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Journal of applied genetics2018; 59(2); 225-230; doi: 10.1007/s13353-018-0437-6

Differences between horse selection based on two forms of osteochondrosis in fetlock.

Abstract: Horses lose potential opportunities because of health problems. Available breeding strategies are not effective enough, probably also because of the different definition used and its genetic usefulness. The aim of the study was to compare the genetic background estimated by the genome-wide association study (GWAS) for osteochondrosis using two different scaling osteochondrosis (OC)/healthy and osteochondrosis dissecans (OCD)/healthy systems for evaluating the disease status of investigated fetlock joints. Two hundred one Warmblood horses trained for performance tests (87 stallions and 114 mares) were phenotyped and genotyped. Four fetlock x-ray images per horse were collected using the RTG Girth HF 80 and Vet Scan ray 3600. The DNA of each horse was genotyped using the BeadChip 70K. To identify SNPs that significantly affect the probability of osteochondrosis, two different methods were applied: the Cochran-Armitage test based on an additive mode of inheritance and logistic regression. The genetic background for osteochondrosis, expressed in the number of SNPs found with significant associations with osteochondrosis, was higher by evaluation in the scale of OCD/healthy horses (16 SNPs on several chromosomes mainly on the ECA1 and ECA10) than OC/healthy (2 SNPs on the ECA15 and one SNP on the ECA10). Detailed definition of osteochondrosis is needed in breeding and in veterinary practice. The genetic background for osteochondrosis and osteochondrosis dissecans seems not the same. Suggestive SNPs could be the candidate markers for osteochondrosis but should be checked on a larger population before usage.
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Publication Date: 2018-03-09 PubMed ID: 29524049PubMed Central: PMC5895668DOI: 10.1007/s13353-018-0437-6Google Scholar: Lookup
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Summary

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The research aimed to compare the genetic markers for two forms of osteochondrosis in horses using a genome-wide association study. The study found different genetic backgrounds and markers associated with osteochondrosis and osteochondrosis dissecans, suggesting the need for a more detailed definition for each in breeding and veterinary practices.

Research Objective and Methodology

The objective of the research was to:

  • Compare the estimated genetic backgrounds of osteochondrosis (OC) and osteochondrosis dissecans (OCD) in the fetlock joints of Warmblood horses.
  • Improve existing breeding strategies by better understanding the genetic underpinnings of these frequently encountered health issues in horses.

The methodology involved:

  • Phenotyping and genotyping 201 Warmblood horses (87 stallions and 114 mares) conditioned for performance tests.
  • Collecting four fetlock x-ray images per horse using RTG Girth HF 80 and Vet Scan ray 3600.
  • Genotyping the DNA of each horse using the BeadChip 70K.
  • Analyzing the data using two methods: the Cochran-Armitage test (based on an additive mode of inheritance) and logistic regression to identify SNPs (single-nucleotide polymorphisms), genetic variants that significantly affect osteochondrosis.

Results

The analysis showed:

  • 16 SNPs associated with OCD on several chromosomes, mainly on ECA1 and ECA10.
  • Only two SNPs associated with OC on ECA15 and one SNP on ECA10.

Implications

These findings indicate that:

  • The genetic backgrounds for OC and OCD are not identical, suggesting different markers needed for each in breeding and veterinary practices.
  • There could be a need for a more precise definition of OC and OCD in the breeding industry and veterinary practices to better adapt and implement breeding strategies and healthcare respectively.
  • The identified SNPs could be candidate markers for osteochondrosis, but need validation on a larger population before being used in practice.

Cite This Article

APA
Lewczuk D, Bereznowski A, Hecold M, Frąszczak M, Ruść A, Korwin-Kossakowska A, Szyda J, Kamiński S. (2018). Differences between horse selection based on two forms of osteochondrosis in fetlock. J Appl Genet, 59(2), 225-230. https://doi.org/10.1007/s13353-018-0437-6

Publication

Journal of applied genetics
ISSN: 2190-3883
NlmUniqueID: 9514582
Country: England
Language: English
Volume: 59
Issue: 2
Pages: 225-230

Researcher Affiliations

Lewczuk, D
  • Institute of Genetics and Animal Breeding PAS, ul.Postępu 36A, 05-552, Magdalenka, Poland. d.lewczuk@ighz.pl.
Bereznowski, A
  • Warsaw University of Life Science-SGGW, ul.Nowoursynowska 166, 02-787, Warsaw, Poland.
Hecold, M
  • Warsaw University of Life Science-SGGW, ul.Nowoursynowska 166, 02-787, Warsaw, Poland.
Frąszczak, M
  • University of Environmental and Life Science, ul.CK Norwida 25/27, 50-375, Wrocław, Poland.
Ruść, A
  • University of Warmia and Mazury, ul.Oczapowskiego 5, 10-719, Olsztyn, Poland.
Korwin-Kossakowska, A
  • Institute of Genetics and Animal Breeding PAS, ul.Postępu 36A, 05-552, Magdalenka, Poland.
Szyda, J
  • University of Environmental and Life Science, ul.CK Norwida 25/27, 50-375, Wrocław, Poland.
Kamiński, S
  • University of Warmia and Mazury, ul.Oczapowskiego 5, 10-719, Olsztyn, Poland.

MeSH Terms

  • Animals
  • Female
  • Genome-Wide Association Study
  • Genotype
  • Horse Diseases / genetics
  • Horses / genetics
  • Male
  • Osteochondrosis / genetics
  • Osteochondrosis / veterinary
  • Phenotype
  • Polymorphism, Single Nucleotide

Grant Funding

  • 2011/01/B/NZ2/00893 / National Science Centre (NCN)
  • NR 12 0037 06. / National Centre for Research and Development (NCBiR)

Conflict of Interest Statement

CONFLICT OF INTEREST: The authors declare that they have no conflict of interest. ETHICAL STATEMENT: All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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