in Japanese training centers by achieving high vaccination coverage.. Clin Vaccine Immunol 2014 Aug;21(8):1070-6.
- Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kondo T, Matsumura T. Improving a Complement-fixation Test for Equine Herpesvirus Type-1 by Pretreating Sera with Potassium Periodate to Reduce Non-specific Hemolysis.. J Equine Sci 2013;24(4):71-4.
- Ohta M, Nemoto M, Tsujimura K, Kondo T, Matsumura T. Evaluation of the usefulness of a PCR assay performed at a clinical laboratory for the diagnosis of respiratory disease induced by equine herpesvirus type 1 in the field.. J Equine Sci 2011;22(3):53-6.
- Maeda K, Mizukoshi F, Hamano M, Kai K, Kondo T, Matsumura T. Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.. Clin Diagn Lab Immunol 2005 Jan;12(1):122-4.
- Maeda K, Mizukoshi F, Hamano M, Kai K, Iwata H, Kondo T, Matsumura T. Development of an equine herpesvirus type 4-specific enzyme-linked immunosorbent assay using a B-cell epitope as an antigen.. J Clin Microbiol 2004 Mar;42(3):1095-8.
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