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Home / Equine Research Bank / Life (Basel) / Dietary Supplementation with Boswellia serrata, Verbascum th...
Life (Basel, Switzerland)2023; 13(3); doi: 10.3390/life13030750

Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression.

Abstract: Intense exercise can cause inflammation and oxidative stress due to the production of reactive oxygen species. These pathophysiological processes are interdependent, and each one can induce the other, creating a vicious circle. A placebo-controlled blind study was carried out in show jumping horses (n. 16) to evaluate the effects of a commercial dietary supplement (Dolhorse® N.B.F. Lanes srl, Milan, Italy) containing Verbascum thapsus leaf powder (1.42%), Curcuma longa (14.280 mg/kg), and Boswellia serrata (Roxb ex Colebr) (14.280 mg/kg) extracts. Before and after 10 days of dietary supplementation, blood samples were collected to evaluate the protein levels, antioxidants, and inflammatory responses by proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (real-time RT-PCR). A total of 36 protein spots, connected to 29 proteins, were modulated by dietary supplementation, whereas real-time RT-PCR revealed a significant downregulation of proinflammatory cytokines (interleukin 1α (p < 0.05) and interleukin-6 (0.005), toll-like receptor 4 (p < 0.05), and IKBKB (p < 0.05) in supplemented sport horses. Immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein AIV, and filamin B were overexpressed, whereas haptoglobin, α-2-HS-glycoprotein, α2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced. No effect was observed on the antioxidant defense systems. The present results suggest this phytotherapy may reinforce the innate immune responses, thus representing a valid adjuvant to alleviate inflammation, which is a pathophysiological process in sport horses.
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Publication Date: 2023-03-10 PubMed ID: 36983904PubMed Central: PMC10055707DOI: 10.3390/life13030750Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article examines the impact of certain dietary supplements on the health of show jumping horses, with a specific focus on inflammation and oxidative stress caused by intense physical exercise.

Research Aim and Methodology

  • The study aims to evaluate the effects of a commercial dietary supplement containing Verbascum thapsus leaf powder, Curcuma longa, and Boswellia serrata extracts on intense exercise-induced inflammation and oxidative stress in show jumping horses.
  • Researchers employed a placebo-controlled blind study involving 16 show jumping horses.
  • Statistics were sampled before and after ten days of supplementing the horses’ diets, with the gathered data including protein levels, antioxidant measures, and inflammatory responses. These metrics were all gauged through proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).

Results

  • Supplementing the horses’ diets resulted in the modulation of 36 protein spots related to 29 proteins.
  • Real-time RT-PCR revealed a significant decrease in proinflammatory cytokines (interleukin 1α and interleukin-6), toll-like receptor 4, and IKBKB in the supplemented horses.
  • Several proteins like immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein IV, and filamin B were overexpressed post supplementation.
  • At the same time, proteins such as haptoglobin, α-2-HS-glycoprotein, α2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced.
  • There was no noticeable impact on antioxidant defense systems.

Conclusion

  • The findings from this study suggest that the dietary supplement could potentially fortify the horses’ innate immune responses, representing a valuable addition to mitigate inflammation, a pathophysiological behavior associated with sports horses.
  • However, the study did not notably affect the horses’ antioxidant defense systems.

Overall, this research could have implications for the welfare and performance of sport horses around the world. Further research may be necessary to confirm the findings and fully understand the effects of these dietary supplements on antioxidant defense systems.

Cite This Article

APA
Beghelli D, Zallocco L, Angeloni C, Bistoni O, Ronci M, Cavallucci C, Mazzoni MR, Nuccitelli A, Catalano C, Hrelia S, L쫌hini A, Giusti L. (2023). Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression. Life (Basel), 13(3). https://doi.org/10.3390/life13030750

Publication

Life (Basel, Switzerland)
ISSN: 2075-1729
NlmUniqueID: 101580444
Country: Switzerland
Language: English
Volume: 13
Issue: 3

