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Life (Basel, Switzerland)2023; 13(3); doi: 10.3390/life13030750

Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression.

Abstract: Intense exercise can cause inflammation and oxidative stress due to the production of reactive oxygen species. These pathophysiological processes are interdependent, and each one can induce the other, creating a vicious circle. A placebo-controlled blind study was carried out in show jumping horses (n. 16) to evaluate the effects of a commercial dietary supplement (Dolhorse® N.B.F. Lanes srl, Milan, Italy) containing Verbascum thapsus leaf powder (1.42%), Curcuma longa (14.280 mg/kg), and Boswellia serrata (Roxb ex Colebr) (14.280 mg/kg) extracts. Before and after 10 days of dietary supplementation, blood samples were collected to evaluate the protein levels, antioxidants, and inflammatory responses by proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (real-time RT-PCR). A total of 36 protein spots, connected to 29 proteins, were modulated by dietary supplementation, whereas real-time RT-PCR revealed a significant downregulation of proinflammatory cytokines (interleukin 1α (p < 0.05) and interleukin-6 (0.005), toll-like receptor 4 (p < 0.05), and IKBKB (p < 0.05) in supplemented sport horses. Immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein AIV, and filamin B were overexpressed, whereas haptoglobin, α-2-HS-glycoprotein, α2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced. No effect was observed on the antioxidant defense systems. The present results suggest this phytotherapy may reinforce the innate immune responses, thus representing a valid adjuvant to alleviate inflammation, which is a pathophysiological process in sport horses.
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Publication Date: 2023-03-10 PubMed ID: 36983904PubMed Central: PMC10055707DOI: 10.3390/life13030750Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article examines the impact of certain dietary supplements on the health of show jumping horses, with a specific focus on inflammation and oxidative stress caused by intense physical exercise.

Research Aim and Methodology

  • The study aims to evaluate the effects of a commercial dietary supplement containing Verbascum thapsus leaf powder, Curcuma longa, and Boswellia serrata extracts on intense exercise-induced inflammation and oxidative stress in show jumping horses.
  • Researchers employed a placebo-controlled blind study involving 16 show jumping horses.
  • Statistics were sampled before and after ten days of supplementing the horses’ diets, with the gathered data including protein levels, antioxidant measures, and inflammatory responses. These metrics were all gauged through proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).

Results

  • Supplementing the horses’ diets resulted in the modulation of 36 protein spots related to 29 proteins.
  • Real-time RT-PCR revealed a significant decrease in proinflammatory cytokines (interleukin 1α and interleukin-6), toll-like receptor 4, and IKBKB in the supplemented horses.
  • Several proteins like immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein IV, and filamin B were overexpressed post supplementation.
  • At the same time, proteins such as haptoglobin, α-2-HS-glycoprotein, α2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced.
  • There was no noticeable impact on antioxidant defense systems.

Conclusion

  • The findings from this study suggest that the dietary supplement could potentially fortify the horses’ innate immune responses, representing a valuable addition to mitigate inflammation, a pathophysiological behavior associated with sports horses.
  • However, the study did not notably affect the horses’ antioxidant defense systems.

Overall, this research could have implications for the welfare and performance of sport horses around the world. Further research may be necessary to confirm the findings and fully understand the effects of these dietary supplements on antioxidant defense systems.

Cite This Article

APA
Beghelli D, Zallocco L, Angeloni C, Bistoni O, Ronci M, Cavallucci C, Mazzoni MR, Nuccitelli A, Catalano C, Hrelia S, L쫌hini A, Giusti L. (2023). Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression. Life (Basel), 13(3). https://doi.org/10.3390/life13030750

Publication

Life (Basel, Switzerland)
ISSN: 2075-1729
NlmUniqueID: 101580444
Country: Switzerland
Language: English
Volume: 13
Issue: 3

Researcher Affiliations

Beghelli, Daniela
  • School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Zallocco, Lorenzo
  • Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy.
Angeloni, Cristina
  • Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy.
Bistoni, Onelia
  • Rheumatology Unit, Department of Medicine, University of Perugia, 06126 Perugia, Italy.
Ronci, Maurizio
  • Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, 66100 Chieti, Italy.
Cavallucci, Clarita
  • Independent Researcher, 06083 Perugia, Italy.
Mazzoni, Maria Rosa
  • Department of Pharmacy, University of Pisa, 56126 Pisa, Italy.
Nuccitelli, Anna
  • School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Catalano, Chiara
  • Independent Researcher, 36023 Vicenza, Italy.
Hrelia, Silvana
  • Department for Life Quality Studies, Alma Mater Studiorum, University of Bologna, 47921 Rimini, Italy.
L쫌hini, Antonio
  • Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy.
Giusti, Laura
  • School of Pharmacy, University of Camerino, 62032 Camerino, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

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