Researcher Affiliations

Beghelli, Daniela
  • School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Zallocco, Lorenzo
  • Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy.
Angeloni, Cristina
  • Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy.
Bistoni, Onelia
  • Rheumatology Unit, Department of Medicine, University of Perugia, 06126 Perugia, Italy.
Ronci, Maurizio
  • Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, Italy.
Cavallucci, Clarita
  • Independent Researcher, 06083 Perugia, Italy.
Mazzoni, Maria Rosa
  • Department of Pharmacy, University of Pisa, 56126 Pisa, Italy.
Nuccitelli, Anna
  • School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Catalano, Chiara
  • Independent Researcher, 36023 Vicenza, Italy.
Hrelia, Silvana
  • Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy.
L쫌hini, Antonio
  • Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy.
Giusti, Laura
  • School of Pharmacy, University of Camerino, 62032 Camerino, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 90 references
  1. Sjödin B, Westing YH, Apple FS. Biochemical Mechanisms for Oxygen Free Radical Formation During Exercise. Sports Med 1990;10:236–254.
    doi: 10.2165/00007256-199010040-00003pubmed: 2247725google scholar: lookup
  2. Arfuso F, Rizzo M, Giannetto C, Giudice E, Cirincione R, Cassata G, Cicero L, Piccione G. Oxidant and Antioxidant Parameters’ Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise. Antioxidants 2022;11:1176.
    doi: 10.3390/antiox11061176pmc: PMC9220350pubmed: 35740073google scholar: lookup
  3. Williams CA. HORSE SPECIES SYMPOSIUM: The Effect of Oxidative Stress during Exercise in the Horse1. J. Anim. Sci. 2016;94:4067–4075.
    doi: 10.2527/jas.2015-9988pubmed: 27898872google scholar: lookup
  4. Shono S, Gin A, Minowa F, Okubo K, Mochizuki M. The Oxidative Stress Markers of Horses—The Comparison with Other Animals and the Influence of Exercise and Disease. Animals 2020;10:617.
    doi: 10.3390/ani10040617pmc: PMC7222798pubmed: 32260122google scholar: lookup
  5. Chiaradia E, Avellini L, Rueca F, Spaterna A, Porciello F, Antonioni MT, Gaiti A. Physical Exercise, Oxidative Stress and Muscle Damage in Racehorses. Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 1998;119:833–836.
    doi: 10.1016/S0305-0491(98)10001-9pubmed: 9787774google scholar: lookup
  6. Clarkson PM, Thompson HS. Antioxidants: What Role Do They Play in Physical Activity and Health?. Am. J. Clin. Nutr. 2000;72:637S–646S.
    doi: 10.1093/ajcn/72.2.637Spubmed: 10919970google scholar: lookup
  7. Kinnunen S., Atalay M., Hyyppä S., Lehmuskero A., Hänninen O., Oksala N. Effects of Prolonged Exercise on Oxidative Stress and Antioxidant Defense in Endurance Horse. J Sports Sci. Med. 2005;4:415–421.
    pmc: PMC3899657pubmed: 24501555
  8. Kinnunen S, Hyyppä S, Lappalainen J, Oksala N, Venojärvi M, Nakao C, Hänninen O, Sen CK, Atalay M. Exercise-Induced Oxidative Stress and Muscle Stress Protein Responses in Trotters. Eur. J. Appl. Physiol. 2005;93:496–501.
    doi: 10.1007/s00421-004-1162-xpubmed: 15221402google scholar: lookup
  9. Urso ML, Clarkson PM. Oxidative Stress, Exercise, and Antioxidant Supplementation. Toxicology 2003;189:41–54.
    doi: 10.1016/S0300-483X(03)00151-3pubmed: 12821281google scholar: lookup
  10. Clarkson PM, Hubal MJ. Exercise-Induced Muscle Damage in Humans. Am. J. Phys. Med. Rehabilitation. 2002;81:S52–S69.
    doi: 10.1097/00002060-200211001-00007pubmed: 12409811google scholar: lookup
  11. Malm C. Exercise-Induced Muscle Damage and Inflammation: Fact or Fiction?: Exercise Induced Muscle Inflammation. Acta Physiol. Scand. 2001;171:233–239.
    doi: 10.1046/j.1365-201x.2001.00825.xpubmed: 11412135google scholar: lookup
  12. Febbraio MA, Pedersen BK. Muscle-derived Interleukin-6: Mechanisms for Activation and Possible Biological Roles. FASEB J. 2002;16:1335–1347.
    doi: 10.1096/fj.01-0876revpubmed: 12205025google scholar: lookup
  13. Arent SM, Senso M, Golem DL, McKeever KH. The Effects of Theaflavin-Enriched Black Tea Extract on Muscle Soreness, Oxidative Stress, Inflammation, and Endocrine Responses to Acute Anaerobic Interval Training: A Randomized, Double-Blind, Crossover Study. J Int. Soc. Sports Nutr. 2010;7:11.
    doi: 10.1186/1550-2783-7-11pmc: PMC2837615pubmed: 20178632google scholar: lookup
  14. Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive Oxygen Species in Inflammation and Tissue Injury. Antioxid. Redox Signal. 2014;20:1126–1167.
    doi: 10.1089/ars.2012.5149pmc: PMC3929010pubmed: 23991888google scholar: lookup
  15. Biswas SK. Does the Interdependence between Oxidative Stress and Inflammation Explain the Antioxidant Paradox?. Oxid. Med. Cell. Longev. 2016;2016:1–9.
    doi: 10.1155/2016/5698931pmc: PMC4736408pubmed: 26881031google scholar: lookup
  16. Djuric Z, Kashif M, Fleming T, Muhammad S, Piel D, von Bauer R, Bea F, Herzig S, Zeier M, Pizzi M. Targeting Activation of Specific NF-ΚB Subunits Prevents Stress-Dependent Atherothrombotic Gene Expression. Mol. Med. 2012;18:1375–1386.
    doi: 10.2119/molmed.2012.00282pmc: PMC3533647pubmed: 23114885google scholar: lookup
  17. Kirschvink N, Art T, Moffarts B, Smith N, Marlin D, Roberts C, Lekeux P. Relationship between Markers of Blood Oxidant Status and Physiological Variables in Healthy and Heaves-Affected Horses after Exercise. Equine Vet. J. 2010;34:159–164.
    doi: 10.1111/j.2042-3306.2002.tb05410.xpubmed: 12405678google scholar: lookup
  18. El-Ashker M, El-Khodery S, Metwally N, Hussein H, El-Boshy M. Prognostic Significance of Oxidative Stress Markers in Colitis Associated with Phenylbutazone Administration in Draft Horses. J. Equine Vet. Sci. 2012;32:146–152.
    doi: 10.1016/j.jevs.2011.08.008google scholar: lookup
  19. Molinari L, Basini G, Ramoni R, Bussolati S, Aldigeri R, Grolli S, Bertini S, Quintavalla F. Evaluation of Oxidative Stress Parameters in Healthy Saddle Horses in Relation to Housing Conditions, Presence of Stereotypies, Age, Sex and Breed. Processes 2020;8:1670.
    doi: 10.3390/pr8121670google scholar: lookup
  20. Smarsh DN, Williams CA. Oxidative Stress and Antioxidant Status in Standardbreds: Effect of Age and Acute Exercise Before and After Training. J. Equine Vet. Sci. 2016;47:92–106.
    doi: 10.1016/j.jevs.2016.07.019google scholar: lookup
  21. Williams CA, Kronfeld DS, Hess TM, Saker KE, Waldron JN, Crandell KM, Hoffman RM, Harris PA. Antioxidant Supplementation and Subsequent Oxidative Stress of Horses during an 80-Km Endurance Race1. J. Anim. Sci. 2004;82:588–594.
    doi: 10.2527/2004.822588xpubmed: 14974559google scholar: lookup
  22. Marañón G, Muñoz-Escassi B, Manley W, García C, Cayado P, de la Muela MS, Olábarri B, León R, Vara E. The Effect of Methyl Sulphonyl Methane Supplementation on Biomarkers of Oxidative Stress in Sport Horses Following Jumping Exercise. Acta Vet. Scand. 2008;50:45.
    doi: 10.1186/1751-0147-50-45pmc: PMC2586020pubmed: 18992134google scholar: lookup
  23. Ememe MU, Mshelia WP, Ayo JO. Ameliorative Effects of Resveratrol on Oxidative Stress Biomarkers in Horses. J. Equine Vet. Sci. 2015;35:518–523.
    doi: 10.1016/j.jevs.2015.02.006google scholar: lookup
  24. Kienzle E, Freismuth A, Reusch A. Double-Blind Placebo-Controlled Vitamin E or Selenium Supplementation of Sport Horses with Unspecified Muscle Problems. An Example of the Potential of Placebos. J. Nutr. 2006;136:2045S–2047S.
    doi: 10.1093/jn/136.7.2045Spubmed: 16772492google scholar: lookup
  25. Nemec Svete A, Vovk T, Bohar Topolovec M, Kruljc P. Effects of Vitamin E and Coenzyme Q10 Supplementation on Oxidative Stress Parameters in Untrained Leisure Horses Subjected to Acute Moderate Exercise. Antioxidants 2021;10:908.
    doi: 10.3390/antiox10060908pmc: PMC8227526pubmed: 34205129google scholar: lookup
  26. White A, Estrada M, Walker K, Wisnia P, Filgueira G, Valdés F, Araneda O, Behn C, Martínez R. Role of Exercise and Ascorbate on Plasma Antioxidant Capacity in Thoroughbred Race Horses. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 2001;128:99–104.
    doi: 10.1016/S1095-6433(00)00286-5pubmed: 11137442google scholar: lookup
  27. Yonezawa LA, Machado LP, Silveira VFD, Watanabe MJ, Saito ME, Kitamura SS, Kohayagawa A. Malondialdeído e Troponina I Cardíaca Em Equinos Da Raça Puro Sangue Árabe Submetidos Ao Exercício e à Suplementação Com Vitamina E. Ciência Rural 2010;40:1321–1326.
    doi: 10.1590/S0103-84782010005000094google scholar: lookup
  28. Elghandour MMMY, Kanth Reddy PR, Salem AZM, Ranga Reddy PP, Hyder I, Barbabosa-Pliego A, Yasaswini D. Plant Bioactives and Extracts as Feed Additives in Horse Nutrition. J. Equine Vet. Sci. 2018;69:66–77.
    doi: 10.1016/j.jevs.2018.06.004google scholar: lookup
  29. Dockalova H, Baholet D, Batik A, Zeman L, Horky P. Effect of Milk Thistle (Silybum Marianum) Seed Cakes by Horses Subjected to Physical Exertion. J. Equine Vet. Sci. 2022;113:103937.
    doi: 10.1016/j.jevs.2022.103937pubmed: 35318098google scholar: lookup
  30. Tsuda T. Curcumin as a Functional Food-Derived Factor: Degradation Products, Metabolites, Bioactivity, and Future Perspectives. Food Funct. 2018;9:705–714.
    doi: 10.1039/C7FO01242Jpubmed: 29206254google scholar: lookup
  31. Nelson KM, Dahlin JL, Bisson J, Graham J, Pauli GF, Walters MA. The Essential Medicinal Chemistry of Curcumin: Miniperspective. J. Med. Chem. 2017;60:1620–1637.
    doi: 10.1021/acs.jmedchem.6b00975pmc: PMC5346970pubmed: 28074653google scholar: lookup
  32. Ashrafizadeh M, Ahmadi Z, Mohammadinejad R, Farkhondeh T, Samarghandian S. Curcumin Activates the Nrf2 Pathway and Induces Cellular Protection Against Oxidative Injury. Curr. Mol. Med. 2020;20:116–133.
    doi: 10.2174/1566524019666191016150757pubmed: 31622191google scholar: lookup
  33. Menon VP, Sudheer AR. Antioxidant and anti-inflammatory properties of curcumin. 2007. In: Aggarwal BB, Surh Y-J, Shishodia S, editors. The Molecular Targets and Therapeutic Uses of Curcumin in Health and Disease. Volume 595. Springer; Boston, MA, USA. pp. 105–125. Advances in Experimental Medicine and Biology.
    pubmed: 17569207
  34. Andrews FM, Riggs LM, Lopez MJ, Keowen ML, Garza F, Takawira C, Liu C-C, Liu Y, Seeram NP, Cairy A. Effect of an Oral Supplement Containing Curcumin Extract (Longvida ® ) on Lameness Due to Osteoarthritis and Gastric Ulcer Scores. Equine Vet. Educ. 2022:eve.13616.
    doi: 10.1111/eve.13616google scholar: lookup
  35. Wuest S, Atkinson RL, Bland SD, Hastings D. A Pilot Study on the Effects of Curcumin on Parasites, Inflammation, and Opportunistic Bacteria in Riding Horses. J. Equine Vet. Sci. 2017;57:46–50.
    doi: 10.1016/j.jevs.2017.06.010google scholar: lookup
  36. Innes JF, Fuller CJ, Grover ER, Kelly AL, Burn JF. Randomised, Double-Blind, Placebocontrolled Parallel Group Study of P54FP for the Treatment of Dogs with Osteoarthritis. Vet. Rec. 2003;152:457–460.
    doi: 10.1136/vr.152.15.457pubmed: 12723628google scholar: lookup
  37. Kulkarni RR, Patki PS, Jog VP, Gandage SG, Patwardhan B. Treatment of Osteoarthritis with a Herbomineral Formulation: A Double-Blind, Placebo-Controlled, Cross-over Study. J. Ethnopharmacol. 1991;33:91–95.
    doi: 10.1016/0378-8741(91)90167-Cpubmed: 1943180google scholar: lookup
  38. Starzonek J, Roscher K, Blüher M, Blaue D, Schedlbauer C, Hirz M, Raila J, Vervuert I. Effects of a Blend of Green Tea and Curcuma Extract Supplementation on Lipopolysaccharide-Induced Inflammation in Horses and Ponies. PeerJ 2019;7:e8053.
    doi: 10.7717/peerj.8053pmc: PMC6857679pubmed: 31741800google scholar: lookup
  39. Siddiqui MZ. Boswellia Serrata, a Potential Antiinflammatory Agent: An Overview. Indian J. Pharm. Sci. 2011;73:255–261.
    doi: 10.4103/0250-474X.93507pmc: PMC3309643pubmed: 22457547google scholar: lookup
  40. Hamm S, Bleton J, Connan J, Tchapla A. A Chemical Investigation by Headspace SPME and GC–MS of Volatile and Semi-Volatile Terpenes in Various Olibanum Samples. Phytochemistry 2005;66:1499–1514.
    doi: 10.1016/j.phytochem.2005.04.025pubmed: 15922374google scholar: lookup
  41. Ammon HPT. Salai Guggal—Boswellia Serrata: From a Herbal Medicine to a Specific Inhibitor of Leukotriene Biosynthesis. Phytomedicine 1996;3:67–70.
    doi: 10.1016/S0944-7113(96)80012-2pubmed: 23194863google scholar: lookup
  42. Al-Yasiry ARM, Kiczorowska B. Frankincense–Therapeutic Properties. Postepy. Hig. Med. Dosw. 2016;70:380–391.
    doi: 10.5604/17322693.1200553pubmed: 27117114google scholar: lookup
  43. Umar S, Umar K, Sarwar AHMG, Khan A, Ahmad N, Ahmad S, Katiyar CK, Husain SA, Khan HA. Boswellia Serrata Extract Attenuates Inflammatory Mediators and Oxidative Stress in Collagen Induced Arthritis. Phytomedicine 2014;21:847–856.
    doi: 10.1016/j.phymed.2014.02.001pubmed: 24667331google scholar: lookup
  44. Franceschi F, Togni S, Belcaro G, Dugall M, Luzzi R, Ledda A, Pellegrini L, Eggenhoffner R, Giacomelli L. A Novel Lecithin Based Delivery Form of Boswellic Acids (Casperome®) for the Management of Osteo-Muscular Pain: A Registry Study in Young Rugby Players. Eur. Rev. Med. Pharmacol. Sci. 2016;20:4156–4161.
    pubmed: 27775780
  45. Chilelli N, Ragazzi E, Valentini R, Cosma C, Ferraresso S, Lapolla A, Sartore G. Curcumin and Boswellia Serrata Modulate the Glyco-Oxidative Status and Lipo-Oxidation in Master Athletes. Nutrients 2016;8:745.
    doi: 10.3390/n脐745pmc: PMC5133128pubmed: 27879642google scholar: lookup
  46. Speranza L, Franceschelli S, Pesce M, Reale M, Menghini L, Vinciguerra I, De Lutiis MA, Felaco M, Grilli A. Antiinflammatory Effects in THP-1 Cells Treated with Verbascoside: ANTIINFLAMMATORY ACTION IN THP-1 CELLS OF VERBASCOSIDE. Phytotherapy Res. 2010;24:1398–1404.
    doi: 10.1002/ptr.3173pubmed: 20812283google scholar: lookup
  47. Turker AU, Camper ND. Biological Activity of Common Mullein, a Medicinal Plant. J.Ethnopharmacol. 2002;82:117–125.
    doi: 10.1016/S0378-8741(02)00186-1pubmed: 12241986google scholar: lookup
  48. Calabrese G, Zappalà A, Dolcimascolo A, Acquaviva R, Parenti R, Malfa GA. Phytochemical Analysis and Anti-Inflammatory and Anti-Osteoarthritic Bioactive Potential of Verbascum Thapsus L. (Scrophulariaceae) Leaf Extract Evaluated in Two In Vitro Models of Inflammation and Osteoarthritis. Molecules 2021;26:5392.
    doi: 10.3390/molecules26175392pmc: PMC8434610pubmed: 34500824google scholar: lookup
  49. Lans C, Turner N, Brauer G, Lourenco G, Georges K. Ethnoveterinary Medicines Used for Horses in Trinidad and in British Columbia, Canada. J Ethnobiol. Ethnomedicine. 2006;2:31.
    doi: 10.1186/1746-4269-2-31pmc: PMC1559680pubmed: 16893454google scholar: lookup
  50. Carroll CL, Huntington PJ. Body Condition Scoring and Weight Estimation of Horses. Equine Vet. J. 1988;20:41–45.
    doi: 10.1111/j.2042-3306.1988.tb01451.xpubmed: 3366105google scholar: lookup
  51. Nkuimi Wandjou JG, Lancioni L, Barbalace MC, Hrelia S, Papa F, Sagratini G, Vittori S, Dall’Acqua S, Caprioli G, Beghelli D. Comprehensive Characterization of Phytochemicals and Biological Activities of the Italian Ancient Apple ‘Mela Rosa Dei Monti Sibillini’. Food Res. Int. 2020;137:109422.
    doi: 10.1016/j.foodres.2020.109422pubmed: 33233104google scholar: lookup
  52. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods 2001;25:402–408.
    doi: 10.1006/meth.2001.1262pubmed: 11846609google scholar: lookup
  53. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radic. Biol. Med. 1999;26:1231–1237.
    doi: 10.1016/S0891-5849(98)00315-3pubmed: 10381194google scholar: lookup
  54. Bianchi L, Gagliardi A, Campanella G, Landi C, Capaldo A, Carleo A, Armini A, De Leo V, Piomboni P, Focarelli R. A Methodological and Functional Proteomic Approach of Human Follicular Fluid En Route for Oocyte Quality Evaluation. J. Proteom. 2013;90:61–76.
    doi: 10.1016/j.jprot.2013.02.025pubmed: 23500131google scholar: lookup
  55. Beghelli D, Zallocco L, Barbalace MC, Paglia S, Strocchi S, Cirilli I, Marzano V, Putignani L, Lupidi G, Hrelia S. Pterostilbene Promotes Mean Lifespan in Both Male and Female Drosophila Melanogaster Modulating Different Proteins in the Two Sexes. Oxidative Med. Cell. Longev. 2022;2022:1–21.
    doi: 10.1155/2022/1744408pmc: PMC8865974pubmed: 35222791google scholar: lookup
  56. Lacerenza S, Ciregia F, Giusti L, Bonotti A, Greco V, Giannaccini G, D’Antongiovanni V, Fallahi P, Pieroni L, Cristaudo A. Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome. Cancer Genom. Proteom. 2020;17:225–236.
    doi: 10.21873/cgp.20183pmc: PMC7259891pubmed: 32345664google scholar: lookup
  57. Ciregia F, Giusti L, Da Valle Y, Donadio E, Consensi A, Giacomelli C, Sernissi F, Scarpellini P, Maggi F, L쫌hini A. A Multidisciplinary Approach to Study a Couple of Monozygotic Twins Discordant for the Chronic Fatigue Syndrome: A Focus on Potential Salivary Biomarkers. J. Transl. Med. 2013;11:243.
    doi: 10.1186/1479-5876-11-243pmc: PMC3850462pubmed: 24088505google scholar: lookup
  58. Barbalace MC, Zallocco L, Beghelli D, Ronci M, Scortichini S, Digiacomo M, Macchia M, Mazzoni MR, Fiorini D, L쫌hini A. Antioxidant and Neuroprotective Activity of Extra Virgin Olive Oil Extracts Obtained from Quercetano Cultivar Trees Grown in Different Areas of the Tuscany Region (Italy) Antioxidants. Antioxidants 2021;10:421.
    doi: 10.3390/antiox10030421pmc: PMC8000409pubmed: 33801925google scholar: lookup
  59. Płóciennikowska A, Hromada-Judycka A, Borzęcka K, Kwiatkowska K. Co-Operation of TLR4 and Raft Proteins in LPS-Induced pro-Inflammatory Signaling. Cell. Mol. Life Sci. 2015;72:557–581.
    doi: 10.1007/s00018-014-1762-5pmc: PMC4293489pubmed: 25332099google scholar: lookup
  60. Liburt NR, McKeever KH, Streltsova JM, Franke WC, Gordon ME, Manso Filho HC, Horohov DW, Rosen RT, Ho CT, Singh AP. Effects of Ginger and Cranberry Extracts on the Physiological Response to Exercise and Markers of Inflammation in Horses. Comp. Exerc. Physiol. 2009;6:157–169.
    doi: 10.1017/S175525401000005Xgoogle scholar: lookup
  61. Liburt NR, Adams AA, Betancourt A, Horohov DW, McKEEVER KH. Exercise-Induced Increases in Inflammatory Cytokines in Muscle and Blood of Horses: Muscle Cytokine Response in Horses. Equine Vet. J. 2010;42:280–288.
    doi: 10.1111/j.2042-3306.2010.00275.xpubmed: 21059019google scholar: lookup
  62. Iyer SS, Cheng G. Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune Disease. Crit. Rev. Immunol. 2012;32:23–63.
    doi: 10.1615/CritRevImmunol.v32.i1.30pmc: PMC3410706pubmed: 22428854google scholar: lookup
  63. Doyle SE, Vaidya SA, O’Connell R, Dadgostar H, Dempsey PW, Wu T-T, Rao G, Sun R, Haberland ME, Modlin RL. IRF3 Mediates a TLR3/TLR4-Specific Antiviral Gene Program. Immunity 2002;17:251–263.
    doi: 10.1016/S1074-7613(02)00390-4pubmed: 12354379google scholar: lookup
  64. Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S. IL-27 Synthesis Induced by TLR Ligation Critically Depends on IFN Regulatory Factor 3. J. Immunol. 2007;178:7607–7615.
    doi: 10.4049/jimmunol.178.12.7607pubmed: 17548596google scholar: lookup
  65. Howes A, Taubert C, Blankley S, Spink N, Wu X, Graham CM, Zhao J, Saraiva M, Ricciardi-Castagnoli P, Bancroft GJ. Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. J. Immunol. 2016;197:2838–2853.
    doi: 10.4049/jimmunol.1501923pmc: PMC5026030pubmed: 27549173google scholar: lookup
  66. Coutinho-Wolino KS, Almeida PP, Mafra D, Stockler-Pinto MB. Bioactive Compounds Modulating Toll-like 4 Receptor (TLR4)-Mediated Inflammation: Pathways Involved and Future Perspectives. Nutr. Res. 2022;107:96–116.
    doi: 10.1016/j.nutres.2022.09.001pubmed: 36209684google scholar: lookup
  67. Saleh HA, Yousef MH, Abdelnaser A. The Anti-Inflammatory Properties of Phytochemicals and Their Effects on Epigenetic Mechanisms Involved in TLR4/NF-ΚB-Mediated Inflammation. Front. Immunol. 2021;12:606069.
    doi: 10.3389/fimmu.2021.606069pmc: PMC8044831pubmed: 33868227google scholar: lookup
  68. Romano C, Del Mastro A, Sellitto A, Solaro E, Esposito S, Cuomo G. Tocilizumab Reduces Complement C3 and C4 Serum Levels in Rheumatoid Arthritis Patients. Clin. Rheumatol. 2018;37:1695–1700.
    doi: 10.1007/s10067-018-3992-7pubmed: 29362962google scholar: lookup
  69. Barclay AN. Ig-like Domains: Evolution from Simple Interaction Molecules to Sophisticated Antigen Recognition. Proc. Natl. Acad. Sci. USA 1999;96:14672–14674.
    doi: 10.1073/pnas.96.26.14672pmc: PMC33953pubmed: 10611269google scholar: lookup
  70. Demaria S, Schwab R, Gottesman SR, Bushkin Y. Soluble Beta 2-Microglobulin-Free Class I Heavy Chains Are Released from the Surface of Activated and Leukemia Cells by a Metalloprotease. J. Biol. Chem. 1994;269:6689–6694.
    doi: 10.1016/S0021-9258(17)37430-6pubmed: 8120026google scholar: lookup
  71. Bouillon R, Schuit F, Antonio L, Rastinejad F. Vitamin D Binding Protein: A Historic Overview. Front. Endocrinol. 2020;10:910.
    doi: 10.3389/fendo.2019.00910pmc: PMC6965021pubmed: 31998239google scholar: lookup
  72. Haridas V, Shetty P, Sarathkumar E, Bargale A, Vishwanatha JK, Patil V, Dinesh US. Reciprocal Regulation of Pro-Inflammatory Annexin A2 and Anti-Inflammatory Annexin A1 in the Pathogenesis of Rheumatoid Arthritis. Mol. Biol. Rep. 2019;46:83–95.
    doi: 10.1007/s11033-018-4448-5pubmed: 30426384google scholar: lookup
  73. Kanters E, van Rijssel J, Hensbergen PJ, Hondius D, Mul FPJ, Deelder AM, Sonnenberg A, van Buul JD, Hordijk PL. Filamin B Mediates ICAM-1-Driven Leukocyte Transendothelial Migration. J. Biol. Chem. 2008;283:31830–31839.
    doi: 10.1074/jbc.M804888200pubmed: 18809679google scholar: lookup
  74. Nakamura F, Osborn TM, Hartemink CA, Hartwig JH, Stossel TP. Structural Basis of Filamin A Functions. J. Cell Biol. 2007;179:1011–1025.
    doi: 10.1083/jcb.200707073pmc: PMC2099194pubmed: 18056414google scholar: lookup
  75. Piktel E, Levental I, Durnaś B, Janmey P, Bucki R. Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target. Int. J. Mol. Sci. 2018;19:2516.
    doi: 10.3390/ijms19092516pmc: PMC6164782pubmed: 30149613google scholar: lookup
  76. DiNubile MJ. Plasma Gelsolin as a Biomarker of Inflammation. Arthritis Res. Ther. 2008;10:124.
    doi: 10.1186/ar2547pmc: PMC2656232pubmed: 19105851google scholar: lookup
  77. Jackson AO, Rahman GA, Long S. Apolipoprotein-AI and AIBP Synergetic Anti-Inflammation as Vascular Diseases Therapy: The New Perspective. Mol. Cell Biochem. 2021;476:3065–3078.
    doi: 10.1007/s11010-020-04037-6pubmed: 33811580google scholar: lookup
  78. Yao X, Gordon EM, Figueroa DM, Barochia AV, Levine SJ. Emerging Roles of Apolipoprotein E and Apolipoprotein A-I in the Pathogenesis and Treatment of Lung Disease. Am. J. Respir. Cell Mol. Biol. 2016;55:159–169.
    doi: 10.1165/rcmb.2016-0060TRpmc: PMC4979372pubmed: 27073971google scholar: lookup
  79. Navab M, Anantharamaiah G, Fogelman A. The Role of High-Density Lipoprotein in Inflammation. Trends Cardiovasc. Med. 2005;15:158–161.
    doi: 10.1016/j.tcm.2005.05.008pubmed: 16099381google scholar: lookup
  80. Voegele AF, Jerković L, Wellenzohn B, Eller P, Kronenberg F, Liedl KR, Dieplinger H. Characterization of the Vitamin E-Binding Properties of Human Plasma Afamin. Biochemistry 2002;41:14532–14538.
    doi: 10.1021/bi026513vpubmed: 12463752google scholar: lookup
  81. Köninger A, Edimiris P, Koch L, Enekwe A, Lamina C, Kasimir-Bauer S, Kimmig R, Dieplinger H. Serum Concentrations of Afamin Are Elevated in Patients with Polycystic Ovary Syndrome. Endocr. Connect. 2014;3:120–126.
    doi: 10.1530/EC-14-0053pmc: PMC4073231pubmed: 24928911google scholar: lookup
  82. Pennington PM, Splan RK, Jacobs RD, Chen Y, Singh RP, Li Y, Gucek M, Wagner AL, Freeman EW, Pukazhenthi BS. Influence of Metabolic Status and Diet on Early Pregnant Equine Histotroph Proteome: Preliminary Findings. J. Equine Vet. Sci. 2020;88:102938.
    doi: 10.1016/j.jevs.2020.102938pubmed: 32303306google scholar: lookup
  83. Finney J, Moon H-J, Ronnebaum T, Lantz M, Mure M. Human Copper-Dependent Amine Oxidases. Arch. Biochem. Biophys. 2014;546:19–32.
    doi: 10.1016/j.abb.2013.12.022pmc: PMC5995473pubmed: 24407025google scholar: lookup
  84. Jalkanen S, Karikoski M, Mercier N, Koskinen K, Henttinen T, Elima K, Salmivirta K, Salmi M. The Oxidase Activity of Vascular Adhesion Protein-1 (VAP-1) Induces Endothelial E- and P-Selectins and Leukocyte Binding. Blood 2007;110:1864–1870.
    doi: 10.1182/blood-2007-01-069674pubmed: 17548577google scholar: lookup
  85. Vandooren J, Itoh Y. Alpha-2-Macroglobulin in Inflammation, Immunity and Infections. Front. Immunol. 2021;12:803244.
    doi: 10.3389/fimmu.2021.803244pmc: PMC8712716pubmed: 34970276google scholar: lookup
  86. Baker SK, Strickland S. A Critical Role for Plasminogen in Inflammation. J. Exp. Med. 2020;217:e20191865.
    doi: 10.1084/jem.20191865pmc: PMC7144526pubmed: 32159743google scholar: lookup
  87. Chekol Abebe E, Tilahun Muche Z, Behaile T/Mariam A, Mengie Ayele T, Mekonnen Agidew M, Teshome Azezew M, Abebe Zewde E, Asmamaw Dejenie T, Asmamaw Mengstie M. The Structure, Biosynthesis, and Biological Roles of Fetuin-A: A Review. Front. Cell Dev. Biol. 2022;10:945287.
    doi: 10.3389/fcell.2022.945287pmc: PMC9340150pubmed: 35923855google scholar: lookup
  88. Celkan T. Plasminogen Deficiency. J. Thromb. Thrombolysis. 2017;43:132–138.
    doi: 10.1007/s11239-016-1416-6pubmed: 27629020google scholar: lookup
  89. Keragala CB, Medcalf RL. Plasminogen: An Enigmatic Zymogen. Blood 2021;137:2881–2889.
    doi: 10.1182/blood.2020008951pubmed: 33735914google scholar: lookup
  90. Öner-İyidoğan Y, Koçak H, Seyidhanoğlu M, Gürdöl F, Gülçubuk A, Yildirim F, Çevik A, Uysal M. Curcumin Prevents Liver Fat Accumulation and Serum Fetuin-A Increase in Rats Fed a High-Fat Diet. J. Physiol. Biochem. 2013;69:677–686.
    doi: 10.1007/s13105-013-0244-9pubmed: 23430567google scholar: lookup
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